(261) A PCR-based Linkage Map of Broccoli [Brassica oleracea(L.) var. italica] and Comparisons with Existing BrassicaMaps
A set of 216 PCR-based molecular markers was screened for polymorphisms using two morphologically dissimilar broccoli lines, `VI-158' and `BNC'. Fifty-nine of these markers, representing 69 detected polymorphisms and two morphological markers, were used to construct a genetic linkage map of broccoli [Brassicaoleracea (L.) var. italica] from a population of 162 F2:3 families generated from the cross between these two lines. Ten genetic linkage groups were generated that spanned a distance of 468 cM with an average interval width of 9.4 cm. This map represents the first combined SSR and SRAP map of Brassica oleracea. Comparisons are made to existing maps of Brassicanapus and to inter-specific maps of Brassicaoleracea. To our knowledge this is the first linkage map of broccoli [Brassicaoleracea (L.) var. italica] and should provide a useful tool for the genetic analysis of traits specific to the italica subspecies.