scholarly journals In Vitro Micropropagation of 54 Species from 15 Genera of Bamboo

HortScience ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 453-454 ◽  
Author(s):  
P. Prutpongse ◽  
P. Gavinlertvatana
Keyword(s):  
Room Temperature ◽  
Multiple Shoots ◽  
Axillary Buds ◽  
Skoog Medium ◽  
Stem Node ◽  
In Vitro Micropropagation ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Strength Medium

Fifty-four out of 67 species of bamboo tested were successfully propagated in vitro. For nearly every species, multiple shoots were produced from axillary buds on stem node segments cultured on Murashige and Skoog medium containing BA. In a very few species plants could be regenerated adventitiously from callus. This method of propagation was not very efficient or reliable. Rooting occurred in media containing NAA at 2.7 to 5.4 μM. Several species could be stored in vitro on half-strength medium at room temperature > 15 months without transfer. Chemical names used: N6-benzylamino purine (BA); napthyleneacetic acid (NAA).

scholarly journals Some preliminary results of experiments with in-vitro culture of dipterocarps.

1983 ◽  
Vol 31 (3) ◽  
pp. 233-238
Author(s):  
W.T.M. Smits ◽  
B. Struycken
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Leaf Explants ◽  
Axillary Buds ◽  
Parent Plant ◽  
Preliminary Results ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Strength Medium

A study was made of the influence of light, temp., growth regulators, and sugar and macrosalt concn. on the growth and morphogenesis of leaf and axis explants of young Shorea curtisii, S. obtusa and Dipterocarpus grandiflorus. Terminal and axillary buds grew best on half strength Murashige and Skoog medium. Leaf explants formed more callus on full strength medium, when containing part of the midrib and when taken from the lower half of the leaf. More than 95% of D. grandiflorus explants were infected by a fungus apparently present in the parent plant. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Effects of Cytokinin on in vitro Propagation of Bauhinia variegata L.

2020 ◽  
Vol 1 (6) ◽  
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
In Vitro Propagation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Nodal Cuttings ◽  
Half Strength ◽  
Bauhinia Variegata ◽  
Mature Seeds

Mature seeds of Bauhinia variegata L were cultured on half strength Murashige and Skoog medium. For experimentation, nodal cuttings were used as explants from in vitro growing plants. Cytokinin, N-benzyl-9-(2-tetrahydropyranyl) (BPA), kinetin(6-furfurylaminopurine), zeatin, 6-(4-hydroxy-3-methyl-trans -2-butenyl amino purine), 2- isopentenyl amino purine (2-ip), and benzylaminopurine (BAP) were tested for best propagation. Well grown plants were achieved in medium supplemented with 5 µM BPA and 0.5 µM BAP. The propagated plants were acclimatized very well after transferred to the field.

scholarly journals In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation

2018 ◽  
Vol 54 (No. 4) ◽  
pp. 183-189
Author(s):  
Pranit Kumar Mukherjee ◽  
Raju Mondal ◽  
Sourav Dutta ◽  
Kanti Meena ◽  
Madhumita Roy ◽  
...  
Keyword(s):  
Large Scale ◽  
Random Amplified Polymorphic Dna ◽  
Ectopic Expression ◽  
Skoog Medium ◽  
Boehmeria Nivea ◽  
In Vitro Micropropagation ◽  
Murashige And Skoog Medium ◽  
Planting Materials ◽  
Large Scale Cultivation

An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO<sub>3</sub>. ½ Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expression in respect of any morphological character in comparison with the parental stock. Furthermore, clonal fidelity of the mericlones was confirmed by using DNA markers (random amplified polymorphic DNA and inter simple sequence repeats) and by polypeptide profiling through SDS-PAGE at a genomic and protein level, respectively, which showed the true-to-type nature of the in vitro micropropagated plants. Thus the protocol developed can be used to generate safe planting material for large-scale cultivation of ramie.  

scholarly journals Micropropagation of Cascade Huckleberry, Mountain Huckleberry, and Oval-leaf Bilberry Using Woody Plant Medium and Murashige and Skoog Medium Formulations

2007 ◽  
Vol 17 (3) ◽  
pp. 279-284 ◽  
Author(s):  
Danny L. Barney ◽  
Omar A. Lopez ◽  
Elizabeth King
Keyword(s):  
Plant Growth ◽  
Growth Regulators ◽  
Shoot Growth ◽  
Woody Plant ◽  
Skoog Medium ◽  
Average Survival ◽  
Murashige And Skoog Medium ◽  
Woody Plant Medium ◽  
Half Strength

Two concentrations of two in vitro media formulations were evaluated for their effects on survival, shoot growth, and percentage rooting of cascade huckleberry (Vaccinium deliciosum), mountain huckleberry (V. membranaceum), and oval-leaf bilberry (V. ovalifolium). Two-node stem sections from established microshoots were cultured on full- or half-strength modified Murashige and Skoog medium (FSMS and HSMS) or full- or half-strength modified woody plant medium (FSWPM and HSWPM) unamended with plant growth regulators. Cultures were maintained at 21 °C with a 16-hour photoperiod for 98 days. Survival on FSMS was reduced by ≈44% for cascade huckleberry, 63% for mountain huckleberry, and 18% for oval-leaf bilberry compared with average survival on HSMS, HSWPM, and FSWPM. Explants on FSMS also produced new shoot growth having the lowest dry weights, fewest shoots, and shortest shoots of the four media. Explant rooting percentages were also least on FSMS. For cascade huckleberry and oval-leaf bilberry, HSMS, HSWPM, and FSWPM all appeared suitable for general culture. For mountain huckleberry, both woody plant medium formulations produced greater microshoot dry weights, average shoot lengths, and explant rooting percentages compared with HSMS. These results are the first published on micropropagation for cascade huckleberry and oval-leaf bilberry, and provide starting protocols for commercial propagation and further research on micropropagation of these species.

scholarly journals In Vitro Mass Propagation of Heliotropium indicum L., using Apical and Axillary Bud Explants

1970 ◽  
Vol 45 (1) ◽  
pp. 69-74 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Nadira Begum ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Axillary Buds ◽  
Mass Propagation ◽  
Half Strength ◽  
Heliotropium Indicum ◽  
Regenerated Plantlets

A consequency was obtained for mass propagation of a valuable ayurvedic medicinal herb, Heliotropium indicum Linn. (Boraginaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 92% of the axillary buds explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 18 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Heliotropium indicum; Medicinal plant; Shoot proliferation; Micropropagation; Acclimatization. DOI: 10.3329/bjsir.v45i1.5185 Bangladesh J. Sci. Ind. Res. 45(1), 69-74, 2010

scholarly journals Clonal propagation of Carob (Ceratonia siliqua L., Fabaceae)

1970 ◽  
Vol 39 (1) ◽  
pp. 15-19 ◽  
Author(s):  
L Hakim ◽  
MR Islam ◽  
ANK Mamun ◽  
G Ahmed ◽  
R Khan
Keyword(s):  
Clonal Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Root Induction ◽  
Ms Medium ◽  
Ceratonia Siliqua ◽  
Moderate Amount ◽  
Half Strength

Mature seeds of carob tree (Ceratonia siliqua L.) were germinated on hormone free MS medium. Efforts were made to develop multiple shoots by using axillary buds of in vitro grown seedlings on MS medium fortified with different concentrations of BA singly and BA in combination with IAA or GA3. Axillary buds produced single shoot with a moderate amount of callus at the base of the explant after culturing on MS medium with BA alone. Multiple shoots were regenerated when explants when cultured on MS medium fortified with BA + IAA or BA + GA3. MS medium supplemented with 1.5 mg/l BA + 0.5 mg/l GA3 was found more effective in multiple shoot regeneration than all other combinations. Regenerated multiple shoots were excised and cultured on half strength of MS medium containing different concentrations of IBA for root induction. Best root development was obtained in half strength MS medium containing 0.5 mg/l IBA. About 70% of the regenerated plantlets survived in natural conditions. Key words: Carob; Ceratonia siliqua; Fabaceae; Clonal propagation DOI: 10.3329/bjb.v39i1.5520Bangladesh J. Bot. 39(1): 15-19, 2010 (June)

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals Research on in vitro micropropagation of Lilium brownii F.E. Brown

2016 ◽  
Vol 14 (1) ◽  
pp. 121-129
Author(s):  
Vũ Hoài Sâm ◽  
Bùi Đức Quỳnh ◽  
Nguyễn Thị Hương ◽  
Nguyễn Văn Khiêm
Keyword(s):  
Subtropical Climate ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Medicinal Species ◽  
Surface Sterilization ◽  
The North ◽  
In Vitro Micropropagation ◽  
Half Strength ◽  
Over Exploitation

Lilium brownii Brown belonging Lilium genus and Liliaceae family is well-known as a popular medicinal species, as well as food source and beautiful ornamental flowers. The specie has unique and ornamental floral characteristics such as light and elegant fragrance and perianth color rapidly changing from yellowish cream to white during anthesis. In traditional medicine, it is used for treatment cough, sedation diuretic, bronchitis... In nature, it can be found in subtropical climate moutainous areas in the North such as Sa Pa, Bat Xat, Mu Cang Chai; Sin Ho and Phong Tho, Quang Ba and Dong Van. In recent years, this species has been listed in the Red List for medicinal plants in Vietnam due to over-exploitation. The only effective strategy for sustaible conservation this species is in vitro micropropagation. In this study, in vitro plant regeneration and micropropagation of L. brownii was established from bubles and stem nodes. After surface sterilization with 0.1% HgCl­2 in 10 minutes, healthy young shoots were obtained from initial bubles and stem nodes on MS medium supplemented with 0.5 mg/l BAP or 0.5 mg/l NAA, respectively.  Bulblets also were formed from young shoot on MS supplemented with 0.5 mg/l NAA. The highest number of 4.5 bulblets per an explant was recorded from longitude-divided bubbles on MS medium containing 0.5 mg/l NAA and 0.2 mg/l BAP after 60 days in culture. The regererated plants produced quality roots on half strength MS supplemented with the combination of 1.0 mg/ l NAA and 0.2 mg / l BAP. More than 90% of rooted plants in vitro were survival on artificial soil TN1 in the nursery.

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