Genetic variability of Citrus spp. in Cibinong germplasm garden using random amplified polymorphic DNA

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

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Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315416000631 ◽

2016 ◽

Vol 97 (6) ◽

pp. 1307-1315 ◽

Cited By ~ 3

Author(s):

Elangovan Dilipan ◽

Jutta Papenbrock ◽

Thirunavakkarasu Thangaradjou

Keyword(s):

Genetic Variability ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Rapd Marker ◽

Seagrass Species ◽

Complex Species ◽

Marine Angiosperms ◽

Different Populations ◽

High Degree ◽

Identification Tool

In India 14 seagrass species can be found with monospecific genera (Enhalus, ThalassiaandSyringodium),Cymodoceawith two species andHalophilaandHalodulerepresented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of theHalophilaandHalodulecomplex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.

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The use of random amplified polymorphic DNA to evaluate the genetic variability of Ponkan mandarin (Citrus reticulata Blanco) accessions

Genetics and Molecular Biology ◽

10.1590/s1415-47572000000100031 ◽

2000 ◽

Vol 23 (1) ◽

pp. 169-172 ◽

Cited By ~ 8

Author(s):

Helvécio Della Coletta Filho ◽

Marcos Antonio Machado ◽

M. Luiza P.N. Targon ◽

Jorgino Pompeu Jr.

Keyword(s):

Genetic Variability ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Point Mutations ◽

The Other ◽

Citrus Reticulata ◽

Random Primers ◽

Citrus Reticulata Blanco ◽

Ponkan Mandarin

RAPD analysis of 19 Ponkan mandarin accessions was performed using 25 random primers. Of 112 amplification products selected, only 32 were polymorphic across five accessions. The absence of genetic variability among the other 14 accessions suggested that they were either clonal propagations with different local names, or that they had undetectable genetic variability, such as point mutations which cannot be detected by RAPD.

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Genetic variability in the natural populations of Lasioderma serricorne (F.) (Coleoptera: Anobiidae), detected by RAPD markers and by esterase isozymes

Bulletin of Entomological Research ◽

10.1017/s0007485315000723 ◽

2015 ◽

Vol 106 (1) ◽

pp. 47-53 ◽

Cited By ~ 4

Author(s):

T. Coelho-Bortolo ◽

C.A. Mangolin ◽

A.S. Lapenta

Keyword(s):

Genetic Variability ◽

Random Amplified Polymorphic Dna ◽

Natural Populations ◽

Electrophoretic Analysis ◽

Effective Control ◽

Lasioderma Serricorne ◽

Control Programs ◽

Dried Fruit ◽

High Level ◽

High Degree

AbstractLasioderma serricorne (F.) is a small cosmopolitan beetle regarded as a destructive pest of several stored products such as grains, flour, spices, dried fruit and tobacco. Chemical insecticides are one of the measures used against the pest. However, intensive insecticide use has resulted in the appearance of resistant insect populations. Therefore, for the elaboration of more effective control programs, it is necessary to know the biological aspects of L. serricorne. Among these aspects, the genetic variability knowledge is very important and may help in the development of new control methods. The objective of this study was to evaluate the genetic variability of 11 natural populations of L. serricorne collected respectively in three and four towns in the states of Paraná and São Paulo, Brazil, using 20 primers random amplified polymorphic DNA (RAPD) and polymorphisms of esterases. These primers produced 352 polymorphic bands. Electrophoretic analysis of esterases allowed the identification of four polymorphic loci (Est-2, Est-4, Est-5 and Est-6) and 18 alleles. Results show that populations are genetically differentiated and there is a high level of genetic variability within populations. The high degree of genetic differentiation is not directly correlated to geographical distance. Thus, our data indicate that movement of infested commodities may contribute to the dissemination of L. serricorne, facilitating gene flow.

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Study of genetic variability of umbilical cord blood using RAPD assay

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v20i4.54144 ◽

2021 ◽

Vol 20 (4) ◽

pp. 848-854

Author(s):

Md Faruque Miah ◽

Md Shad Ebna Rahaman ◽

Sanjana Fatema Chowdhury ◽

Mohammad Golam Rob Mahmud

Keyword(s):

Genetic Variability ◽

Genetic Distance ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Gene Diversity ◽

Human Subjects ◽

Random Amplified Polymorphic Dna ◽

Medical Science ◽

Wiener Index

Background: The genetic variability of Umbilical Cord Blood (UCB) is most important for newborn screening, therapeutic possibility of haematological disorders as well as for the establishment of cord blood banking and stem cell research. Method: Genetic variability of umbilical cord blood (UCB) of 22 human subjects was evaluated first time by applying Random Amplified Polymorphic DNA (RAPD) assay using six decamar primers (B-14, OPB-05, OPB-08, OPB-12, OPB-19 and UBC-122). Result: A total number of bands were recorded 312 from 116 polymorphic loci and single monomorphic locus. All the markers showed highest polymorphism (100%) except the primer OPB 08 (92.31%) among tested individuals. The genetic distance was observed with highest 1.0 and lowest 0.72 respectively whereas mean genetic distance was recorded 0.90. Considering Shannon-Wiener index average diversity was recorded 0.139365. The mean Nei genetic similarity was found 0.17 which was found opposition to genetic distances. A phylogenetic relationship among the individual subjects was also observed between the linkage distances of 11 to 27 with 8 clades, 3 subclusters and a cluster. In addition, average allele frequency p and q was observed 0.08156 and 0.948751 respectively whereas highest intra locus gene diversity and average gene diversity were found 3.323817 and 0.144572 respectively. Conclusion: Considering different parameters, higher genetic variability was found among the experimental subjects, probably due to the mixture DNA of parents and newborn. Bangladesh Journal of Medical Science Vol.20(4) 2021 p.848-854

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Genetic variability and evolution of the Schistosoma genome analysed by using random amplified polymorphic DNA markers

Molecular and Biochemical Parasitology ◽

10.1016/0166-6851(93)90219-n ◽

1993 ◽

Vol 59 (2) ◽

pp. 211-221 ◽

Cited By ~ 60

Author(s):

Véronique Barral ◽

Patrice This ◽

Danièle Imbert-Establet ◽

Claude Combes ◽

Michel Delseny

Keyword(s):

Genetic Variability ◽

Dna Markers ◽

Random Amplified Polymorphic Dna

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Genetic variability of Indian yaks using random amplified polymorphic DNA markers

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb10.1842 ◽

2013 ◽

Vol 10 (43) ◽

pp. 8558-8561

Author(s):

P Ramesha K ◽

V Prasanna Kumar K ◽

Ch K ◽

rashekar ◽

S ◽

...

Keyword(s):

Genetic Variability ◽

Dna Markers ◽

Random Amplified Polymorphic Dna

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Genetic diversity of fruit borer, Helicoverpa armigera (Lepidoptera: Noctuidae) based on random amplified polymorphic DNA- polymerase chain reaction

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v39i2.20428 ◽

2014 ◽

Vol 39 (2) ◽

pp. 263-271

Author(s):

AKMZ Rahman ◽

MA Haque ◽

SN Alam ◽

P Yasodha ◽

V Balasubramani

Keyword(s):

Genetic Variability ◽

Helicoverpa Armigera ◽

Indian Population ◽

Random Amplified Polymorphic Dna ◽

Similarity Coefficient ◽

Taq Polymerase ◽

Agroecological Zones ◽

Pcr Products ◽

Helicoverpa Armígera ◽

Fruit Borer

The genetic variability of Helicoverpa armigera (Hübner) at different agroecological zones of Bangladesh in comparison with Indian population was conducted in India during September 2008 to February 2009. A total of 12 H. armigera populations of which 10 populations collected from different agroecological zones of Bangladesh and two populations from India were tested for their genetic variability. Eight out of the ten primers produced scorable PCR products by amplifying the template DNA with taq polymerase and were subjected for analysis. Those eight primers got amplified to a total of 138 markers which produced polymorphic markers. The similarity coefficient based on 138 RAPD markers ranged from 0.000 to 0.777 of the pair-wise combination among twelve samples of H. armigera. An UPGMA dendrogram based on Jaccards similarity coefficient was constructed for the 12 samples of H. armigera. The dendrogram showed that H. armigera population from Bangladesh had 25 to 45 percent similarity, and in its Indian population the similarity remained within this range. DOI: http://dx.doi.org/10.3329/bjar.v39i2.20428 Bangladesh J. Agril. Res. 39(2): 263-271, June 2014

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Using Random Amplified Polymorphic DNA to Assess Genetic Diversity and Structure of NaturalCalophyllum brasiliense(Clusiaceae) Populations in Riparian Forests

International Journal of Forestry Research ◽

10.1155/2014/305286 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8

Author(s):

Evânia Galvão Mendonça ◽

Anderson Marcos de Souza ◽

Fábio de Almeida Vieira ◽

Regiane Abjaud Estopa ◽

Cristiane Aparecida Fioravante Reis ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Random Amplified Polymorphic Dna ◽

Natural Populations ◽

Rio Grande ◽

Shannon Index ◽

High Genetic Diversity ◽

Family Structures ◽

Genetic Diversity And Structure ◽

Kinship Coefficients

The objective of this study was to assess the genetic variability in two natural populations ofCalophyllum brasilienselocated along two different rivers in the state of Minas Gerais, Brazil, using RAPD molecular markers. Eighty-two polymorphic fragments were amplified using 27 primers. The values obtained for Shannon index (I) were 0.513 and 0.530 for the populations located on the margins of the Rio Grande and Rio das Mortes, respectively, demonstrating the high genetic diversity in the studied populations. Nei’s genetic diversity (He) was 0.341 for the Rio Grande population and 0.357 for the Rio das Mortes population. These results were not significantly different between populations and suggest a large proportion of heterozygote individuals within both populations. AMOVA showed that 70.42% of the genetic variability is found within populations and 29.58% is found among populations (ФST=0.2958). The analysis of kinship coefficients detected the existence of family structures in both populations. Average kinship coefficients between neighboring individuals were 0.053 (P<0.001) in Rio das Mortes and 0.040 (P<0.001) in Rio Grande. This could be due to restricted pollen and seed dispersal and the history of anthropogenic disturbance in the area. These factors are likely to contribute to the relatedness observed among these genotypes.

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Occurrence and genetic variability of Candida parapsilosis sensu lato in Hungary

Journal of Medical Microbiology ◽

10.1099/jmm.0.46838-0 ◽

2007 ◽

Vol 56 (2) ◽

pp. 190-195 ◽

Cited By ~ 36

Author(s):

Sándor Kocsubé ◽

Mónika Tóth ◽

Csaba Vágvölgyi ◽

Ilona Dóczi ◽

Miklós Pesti ◽

...

Keyword(s):

Genetic Variability ◽

Candida Parapsilosis ◽

Sequence Data ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Its Region ◽

Molecular Data ◽

Rrna Gene ◽

Phenotypic Data ◽

Group I

The occurrence and genetic variability of Candida parapsilosis isolates in two Hungarian hospitals, located in Debrecen and Pécs, were examined. Among the 209 Candida isolates examined, 20 were found to belong to C. parapsilosis sensu lato, based on morphological, physiological and molecular data. The frequency of occurrence of C. parapsilosis isolates (9.6 %) was lower than that observed in Europe but higher than that observed previously in Hungary. The genetic variability of C. parapsilosis sensu lato isolates was also examined using random amplified polymorphic DNA (RAPD) analysis and sequence analysis of the intergenic transcribed spacer (ITS) region of the rRNA gene cluster. The genetic variability of the isolates was relatively high, as revealed by RAPD analysis. Two isolates were found to belong to the recently described Candida metapsilosis species (C. parapsilosis group III), based on ITS sequence data, RAPD analysis and phenotypic data. These two isolates could also be distinguished from C. parapsilosis sensu stricto isolates using a primer pair developed for the detection of C. parapsilosis group I isolates. To the best of the authors' knowledge, this is the first report on the identification of C. metapsilosis from bloodstream infection.

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