scholarly journals Isolation and Characterization of 13 Microsatellite Loci from Pedicularis rex (lousewort)

HortScience ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 1129-1131 ◽  
Author(s):  
Pan-Hui Huang ◽  
Wen-Bin Yu ◽  
Jun-Bo Yang ◽  
Hong Wang ◽  
Lu Lu
Keyword(s):  
Population Genetics ◽  
Microsatellite Markers ◽  
Endemic Species ◽  
Microsatellite Loci ◽  
Floral Variation ◽  
Isolation And Characterization ◽  
Horticultural Traits ◽  
Capture Technique

Pedicularis rex C. B. Clarke ex Maxim., an endemic species with potential horticultural traits from Himalaya, has a unique cup-like petiole structure and highly infraspecific floral variation among members of the lousewort genus (Orobanchaceae). We developed 13 microsatellite markers from three microsatellite-enriched libraries (AG, AC, and AAG) of P. rex with a modified biotin–streptavidin capture technique. Polymorphism of each locus was assessed in 22 individuals with representation of five populations of P. rex. Number of alleles per locus (A) ranged from two to seven with an average of 4.38. The observed and expected heterozygosities varied from 0.03 to 0.86 and 0.45 to 0.84, respectively. Additionally, among the 13 identified microsatellite markers, 11 of them were successfully amplified in species P. thamnophila, and five of them showed polymorphisms. This study may provide important information for further investigation on the population genetics, introduction, and acclimatization of P. rex and its congeners.

scholarly journals Isolation and Characterization of Microsatellite Loci for the Ornamental Plant Primula obconica Hance (Primulaceae)

HortScience ◽  
2010 ◽  
Vol 45 (2) ◽  
pp. 314-315 ◽  
Author(s):  
Hai-fei Yan ◽  
Xue-jun Ge ◽  
Chi-ming Hu ◽  
Gang Hao
Keyword(s):  
Genetic Variation ◽  
Genetic Structure ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Ornamental Plant ◽  
Microsatellite Variation ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Fiasco Protocol

Nine microsatellite loci were isolated from Primula obconica using the FIASCO protocol. We used 30 individuals from three populations for the assessment of microsatellite variation. Seven loci were detected with microsatellite polymorphism. The number of alleles per locus ranged from three to seven. The average observed heterozygosity and expected heterozygosity ranged from 0.167 to 0.6 and from 0.409 to 0.653, respectively. These microsatellite markers will be useful to assess the genetic variation and genetic structure of P. obconica.

Isolation and characterization of microsatellite markers forDipteronia dyerana(Sapindaceae), an endangered endemic species in China

2011 ◽  
Vol 98 (10) ◽  
pp. e271-e273 ◽  
Author(s):  
Chen Chen ◽  
Bing-Bing Ren ◽  
Xi-Hui Xu ◽  
Cheng-Xin Fu ◽  
Ying-Xiong Qiu
Keyword(s):  
Microsatellite Markers ◽  
Endemic Species ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

HortScience ◽  
2010 ◽  
Vol 45 (4) ◽  
pp. 690-692 ◽  
Author(s):  
Jiang-Chong Wu ◽  
Jing Yang ◽  
Zhi-Jian Gu ◽  
Yan-Ping Zhang
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Moringa Oleifera ◽  
Microsatellite Primers ◽  
Genetic Studies ◽  
Isolation And Characterization ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

scholarly journals Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites

Sociobiology ◽  
2017 ◽  
Vol 64 (3) ◽  
pp. 352 ◽  
Author(s):  
Yu-Lei Dang ◽  
Hong-Gui Zhang ◽  
Yu-Feng Meng ◽  
Min Zhang ◽  
Sha Zhao ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Subterranean Termites ◽  
Polymorphic Markers ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

We isolated 15 and 18 highly polymorphic genomic microsatellite markers from two subterranean termites, Reticulitermes aculabialis and R. labralis, respectively. A total of 53 alleles were detected in 15 microsatellite loci of R. aculabialis, and the alleles were 3.533±1.302 (mean±SD), while the corresponding data of R. labralis were 115 detected alleles in 18 microsatellite loci with 6.389±1.754 alleles. The observed and expected heterozygosity was 0.496±0.236 and 0.564±0.125 in R. aculabialis, and 0.368±0.263 and 0.702±0.115 in R. labralis, respectively. Seven loci were highly polymorphic (PIC>0.5) in R. aculabialis, and 15 loci were highly polymorphic (PIC>0.5) in R. labralis. All loci showed Hardy–Weinberg equilibrium. These polymorphic markers provide useful tools for population genetic and breeding system studies of subterranean termites.

scholarly journals Short Note: Isolation and Characterization of Microsatellite Loci in Castanopsis fissa in Lower Subtropical China

2010 ◽  
Vol 59 (1-6) ◽  
pp. 299-300 ◽  
Author(s):  
Lei Dong ◽  
Zheng-Feng Wang ◽  
Peng Zhu ◽  
Wan-Hui Ye
Keyword(s):  
Genetic Structure ◽  
Microsatellite Markers ◽  
Repetitive Dna ◽  
Microsatellite Loci ◽  
Spatial Genetic Structure ◽  
Short Note ◽  
Fine Scale ◽  
Subtropical China ◽  
Isolation And Characterization

Abstract We report on the development and characterization of ten microsatellite markers from repetitive DNA enriched libraries for Castanopsis fissa from lower subtropical China. The number of alleles ranged from three to thirteen. Observed and expected heterozygosities ranged from 0.265 to 0.818, and 0.270 to 0.873, respectively. These microsatellite markers will be used to study fine-scale spatial genetic structure of C. fissa in 20 ha Dinghushan plot in lower subtropical China.

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