Determination By X-Ray Crystallography of the Three Dimensional- Structure of Acetylcholinesterase from Torpedo Electric Organ

1993 ◽  
Author(s):  
J. L. Sussman ◽  
I. Silman



Functional studies on interferon would be helped by a three-dimensional structure for the molecule. However, it may be several years before the structure of the protein is determined by X-ray crystallography. We have therefore used available methods for predicting the secondary - and the tertiary - structure of a protein from its amino acid sequence to propose a tertiary model involving the packing of four a-helices. Details of this work have been published elsewhere (Sternberg & Cohen 1982).





Author(s):  
Qingqiu Huang ◽  
Doletha M. E. Szebenyi

The production of high-quality crystals is one of the major obstacles in determining the three-dimensional structure of macromolecules by X-ray crystallography. It is fairly common that a visually well formed crystal diffracts poorly to a resolution that is too low to be suitable for structure determination. Dehydration has proven to be an effective post-crystallization treatment for improving crystal diffraction quality. Several dehydration methods have been developed, but no single one of them is suitable for all crystals. Here, a new convenient and effective dehydration method is reported that makes use of a dehydrating solution that will not dry out in air for several hours. Using this dehydration method, the resolution ofArchaeoglobus fulgidusCas5a crystals has been increased from 3.2 to 1.95 Å and the resolution ofEscherichia coliLptA crystals has been increased from <5 to 3.4 Å.



Biochemistry ◽  
1991 ◽  
Vol 30 (15) ◽  
pp. 3739-3748 ◽  
Author(s):  
Roland K. Strong ◽  
Robert Campbell ◽  
David R. Rose ◽  
Gregory A. Petsko ◽  
Jacqueline Sharon ◽  
...  


1988 ◽  
Vol 16 (6) ◽  
pp. 949-953 ◽  
Author(s):  
JOHN P. PRIESTLE ◽  
HANS-PETER SCHÄR ◽  
MARKUS G. GRÜTTER

Summary The three-dimensional structure of human recombinant interleukin-1β has been determined at 0.24 nm resolution by X-ray crystallographic techniques. The partially refined model has a crystallographic R-factor of just under 19%. The structure is composed of 12 β-strands forming a complex network of hydrogen bonds. The core of the structure can best be described as a tetrahedron whose edges are each formed by two antiparallel β-strands. The interior of this structure is filled with hydrophobic side-chains. There is a 3-fold repeat in the folding of the polypeptide chain. Although this folding pattern suggests gene triplication, no significant internal sequence homology between topologically corresponding residues exists. The folding topology of interleukin-1β is very similar to that described by A. D. McLachlan [(1979) J. Mol. Biol. 133, 557–563] for soybean trypsin inhibitor.



Materia Japan ◽  
2007 ◽  
Vol 46 (12) ◽  
pp. 823-823
Author(s):  
Seiji Kawado ◽  
Toshinori Taishi ◽  
Satoshi Iida ◽  
Yoshifumi Suzuki ◽  
Yoshinori Chikaura ◽  
...  


2004 ◽  
Vol 693 (1-3) ◽  
pp. 199-203 ◽  
Author(s):  
Quan-Zheng Zhang ◽  
Can-Zhong Lu ◽  
Wen-Bin Yang ◽  
Shu-Mei Chen ◽  
Ya-Qin Yu


2004 ◽  
Vol 573 (2) ◽  
pp. 191-203 ◽  
Author(s):  
P. Geissbühler ◽  
P. Fenter ◽  
E. DiMasi ◽  
G. Srajer ◽  
L.B. Sorensen ◽  
...  


2021 ◽  
Author(s):  
Xianke Wang ◽  
Lin Chen ◽  
Bin Huang ◽  
Jin Yuan ◽  
Lili Shang ◽  
...  

Abstract Bamboo is a natural fiber composite with layered structure. Millions of years of evolution have endowed bamboo with the most effective structure in nature. The ingenious microstructure provides bamboo with excellent mechanical properties. Bamboo culm is composed of the cortex, a middle layer, and a pith ring. The cortex refers to the area starting from the periphery of the culm wall to the vascular bundles. The present study obtained the two-dimensional microstructure of bamboo cortex cells by optical microscopy and characterized the three-dimensional structure through high-resolution X-ray microtomography (µCT). Based on the analysis, the bamboo cortex cells were classified into four layers: epidermis layer, hypodermis layer, transitional layer, and parenchyma layer. The average pore volume of the bamboo cortex was about 1.54×10-6 mm3, the porosity was 36.1%, and the relative density was 0.639. The epidermis layer, hypodermis layer, transition layer, and parenchyma layer cells had a cell cavity volume of 917.81 µm3, 714.22 µm3, 1258.19 µm3, and 3117.65 µm3, respectively, an average length3d (L) of 19.38 µm, 25.84 µm, 26.46 µm, and 34.88 µm, respectively, an average breadth3d (W) of 14.11 µm, 9.44 µm, 15.22 µm, and 16.6 µm, respectively, and sphericity of 0.85, 0.76, 0.75, and 0.78, respectively. Studies on bamboo anatomical structure, especially three-dimensional digital characterization, will enrich the bamboo microstructure database. Besides, the three-dimensional structure of the bamboo cortex revealed in this study can provide a reference for optimizing composite material hierarchy and biomimetic design.



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