scholarly journals Varietal Identification of Cyclamen and Petunia Seeds Using RAPDs

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 574d-574
Author(s):  
Jianhua Zhang ◽  
Miller B. McDonald
Keyword(s):  
Molecular Biology ◽  
Genetic Variability ◽  
Seed Production ◽  
Seed Quality ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Purity ◽  
Molecular Biology Technique ◽  
Banding Patterns ◽  
Varietal Identification

Varietal identification of cyclamen and petunia is important for flower seed production because these crops are marketed as hybrids and genetic purity determinations assure the purity of the seed lot and the success of hybridization. Random amplified polymorphic DNA (RAPD) banding patterns have been shown to be useful in identifying genotypes of various crops. This molecular biology technique was applied to five commercial cyclamen and six petunia hybrids. Using bulk seed/seedling samples, the varieties could be differentiated. However, when individual seeds of the cyclamen hybrid were tested, differing polymorphisms were observed. These variations were attributed to genetic variability in the inbred parents. We conclude that the genetic purity of cyclamen seeds can be improved and that the use of RAPDs can assist breeders of hybrid flowering crops in better monitoring seed quality.

scholarly journals Seed Science and Technology. Volume 48 Issue 3 (2020)

2020 ◽  
Vol 48 (3) ◽  
pp. 439-451
Author(s):  
Julio Marcos-Filho
Keyword(s):  
Seed Production ◽  
Seed Quality ◽  
Science And Technology ◽  
Genetic Conservation ◽  
Environmental Restoration ◽  
Genetic Purity ◽  
Seed Testing ◽  
Ornamental Crops ◽  
Local Value

The quality of a seed lot results from the interaction of attributes that determine its overall value for a specific purpose. Seed Science and Technology is a widely recognised journal first published in 1973 as a continuation of the Proceedings of the International Seed Testing Association (1921 to 1972). The Journal publishes original papers and articles comprising different aspects of seed production, processing, storage, testing, genetic conservation, habitat regeneration and reforestation programs, with both basic and applied topics on seed science. Seed quality represents a permanent focus of Seed Science and Technology and the articles published in this issue, with a predominant approach on physiological potential, genetic purity and seed enhancement, confirm this strong commitment The species covered by the articles in this issue represent important worldwide grain, forage and ornamental crops, but there are also species with local value, for environmental restoration and medicinal application.

scholarly journals Testing for Genetic Purity in Petunia and Cyclamen Seed Using Random Amplified Polymorphic DNA Markers

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 246-247 ◽  
Author(s):  
Zhang Jianhua ◽  
Miller B. McDonald ◽  
Patricia M. Sweeney
Keyword(s):  
Dna Markers ◽  
Petunia Hybrida ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Cyclamen Persicum ◽  
Genetic Purity ◽  
Banding Patterns ◽  
Rapd Makers ◽  
Seed Crops

This study examined the use of random amplified polymorphic DNA (RAPD) markers as a means to identify cultivars of petunia (Petunia hybrida Vilm) seedlings and cyclamen (Cyclamen persicum Mill.) seeds and to determine the genetic purity within cyclamen seeds. Bulked samples of six petunia and five cyclamen hybrid cultivars, respectively, produced consistent RAPD marker profiles. Evaluation of individual seeds from a single cyclamen hybrid produced polymorphic banding patterns that were attributed to genetic variability present in the female and male inbred parents. These results show that RAPD makers can be used to quickly assess the genetic purity of selected cultivars of these two flower seed crops.

scholarly journals Sequence-characterized amplified region (SCAR) technique used for variety discrimination in vinca (Catharanthus roseus (L.) G.Don)

2002 ◽  
Vol 24 (1) ◽  
pp. 299-305 ◽  
Author(s):  
Carlos C.E Menezes ◽  
Tara Vantoai ◽  
Miller B McDonald ◽  
Tocio Sediyama
Keyword(s):  
Catharanthus Roseus ◽  
Seed Quality ◽  
Sequence Information ◽  
Genetic Purity ◽  
Banding Patterns ◽  
Sequence Characterized Amplified Region ◽  
Purity Testing ◽  
Polymorphic Bands ◽  
Scar Primers

Sequence-Characterized Amplified Region (SCAR) appears as a useful technique for genetic purity testing and variety discrimination, applicable to species in which some other techniques have failed. In particular, this technique is very attractive with species in which RAPD results were not consistent. The RAPD polymorphic bands were cloned, sequenced and from the sequence information, primers pairs for normal PCR were developed. Since the probability of obtaining successful SCAR primers from RAPD polymorphic bands was about 50%, a larger number of RAPD polymorphic bands are needed to develop sufficient SCAR primers for varietal discrimination in vinca. In addition, the efficiency of the SCAR technique is strongly affected by the quality of DNA extracted from seeds. The SCAR banding patterns obtained from vinca seed were consistent and repeatable making the results reliable for genetic purity testing and variety discrimination. The SCAR technique is simple, fast, relatively inexpensive and allows the use of DNA extracted from dry seeds, which is very important in a seed-quality evaluating program

scholarly journals Isolates of Uromyces appendiculatus with Specific Virulence to Landraces of Phaseolus vulgaris of Andean Origin

Plant Disease ◽  
1999 ◽  
Vol 83 (2) ◽  
pp. 108-113 ◽  
Author(s):  
Craig M. Sandlin ◽  
James R. Steadman ◽  
Carlos M. Araya ◽  
Dermot P. Coyne
Keyword(s):  
Phaseolus Vulgaris ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rust Fungus ◽  
Distinct Group ◽  
Uromyces Appendiculatus ◽  
Bean Rust ◽  
Banding Patterns ◽  
Highly Virulent ◽  
Specific Virulence

Five isolates of the bean rust fungus Uromyces appendiculatus were shown to be specifically virulent on bean genotypes of Andean origin. This specificity was demonstrated by the virulence of five pairs of isolates on a differential set of 30 Phaseolus vulgaris landraces. Each isolate pair was from a different country in the Americas and consisted of one Andean-specific isolate and one nonspecific isolate. Of the differential P. vulgaris landraces, 15 were of Middle American origin and 15 were of Andean origin. The Andean-specific rust isolates were highly virulent on Andean landraces but not on landraces of Middle American origin. Rust isolates with virulence to Middle American landraces were also generally virulent on Andean material; no truly Middle American-specific isolates were found. Random amplified polymorphic DNA (RAPD) analysis of the rust isolates also distinguished the two groups. Four of the Andean-specific rust isolates formed a distinct group compared to four of the nonspecific isolates. Two of the isolates, one from each of the two virulence groups, had intermediate RAPD banding patterns, suggesting that plasmagomy but not karyogamy occurred between isolates of the two groups.

Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 201-210 ◽  
Author(s):  
F. N. Wachira ◽  
R. Waugh ◽  
W. Powell ◽  
C. A. Hackett
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Southeast Asia ◽  
Camellia Sinensis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Systematic Assessment ◽  
Tree Crop ◽  
Taxonomic Relationships

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

scholarly journals Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India

2016 ◽  
Vol 97 (6) ◽  
pp. 1307-1315 ◽  
Author(s):  
Elangovan Dilipan ◽  
Jutta Papenbrock ◽  
Thirunavakkarasu Thangaradjou
Keyword(s):  
Genetic Variability ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Seagrass Species ◽  
Complex Species ◽  
Marine Angiosperms ◽  
Different Populations ◽  
High Degree ◽  
Identification Tool

In India 14 seagrass species can be found with monospecific genera (Enhalus, ThalassiaandSyringodium),Cymodoceawith two species andHalophilaandHalodulerepresented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of theHalophilaandHalodulecomplex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.

scholarly journals The use of random amplified polymorphic DNA to evaluate the genetic variability of Ponkan mandarin (Citrus reticulata Blanco) accessions

2000 ◽  
Vol 23 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Helvécio Della Coletta Filho ◽  
Marcos Antonio Machado ◽  
M. Luiza P.N. Targon ◽  
Jorgino Pompeu Jr.
Keyword(s):  
Genetic Variability ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Point Mutations ◽  
The Other ◽  
Citrus Reticulata ◽  
Random Primers ◽  
Citrus Reticulata Blanco ◽  
Ponkan Mandarin

RAPD analysis of 19 Ponkan mandarin accessions was performed using 25 random primers. Of 112 amplification products selected, only 32 were polymorphic across five accessions. The absence of genetic variability among the other 14 accessions suggested that they were either clonal propagations with different local names, or that they had undetectable genetic variability, such as point mutations which cannot be detected by RAPD.

Effect of method and time of application of nitrogen fertiliser on yield and quality of seed of creeping red fescue

2000 ◽  
Vol 80 (4) ◽  
pp. 809-811 ◽  
Author(s):  
N. A. Fairey ◽  
L. P. Lefkovitch
Keyword(s):  
Seed Production ◽  
Seed Yield ◽  
Seed Quality ◽  
Early Spring ◽  
Festuca Rubra ◽  
Grass Seed ◽  
Red Fescue ◽  
Time Of Application ◽  
Vigorous Plant ◽  
Tiller Density

Six trials were conducted on commercial seed fields of creeping red fescue (Festuca rubra L. var. rubra) in the Peace region to evaluate the sensitivity of seed production to the method and time of application of N fertiliser. In each trial, 68 kg ha−1 N was applied using three methods (surface-broadcast, granular, ammonium nitrate 34-0-0; foliar/soil spray of 28-0-0 solution N; soil-injected 28-0-0 solution N) at each of three times (fall, early spring, late spring). No statistically significant (P = 0.05) interaction, or main effect, of method and time of N were revealed for seed yield, fertile tiller density or for several seed quality characteristics. Seed yield varied greatly among trials (142 to 1240 kg ha−1) and averaged 566 kg ha−1. The results indicate that there is considerable flexibility in the method and time of application of N fertiliser for seed production of creeping red fescue in the Peace region, provided it is applied before the commencement of vigorous plant growth in the spring. Key words: Creeping red fescue, Festuca rubra L., nitrogen fertility, grass seed production, grass seed quality

scholarly journals Use of molecular marker techniques in seed testing by Brazilian seed companies

1998 ◽  
Vol 55 (spe) ◽  
pp. 79-82 ◽  
Author(s):  
P.T. Della Vecchia ◽  
C.A.R. da Silva ◽  
P. Terenciano-Sobrinho
Keyword(s):  
Molecular Marker ◽  
New Technologies ◽  
Seed Quality ◽  
Genetic Purity ◽  
Process Step ◽  
Seed Testing ◽  
Routine Basis ◽  
Seed Market ◽  
Key Issues ◽  
High Quality Seed

Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD, microsatelites, AFLP) for genetic purity determinations in seed testing. As far as we know, no Brazilian seed company is using, on regular basis, RAPD or other molecular marker techniques in the determination of genetic purity in seed testing. Most of these are using morphological or physiological traits expressed by seed, seedling or mature plant and/or electrophoresis of seed or seedling proteins/isoenzymes for that purpose. Main reasons for that are: DNA molecular marker techniques are relatively new; lack of specialized personnel to run DNA molecular marker assays on routine basis; higher cost/sample when compared to proteins/isoenzymes electrophoresis.

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