Genetic Relatedness of Pecan Cultivars Determined by Using Random Amplified Polymorphic DNA (RAPD)
Although pecan (Carya illinoinensis) is an economically important nut and timber crop, little is known about the nature of genetic variation among pecan cultivars. In addition, the pedigree of many cultivars remains unknown or is questionable. In this study, the genomic DNA of 20 pecan cultivars were analyzed by RAPD, using 20 randomly selected oligoes as primers. Based on their genetic similarities derived from the RAPD data, the 20 pecan cultivars were classified into different groups. Pecan cultivars within the same group displayed very little genetic variation, whereas cultivars in different groups showed significant diversity. The putative origins for some pecan cultivars previously believed to have unknown pedigrees were also identified based on the RAPD data obtained. Results of this study provide information useful for pecan cultivar classification and parent selection in pecan breeding programs.