06-857, a Green Leaf Lettuce Breeding Line with Resistance to Leafminer and Lettuce Mosaic Virus

Seed certification and the use of cultivars containing one of two, probably allelic, recessive genes, mo11 and mo12, are the principal control methods for Lettuce mosaic virus (LMV) in lettuce. Although for a few LMV isolates, mo12 confers resistance with most isolates, the genes mo11 or mo12 confer a tolerance, and virus accumulation is readily detected in mo1-carrying plants. This phenotype complicates evaluation of the resistance status, in particular for mo11, for which there are no viral strains against which a true resistance is expressed. Two green fluorescent protein (GFP)-tagged viruses were constructed, derived from a non-resistance breaking isolate (LMV-0) and from a resistance-breaking isolate (LMV-E). An evaluation of 101 cultivars of known status was carried out with these recombinant viruses. Using the LMV-0-derived recombinant, identification of mo1-carrying cultivars was simple because, contrary to its wild-type parent, systemic movement of LMV-0-GFP was abolished in resistant plants. This assay detected four cases of misidentification of resistance status. In all these cases, further tests confirmed that the prior resistance status information was incorrect, so that a 100% correlation was observed between LMV-0-GFP behavior and the mo1 resistance status. Similarly, the LMV-E-derived recombinant allowed the identification of mo12 lettuce lines because its systemic movement was restricted in mo12 lines but not in susceptible or in mo11 lines. The tagged viruses were able to systemically invade another host, pea, irrespective of its resistance status against another member of the genus Potyvirus, Pea seed-borne mosaic virus. The use of these recombinant viruses could therefore greatly facilitate LMV resistance evaluation and speed up lettuce breeding programs.

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Red Leaf Lettuce Breeding Line with Resistance to Corky Root, 06-810

HortScience ◽

10.21273/hortsci.50.1.143 ◽

2015 ◽

Vol 50 (1) ◽

pp. 143-144

Author(s):

Beiquan Mou

Keyword(s):

Breeding Line ◽

Corky Root ◽

Lettuce Breeding ◽

Leaf Lettuce

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Green Leaf Lettuce Breeding Lines with Resistance to Corky Root, 06-831 and 06-833

HortScience ◽

10.21273/hortsci.46.9.1324 ◽

2011 ◽

Vol 46 (9) ◽

pp. 1324-1325 ◽

Cited By ~ 3

Author(s):

Beiquan Mou

Keyword(s):

Green Leaf ◽

Breeding Lines ◽

Corky Root ◽

Lettuce Breeding ◽

Leaf Lettuce

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Crisphead Lettuce Breeding Lines with Resistance to Corky Root and Lettuce Mosaic Virus

HortScience ◽

10.21273/hortsci.42.3.701 ◽

2007 ◽

Vol 42 (3) ◽

pp. 701-703 ◽

Cited By ~ 6

Author(s):

Beiquan Mou ◽

Ryan J. Hayes ◽

Edward J. Ryder

Keyword(s):

Mosaic Virus ◽

Lettuce Mosaic Virus ◽

Breeding Lines ◽

Corky Root ◽

Lettuce Breeding

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Novel pathotypes of lettuce mosaic virus — breakdown of a durable resistance?

Developments in Plant Pathology - Breeding for Disease Resistance ◽

10.1007/978-94-017-0954-5_14 ◽

1992 ◽

pp. 169-174 ◽

Cited By ~ 1

Author(s):

D. A. C. Pink ◽

H. Lot ◽

R. Johnson

Keyword(s):

Mosaic Virus ◽

Durable Resistance ◽

Lettuce Mosaic Virus

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Comparison between non-parametric indexes in the selection of biofortified curly lettuce

Comunicata Scientiae ◽

10.14295/cs.v11i.3351 ◽

2020 ◽

Vol 11 ◽

pp. e3351

Author(s):

Joicy Vitória Miranda Peixoto ◽

Gabriel Mascarenhas Maciel ◽

Ana Carolina Silva Siquieroli ◽

Lucas Medeiros Pereira ◽

José Magno Queiroz Luz ◽

...

Keyword(s):

Agronomic Traits ◽

Indirect Selection ◽

Stem Diameter ◽

Anthocyanin Content ◽

Grand Rapids ◽

Commercial Cultivars ◽

Lettuce Breeding ◽

Selection Indexes ◽

Leaf Lettuce ◽

Selection Of

Selection indexes can be applied to simultaneous analysis of traits, increasing the efficiency of breeding in choosing the genotypes that meet the needs of both the market and the consumer. While it is a technic applied to several cultures, there are few studies about it in lettuce lines. For this reason, the aim of this study was to comparate assess the efficiency of different selection indexes for selecting biofortifiedleaf lettuce lines with good agronomic traits. The experiment was carried out at the Estação Experimental de Hortaliças of Federal University of Uberlândia (UFU), campus Monte Carmelo, in 2018. The experiment was designed in full randomized blocks of 3 repetitions with 25 leaf lettuce genotypes (22 lines from cultivars Pira 72 versus Uberlândia 10000 and 3 commercial cultivars – cv. Grand Rapids, UFU-Biofort and Uberlândia 10000). The lines are part of the UFU biofortified lettuce breeding program and were assessed for the total green mass (g), the stem diameter (cm), commercial leaves count, plant diameter (cm), foliar temperature (°C), SPAD index, anthocyanin content (mg 100g-1 sample), and bolting (days after sowing). To estimate selection gains, 10 genotypes were selected using both direct and indirect selection, as well as the traditional index, the sum of ranks index, Willians base index, and the multiplicative index. Mulamba and Mock sum of ranks index and Subandi multiplicative index provided the biggest total gains for the traits assessed in biofortified leaf lettuce. Both indexes were similar in selecting genotypes.

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Avaliação da reação de genótipos de alface (Lactuca sativa L.) ao Lettuce mosaic virus (LMV)

Bragantia ◽

10.1590/s0006-87052007000100008 ◽

2007 ◽

Vol 66 (1) ◽

pp. 61-68 ◽

Cited By ~ 5

Author(s):

Rosa Maria Chung ◽

Joaquim Adelino de Azevedo Filho ◽

Addolorata Colariccio

Keyword(s):

Mosaic Virus ◽

Immunosorbent Assay ◽

Lactuca Sativa ◽

Enzyme Linked Immunosorbent Assay ◽

Lettuce Mosaic Virus ◽

Lactuca Sativa L

O trabalho teve como meta avaliar a reação de 18 linhagens superiores do programa de melhoramento de alface (Lactuca sativa L.) do IAC e de seis cultivares comerciais, ao Lettuce mosaic virus (LMV). Em condições de campo, na região de Atibaia (SP), foram observados sintomas de mosaico, nanismo e necrose em plantas das cultivares Rider, 'Karla H25' e Hortência. O vírus presente nos isolados foi identificado por meio de inoculação mecânica em plantas indicadoras e diferenciadoras e de testes sorológicos de Plate Trapped Antigen-Enzyme linked-immunosorbent assay (PTA-ELISA). Nas amostras avaliadas, identificou-se a espécie LMV pelo PTA-ELISA e do patotipo IV pela reação nas hospedeiras diferenciais. Para a avaliação do comportamento dos genótipos de alface, foi empregado o LMV isolado 'Karla H25'. Foram submetidos à inoculação 24 genótipos de alface empregando-se, como controle positivo, a alface 'White Boston' por sua suscetibilidade ao LMV. O delineamento experimental foi inteiramente ao acaso e analisado pelo teste do qui-quadrado. Detectaram-se genótipos com comportamento de suscetibilidade e de tolerância. Nos genótipos 3 e 4, foram observadas plantas com comportamento de tolerância ao LMV isolado 'Karla H25', enquanto nos demais genótipos, constataram-se plantas com comportamento suscetível. O plantio de cultivares tolerantes pode ser uma alternativa aos prejuízos causados pela infecção pelo LMV com conseqüente diminuição do uso de produtos químicos para o controle dos afídeos vetores.

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Growth analysis of green-leaf lettuce under different sources and doses of organic and mineral fertilization

Revista Colombiana de Ciencias Hortícolas ◽

10.17584/rcch.2019v13i2.8521 ◽

2019 ◽

Vol 13 (2) ◽

pp. 237-247

Author(s):

Rúbia Rejane Ribeiro ◽

Jose Luiz Rodrigues Torres ◽

Valdeci Orioli-Junior ◽

Hamilton Cesar De Oliveira Charlo ◽

Dinamar Márcia Da Silva Vieira

Keyword(s):

Organic Matter ◽

Growth Analysis ◽

Chicken Manure ◽

Residual Effects ◽

Green Leaf ◽

Organic Fertilization ◽

Mineral Fertilization ◽

Lettuce Yield ◽

Leaf Lettuce ◽

Different Sources

The objective of this study was to evaluate the influence of different sources and doses of organic and mineral fertilization on the production of green-leaf lettuce. The experiment design used randomized blocks in a factorial scheme (6×3), with six doses of fertilizers (1 = 0; 2 = 25; 3 = 50; 4 = 100; 5 = 150, and 6 = 200% of the recommended fertilization for green-leaf lettuce crop) and three sources of fertilizers [cattle manure (CaM) and chicken manure (ChM), decomposed, on a wet basis and applied 100% at planting at the doses: CaM – 0, 12.5, 25, 50, 75, 100 Mg ha-1; ChM – 0, 5, 10, 20, 30, 40 Mg ha-1; mineral fertilization (MF) varying the N levels: 0, 37.5, 75, 150, 225, 300 kg ha-1 plus 400 kg ha-1 of P2O5 and 60 kg ha-1 of K2O]. The fertilization with CaM and ChM was more efficient than the MF at increasing the production of green-leaf lettuce, mainly because of the higher residual effects of P in the Oxisol. The ChM provided a higher soil pH, P and K, while the CaM provided a higher soil Mg, organic carbon and organic matter. The dose with 144% of organic fertilization exclusively on a wet basis corresponding to 72 Mg ha-1 of CaM and 29 Mg ha-1 of ChM resulted in the highest green-leaf lettuce yield.

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