scholarly journals In vitro Propagation and Plantlet Regeneration from Doritaenopsis Purple Gem ‘Ching Hua’ Flower Explants

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1256-1258 ◽  
Author(s):  
Wagner A. Vendrame ◽  
Ian Maguire ◽  
Virginia S. Carvalho
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Naphthalene Acetic Acid ◽  
Shoot Induction ◽  
Flower Buds ◽  
Flower Stem ◽  
Normal Flower ◽  
Regenerated Plantlets ◽  
Lateral Flower

The effects of four types of explants removed from 10-cm flower stalks of Doritaenopsis Purple Gem ‘Ching Hua’ (immature apical flower buds, immature lateral flower buds, flower stem nodes, and flower pedicel sections) and combinations of two plant growth regulators [naphthalene acetic acid (NAA) and thidiazuron (TDZ)] on direct in-vitro shoot induction and multiplication were studied. Immature apical flower buds were the only explants that showed induction and multiplication of shoots in vitro. NAA at 5.4 and 10.7 μm combined with either 4.5 or 9.1 μm TDZ provided the fastest and greatest percentages of shoot induction (27% to 40%) and the greatest numbers of shoot multiplication (111–160 shoots per explant). In vitro–induced shoots were rooted on medium containing 5.4 μm NAA and developed into plantlets with normal vegetative and reproductive morphology. Regenerated plantlets were acclimatized, showing 100% survival and establishment in greenhouse. Plantlets were grown to maturity and showed normal flower morphology. No floral off-types were observed. The high rates of shoot multiplication obtained offer a means for mass clonal propagation of this and possibly other related Doritaenopsis hybrids.

scholarly journals A New Method for Rapid In Vitro Propagation of Apple and Pear

HortScience ◽  
2001 ◽  
Vol 36 (6) ◽  
pp. 1102-1106 ◽  
Author(s):  
V.R. Bommineni ◽  
H. Mathews ◽  
S.B. Samuel ◽  
M. Kramer ◽  
D.R. Wagner
Keyword(s):  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Induction Medium ◽  
Shoot Induction ◽  
Clonal Multiplication ◽  
Propagation Methods ◽  
Shoot Induction Medium ◽  
Tools And Techniques ◽  
Stem Slices

Improved in vitro clonal propagation methods are valuable tools for nurseries and growers, and are essential for manipulation and improvement of tree fruit germplasm using the tools and techniques of biotechnology. We have developed a rapid shoot multiplication procedure for clonal propagation of apple, Malus ×domestica cv. Gale Gala and pear, Pyrus communis L. cv. Bartlett. Rapid clonal multiplication was achieved after the following series of steps: pre-conditioning of micropropagated shoots, sectioning pre-treated stems into thin slices, placing slices onto shoot induction medium and incubating directly under cool-white fluorescent lights or after a brief dark incubation. Multiple induction of shoots recovered from stem slice explants within three weeks of culture. A maximum of 37% of cultured apple stem slices, and 97% of pear stem slices, showed induction of shoots. More shoots were recovered on phytagel solidified shoot induction medium than on agar. Cultured stem slices of both apple and pear showed maximum recovery of shoots from shoot induction medium supplemented with thidiazuron (TDZ) compared to medium supplemented with BAP and kinetin. Under ideal conditions, pear stems generated four times the shoots as the same quantity or length of apple shoots. Micropropagated shoots were rooted and transferred to the greenhouse and field nursery for further evaluation. Chemical names used: N-phenyl-N′-1,2,3-thidiazol-5-ylurea (thidiazuron or TDZ); 6-benzylaminopurine (BAP).

scholarly journals In vitro Micropropagation of Boswellia ovalifoliolata

2005 ◽  
Vol 60 (5-6) ◽  
pp. 505-507 ◽  
Author(s):  
Thummala Chandrasekhar ◽  
T. Mohammad Hussain ◽  
Boddu Jayanand
Keyword(s):  
Acetic Acid ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Naphthalene Acetic Acid ◽  
Nodal Explant ◽  
In Vitro Micropropagation ◽  
Boswellia Ovalifoliolata ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 μм) was conducted. The highest shoot multiplication (7.1 ± 0.2 shoots per node) was achieved in 50 d on MS supplemented with thidiazuron (2.72 μм). Excised shoot cuttings of 3.0 cm were placed on the MS basal medium supplemented with indole-3-acetic acid and indole-3-butyric acid alone and in combinations for rooting. Activated charcoal (100 mg l-1) and polyvinylpyrrolidone (40 mg l-1) were added to the medium to prevent browning of cultures. The regenerated plantlets have been successfully acclimatized and transferred to soil.

scholarly journals In vitro shoot multiplication of the biodiesel plant Jatropha curcas L. through direct organogenesis

2014 ◽  
Vol 49 (1) ◽  
pp. 41-46 ◽  
Author(s):  
MAKMS Hassan ◽  
N Begum ◽  
LS Bari ◽  
MAA Jahan
Keyword(s):  
Jatropha Curcas ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Jatropha Curcas L ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was established for in vitro shoot multiplication of the biodiesel plant, Jatropha curcas L. (Euphorbiaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 86.2% of nodal explants responded to produce maximum number (7.2 ± 0.68) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mg/l IAA. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v49i1.18854 Bangladesh J. Sci. Ind. Res. 49(1), 41-46, 2014

scholarly journals Influence of Cytokinins in Combination with GA3on Shoot Multiplication and Elongation of Tea Clone Iran 100 (Camellia sinensis(L.) O. Kuntze)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Reza Azadi Gonbad ◽  
Uma Rani Sinniah ◽  
Maheran Abdul Aziz ◽  
Rosfarizan Mohamad
Keyword(s):  
Camellia Sinensis ◽  
Woody Plants ◽  
Clonal Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Liquid Form ◽  
Solid Media

The use ofin vitroculture has been accepted as an efficient technique for clonal propagation of many woody plants. In the present research, we report the results of a number of experiments aimed at optimizing micropropagation protocol for tea (Camellia sinensis(L.) O. Kuntze) (clone Iran 100) using nodal segments as the explant. The effect of different combinations and concentrations of plant growth regulators (PGR) (BAP, TDZ, GA3) on shoot multiplication and elongation was assessed. The influence of exposure to IBA in liquid form prior to transfer to solid media on rooting of tea microshoots was investigated. The results of this study showed that the best treatment for nodal segment multiplication in terms of the number of shoot per explant and shoot elongation was obtained using 3 mg/L BAP in combination with 0.5 mg/L GA3. TDZ was found to be inappropriate for multiplication of tea clone Iran 100 as it resulted in hyperhydricity especially at concentrations higher than 0.05 mg/L. Healthy shoots treated with 300 mg/L IBA for 30 min followed by transfer to 1/2 strength MS medium devoid of PGR resulted in 72.3% of shoots producing roots and upon transferring them to acclimatization chamber 65% survival was obtained prior to field transfer.

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

scholarly journals An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.) 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 186-194 ◽  
Author(s):  
Jana Šedivá ◽  
Pavla Zahumenická ◽  
Eloy Fernández Cusimamani
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Growth Characteristics ◽  
Root Induction ◽  
Shoot Induction ◽  
Free Medium ◽  
In Vitro Production ◽  
Vitro Production

This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2–3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGR-free medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

scholarly journals Effect of Bavistin and Adenine Sulphate on In vitro Shoot Multiplication of Picrorhiza scrophulariiflora

1970 ◽  
Vol 19 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Pranay Bantawa ◽  
Olivia Saha Roy ◽  
Parthadeb Ghosh ◽  
Tapan Kumar Mondal
Keyword(s):  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Virgin Soil ◽  
Adenine Sulphate ◽  
Multiple Shoot Buds ◽  
Picrorhiza Scrophulariiflora ◽  
Regenerated Plantlets

An alternative protocol for in vitro propagation of Picrorhiza scrophulariiflora is described using bavistin and adenine sulphate. The explants differentiated into multiple shoot buds on MS supplemented with various concentrations of bavistin and adenine sulphate ranging from 0 - 400 mg/l either alone or in combination. Maximum number of multiple shoots were obtained on MS containing the combination of bavistin (100 mg/l) and adenine sulphate (100 mg/l). In this combination as high as 28 shoots per explant was achieved and also vetrification of the cultures were not recorded. This study also demonstrates that the bavistin has stronger cytokinin-like activity than adenine sulphate. For instance, it was observed that bavistin alone in the concentration of 300 mg/l produced as high as 24 shoots per explant, however, adenine sulphate (100 mg/l) could produce a maximum of 18 shoots per explant. Moreover, higher or lower concentration did not improve the shoot multiplication. The microshoots were separated from the multiple shoots and transferred to MS containing various concentrations of auxins. Among them, NAA (1 mg/l) produced as high as 6 roots per explant. The regenerated plantlets were hardened in plastic cups (6 x 8 cm) containing 9 : 1 virgin soil and soil at Kyongnosla nursery and acclimated for four weeks. A 90% survival rate of the plants was recorded after 60 days. D.O.I. 10.3329/ptcb.v19i2.5441 Plant Tissue Cult. & Biotech. 19(2): 237-245, 2009 (December)

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

scholarly journals Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 398-402 ◽  
Author(s):  
Mohammed Elsayed El-Mahrouk ◽  
Yaser Hassan Dewir ◽  
Yougasphree Naidoo
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Genetic Fidelity ◽  
Naphthalene Acetic Acid ◽  
Axillary Shoot ◽  
Ms Medium ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Protocol ◽  
Regenerated Plantlets

The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L−1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L−1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L−1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L−1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L−1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 TDZ and 1 mg·L−1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or full-strength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.

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