Tumor-Secreted Autocrine Motility Factor (AMF): Causal Role in an Animal

2006 ◽  
Author(s):  
John M. Chirgwin
Keyword(s):  
Causal Role ◽  
Autocrine Motility Factor ◽  
Motility Factor

Expression of autocrine motility factor receptor in colorectal cancer as a predictor for disease recurrence

Cancer ◽  
1994 ◽  
Vol 74 (7) ◽  
pp. 1855-1862 ◽  
Author(s):  
Shoji Nakamori ◽  
Hideomi Watanabe ◽  
Masao Kameyama ◽  
Shingi Imaoka ◽  
Hiroshi Furukawa ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Disease Recurrence ◽  
Autocrine Motility Factor ◽  
Motility Factor

Clathrin-mediated endocytosis and recycling of autocrine motility factor receptor to fibronectin fibrils is a limiting factor for NIH-3T3 cell motility

2000 ◽  
Vol 113 (18) ◽  
pp. 3227-3240 ◽  
Author(s):  
P.U. Le ◽  
N. Benlimame ◽  
A. Lagana ◽  
A. Raz ◽  
I.R. Nabi
Keyword(s):  
Cell Surface ◽  
Cell Motility ◽  
Endocytic Pathway ◽  
Limiting Factor ◽  
Autocrine Motility Factor ◽  
Endocytic Compartment ◽  
Motility Factor ◽  
Microtubule Disruption ◽  
Nih 3T3 ◽  
Fibronectin Fibrils

Autocrine motility factor receptor (AMF-R) is internalized via a clathrin-independent pathway to smooth endoplasmic reticulum tubules. This endocytic pathway is shown here to be inhibited by methyl-(beta)-cyclodextrin (m(beta)CD) implicating caveolae or caveolae-like structures in AMF internalization to smooth ER. AMF-R is also internalized via a clathrin-dependent pathway to a transferrin receptor-negative, LAMP-1/lgpA-negative endocytic compartment identified by electron microscopy as a multivesicular body (MVB). Endocytosed AMF recycles to cell surface fibrillar structures which colocalize with fibronectin; AMF-R recycling is inhibited at 20 degrees C, which blocks endocytosis past the early endosome, but not by m(beta)CD demonstrating that AMF-R recycling to fibronectin fibrils is mediated by clathrin-dependent endocytosis to MVBs. Microtubule disruption with nocodazole did not affect delivery of bAMF to cell surface fibrils indicating that recycling bAMF traverses the MVB but not a later endocytic compartment. Plating NIH-3T3 cells on an AMF coated substrate did not specifically affect cell adhesion but prevented bAMF delivery to cell surface fibronectin fibrils and reduced cell motility. AMF-R internalization and recycling via the clathrin-mediated pathway are therefore rate-limiting for cell motility. This recycling pathway to the site of deposition of fibronectin may be implicated in the de novo formation of cellular attachments or the remodeling of the extracellular matrix during cell movement.

scholarly journals Higher autocrine motility factor/glucose-6-phosphate isomerase expression is associated with tumorigenesis and poorer prognosis in gastric cancer

2018 ◽  
Vol Volume 10 ◽  
pp. 4969-4980 ◽  
Author(s):  
Yuteng Ma ◽  
Xiaofang Xing ◽  
Bin Dong ◽  
Xiaojing Cheng ◽  
Ting Guo ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Autocrine Motility Factor ◽  
Motility Factor

Detection of Autocrine Motility Factor in Urine as a Marker of Bladder Cancer

1988 ◽  
Vol 80 (15) ◽  
pp. 1203-1211 ◽  
Author(s):  
R. Guirguis ◽  
E. Schiffmann ◽  
B. Liu ◽  
D. Birkbeck ◽  
J. Engel ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Autocrine Motility Factor ◽  
Motility Factor
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