scholarly journals Basal Salt Composition, Cytokinins, and Phenolic Binding Agents Influence In Vitro Growth and Ex Vitro Establishment of Magnolia ‘Ann’

HortScience ◽  
2012 ◽  
Vol 47 (11) ◽  
pp. 1625-1629 ◽  
Author(s):  
J. Kevin Parris ◽  
Darren H. Touchell ◽  
Thomas G. Ranney ◽  
Jeffrey Adelberg
Keyword(s):  
In Vitro Growth ◽  
Growth Responses ◽  
Ex Vitro ◽  
Salt Composition ◽  
Indolebutyric Acid ◽  
Binding Agents ◽  
Half Strength ◽  
Series Of Experiments ◽  
Basal Salts

In vitro growth responses of Magnolia ‘Ann’ to basal salt composition, cytokinins, and phenolic binding agents were investigated in a series of experiments to refine micropropagation protocols. Murashige and Skoog (MS), half-strength MS, Woody Plant Medium (WPM), Driver and Kuniyuki (DKW), and Blaydes basal salts in conjunction with 1 g·L−1 activated charcoal (AC) or 1 g·L−1 polyvinylpyrrolidone (PVP) were evaluated as multiplication media. Benzylaminopurine (BAP), meta-topolin (mT), or 6-(γ,γ-dimethylallylamino) purine (2iP) at 2, 4, or 8 μM was investigated to optimize the cytokinin concentration. Murashige and Skoog medium supplemented with 2 μM BAP with no phenolic binding agent was an optimal multiplication medium that yielded 3.2 ± 0.2 shoots with a mean length of 17.2 ± 1.8 mm over an 8-week period. For rooting, microshoots were cultured on half-strength MS media supplemented with 0, 5, 10, or 20 μM indolebutyric acid (IBA) with or without AC. Media containing AC produced elongated microshoots suitable for rooting and ex vitro establishment. Microshoots cultured on medium supplemented with AC also had higher in vitro rooting (16%) and higher ex vitro rooting (75%) compared with those without AC regardless of in vitro IBA concentration.

scholarly journals Micropropagation of Antelope Bitterbrush [Purshia tridentata (Pursh) DC.]

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 312-314 ◽  
Author(s):  
John L. Edson ◽  
David L. Wenny ◽  
Annette Leege-Brusven
Keyword(s):  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Axillary Shoots ◽  
Full Strength ◽  
Woody Plant Medium ◽  
Half Strength ◽  
In Vitro Survival

In vitro—derived microshoots of antelope bitterbrush, incubated for 1 month in media supplemented with 0.44 μm BA, grew 0.8 and 1.1 cm longer in woody plant medium (WPM) compared to full-strength and half-strength Murashige and Skoog (MS) media, respectively. Explants cultured in WPM supplemented with 0.44 μm BA and 0.54 μm NAA produced a mean of five axillary shoots per explant. Explants dipped in 0.1% IBA or 0.1% NAA rooted best in 0.1% IBA with 89% success ex vitro vs. 60% success in vitro. Survival of acclimatized plantlets rooted ex vitro was 95%, while 50% survived when rooted in vitro. After 1 year of greenhouse growth, 98% of plantlets survived and flowered. Chemical names used: benzyladenine (BA), 3-indolebutyric acid (IBA), 1-naphthaleneacetic acid (NAA).

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

scholarly journals Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)

2018 ◽  
Vol 48 (7) ◽  
Author(s):  
Renato Fernandes Galdiano Júnior ◽  
Cibele Mantovani ◽  
Eliana Gertrudes de Macedo Lemos
Keyword(s):  
Survival Data ◽  
Culture Medium ◽  
Seedling Survival ◽  
In Vitro Growth ◽  
Growth Data ◽  
Carbohydrate Source ◽  
Carbohydrate Supplementation ◽  
Percentage Survival ◽  
Half Strength

ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex vitro survival were lowest when the plantlets were grown in the absence of a carbohydrate source and highest (>90% survival) when supplemented with glucose. According to our findings, the addition of either glucose (30g L-1) or sucrose (30g L-1) is recommended for mass propagation of C. schilleriana.

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals Micropropagation of White Flowering Eastern Redbud (Cercis canadensis var. alba L.)

1990 ◽  
Vol 8 (4) ◽  
pp. 177-179
Author(s):  
S. Yusnita ◽  
R. L. Geneve ◽  
S. T. Kester
Keyword(s):  
Root System ◽  
Day Treatment ◽  
Shoot Multiplication ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
Cercis Canadensis ◽  
Half Strength ◽  
Eastern Redbud ◽  
Being Moved

Abstract A white flowering Eastern redbud (Cercis canadensis var. alba L.) has been successfully micropropagated. Two node explants collected from the initial flush of spring growth were cultured on woody plant medium (WPM). Increased shoot multiplication occurred at 10,15 and 20 μM (2.3, 3.4 and 4.5 ppm) benzyladenine (BA). Microshoots were rooted in vitro on half strength WPM with a 15-day treatment of 100 and 300 μM (18.6 and 55.9 ppm) α-naphthaleneacetic acid (NAA) or 100 and 300 μM (20.3 and 60.9 ppm) indolebutyric acid (IBA) prior to being moved to full strength WPM without growth regulators. Percentage rooting and the mean number of roots per cutting were comparable between NAA and IBA treated microcuttings, however, the subsequent root morphology differed between the two treatments. NAA treated plants developed a coarse, unbranched root system, while IBA treated cuttings developed a more desireable fine, branched root system. Rooted microshoots were successfully acclimated to greenhouse conditions.

Stimulatory effects of aluminum on in vitro growth ofFrankia

1999 ◽  
Vol 77 (9) ◽  
pp. 1321-1326 ◽  
Author(s):  
J M Igual ◽  
J O Dawson
Keyword(s):  
Total Protein ◽  
Acid Soils ◽  
Total Protein Content ◽  
In Vitro Growth ◽  
Experimental Medium ◽  
Binding Agents ◽  
Survival And Growth ◽  
Lactic Acids ◽  
Strain Dependent

To investigate the effects of aluminum (Al) on in vitro growth (total protein content) of Frankia, seven isolates were incubated for 25 days at five nominal Al concentrations (0-500 µM) at pH 4.8. The concentrations of monomeric Al ranged between 25.6 and 106.7 µM. The experimental medium was devoid of inorganic phosphate and Fe-EDTA to avoid Al precipitation. There was an Al-free control at pH 6.4 to assess the effects of low pH alone on Frankia growth. Growth estimated as total protein of all strains was notably increased at pH 6.4, but only Frankia CcI3 was able to grow in the Al-free medium at pH 4.8. Nominal Al concentrations from 125 to 500 µM enhanced the growth of all Frankia isolates. The Al response was strain dependent, but all strains were stimulated at the 500 µM (107 µM monomeric) Al concentration. The final pH of media decreased as Al concentrations increased. At the end of the experiment, the presence of citric, oxalic, malic, and lactic acids as possible Al binding agents in media at 500 µM Al was assayed by enzymatic techniques. However, none of them could be detected. These results suggest that the survival and growth of Frankia strains in acid soils that are characteristically high in Al are more likely than previously estimated from in vitro growth assays.Key words: acid soils, actinorhizal plants, aluminum, Frankia.

Influence of in vitro growth conditions on the photosynthesis and survival of Castanea sativa plantlets during ex vitro transfer

2014 ◽  
Vol 75 (3) ◽  
pp. 625-639 ◽  
Author(s):  
Patricia L. Sáez ◽  
León A. Bravo ◽  
Mirtha I. Latsague ◽  
Marcelo J. Toneatti ◽  
Rafael E. Coopman ◽  
...  
Keyword(s):  
Castanea Sativa ◽  
Growth Conditions ◽  
In Vitro Growth ◽  
Ex Vitro ◽  
Ex Vitro Transfer

Proliferation and harvesting of secondary protocorms as a novel means for improving propagation of terrestrial orchids

2014 ◽  
Vol 62 (7) ◽  
pp. 614 ◽  
Author(s):  
Betty Mauliya Bustam ◽  
Kingsley Dixon ◽  
Eric Bunn
Keyword(s):  
Early Stage ◽  
Naphthaleneacetic Acid ◽  
Novel Approach ◽  
Stage 4 ◽  
Terrestrial Orchids ◽  
Half Strength ◽  
Conservation Concern ◽  
Basal Salts ◽  
Proliferation In Vitro

This study investigated optimisation of media and primary-protocorm development stages to enhance secondary-protocorm production as a novel means for propagation of terrestrial orchids, including taxa of conservation concern. Seeds of Caladenia latifolia were germinated asymbiotically on ½-strength Murashige and Skoog (MS) medium fortified with 5% (v/v) coconut water. Resulting protocorms at 3, 5 and 7 weeks of growth were subcultured to protocorm-proliferation media treatments consisting of ½-strength MS basal-salts medium with 6-benzylaminopurine (BA) and α- naphthaleneacetic acid (NAA) singly or in combination. Conversion of seeds to primary protocorms was high (87–92%). The highest percentage of secondary-protocorm proliferation was 40.1%, using 5-week-old protocorms (early Stage 4 of protocorm development) as explants and cultured on ½-strength MS with a combination of 5 µM NAA + 2 µM BA. Half-strength MS media containing a single plant-growth regulator (BA or NAA) were substantially less effective (<10% protocorm proliferation). The present study has provided a novel approach to sequential protocorm production that will be of value particularly for threatened orchids with limited seed availability. Protocorm proliferation in vitro enables a renewable supply of protocorms with which to conduct propagation, cryostorage and pilot restoration programs.

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