Regulation of Glycolytic Metabolism in Fresh-cut Carrots under Low Oxygen Atmosphere

Carrot (Daucus carota L.) shreds were stored under a continuous flow of air or 0.5% and 2% O2 (balance N,) for 9 days at 5 and 15C. The resulting changes in respiration, levels of glycolytic intermediates, and activities of ATP: phosphofructo kinase (ATP-PFK), and PPi: phosphofructokinase (PPi-PFK) were monitored. Carrots under low O atmosphere exhibited an increase in RQ due to a greater reduction in 02 consumption than in CO2 production, and the increase in RQ was greater at 0.5% than at 2% O2 at both temperatures. Fructose 1,6-bisphosphate (F1,6P) accumulated with decreased O2 atmosphere and was 2-fold greater at 0.5% than at 2% O2 atmosphere at both temperatures. The levels of other glycolytic intermediates were not significantly influenced by low O2. The increase in PPi-PFK activity occurred at the same time as F1,6P accumulation. A similar relationship was not found with ATP-PFK. These results suggest that PPi-PFK may be involved in regulation of glycolysis under low O2 atmosphere.

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Effects of Low-oxygen Atmosphere on Ethanolic Fermentation in Fresh-cut Carrots

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.122.1.107 ◽

1997 ◽

Vol 122 (1) ◽

pp. 107-111 ◽

Cited By ~ 41

Author(s):

Hisashi Kato-Noguchi ◽

Alley E. Watada

Keyword(s):

Alcohol Dehydrogenase ◽

Daucus Carota ◽

Continuous Flow ◽

Pyruvate Decarboxylase ◽

Oxygen Atmosphere ◽

Low Oxygen ◽

Ethanolic Fermentation ◽

Fresh Cut ◽

Fermentation Metabolism

Carrot (Daucus carota L.) root shreds were stored under a continuous flow of 0.5% and 2% O2 (balance N2) or in air for 7 days at 5 and 15 °C to study the regulation of ethanolic fermentation metabolism. Low-O2 atmospheres of 0.5% and 2% caused increases in ethanol and acetaldehyde concentrations and the activities of alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC) compared to air. By day 3, ethanol increased 38-, 25-, 13-, and 9.5-fold; acetaldehyde increased 20-, 13-, 7.7-, and 5.6-fold; ADH increased 7.6-, 6.3-, 3.8-, and 2.7-fold; and PDC increased 4.2-, 3.9-, 2.3-, and 2.2-fold in samples at 0.5% O2 at 15 or 5 °C and at 2% O2 at 15 or 5 °C, respectively, compared with corresponding samples in air. These results indicate that ethanolic fermentation was accelerated more in the 0.5% than in the 2% O2 atmosphere and more at 15 °C than at 5 °C. The acceleration of ethanolic fermentation may allow production of some ATP, which may permit the carrot tissues to survive.

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Low-oxygen Atmosphere Increases Fructose 2,6-bisphosphate in Fresh-cut Carrots

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.121.2.307 ◽

1996 ◽

Vol 121 (2) ◽

pp. 307-309 ◽

Cited By ~ 7

Author(s):

Hisashi Kato-Noguchi ◽

Alley E. Watada

Keyword(s):

Daucus Carota ◽

Oxygen Atmosphere ◽

Low Oxygen ◽

Fresh Weight ◽

Fresh Cut ◽

Fructose 1,6 Bisphosphate

This study was undertaken to determine the effect of low-O2 atmosphere on the concentration of fructose 2,6-bisphosphate (Fru-2,6-P2), which can activate the enzyme pyrophosphate-dependent:phosphofructokinase (PPi-PFK) to catalyze the reaction from fructose 6-phosphate to fructose 1,6-bisphosphate (Fru-1,6-P2). Fru-2,6-P2 remained unchanged in carrot (Daucus carota L.) root shreds stored under air, but it increased 3.0- and 5.3-fold at 2% and 0.5% O2 atmosphere, respectively, at 5C, and the increases were almost twice as great at 15C. The concentration of PPi ranged from 17 to 33 nmol·g-1 fresh weight, which is more than sufficient for the PPi-PFK to proceed. Thus, low-O2 atmosphere appeared to hasten glycolysis of carrot shreds by increasing Fru-2,6-P2, which activated PPi-PFK toward glycolysis.

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EFFECTS OF LOW OXYGEN CONCENTRATIONS ON GLYCOLYSIS IN LIGHTLY PROCESSED CARROTS

HortScience ◽

10.21273/hortsci.30.2.189f ◽

1995 ◽

Vol 30 (2) ◽

pp. 189f-189

Author(s):

Hisashi Kato-Novuchi ◽

Alley E. Watada

Keyword(s):

Continuous Flow ◽

Oxygen Atmosphere ◽

The Other ◽

Low Oxygen ◽

Air Atmosphere ◽

Glycolytic Intermediates ◽

Oxygen Condition ◽

Most Significant Change ◽

Phosphofructokinase Activity ◽

Oxygen Concentrations

Carrot (Daucus carota L.) shreds were stored under a continuous flow of 0.5% O2 (balance N2), 2% O2 (balance N2), or air for 9 days at 5C and 15C. The resulting changes in respiration and levels of the glycolytic intermediates were monitored. Low-oxygen atmosphere reduced respiration rate, but the RQ increased. The RQ was higher at 0.5% O2 than at 2% O2 atmosphere at both 5C and 15C. The most significant change in the levels of glycolytic intermediates was the accumulation of fructose 1,6-biphosphate. The level of fructose 1,6-biphosphate at 0.5% O2 was about 2-fold greater than at 2% O2 atmosphere at both 5C and 15C. The level of the other glycolytic intermediates at low-oxygen atmosphere was similar to that held in air atmosphere. These results suggest that phosphofructokinase activity in the tissue of carrots may increase under low-oxygen condition and it may be one of the controlling points in the glycolytic pathway affected by low oxygen concentrations.

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RESPIRATION RATE AND RESPIRATORY QUOTIENT OF FRESH-CUT MANGO (MANGIFERA INDICA L.) IN LOW OXYGEN ATMOSPHERE

Acta Horticulturae ◽

10.17660/actahortic.2000.509.55 ◽

2000 ◽

pp. 471-478 ◽

Cited By ~ 10

Author(s):

N. Rattanapanone ◽

Alley E. Watada

Keyword(s):

Respiration Rate ◽

Respiratory Quotient ◽

Mangifera Indica ◽

Oxygen Atmosphere ◽

Low Oxygen ◽

Fresh Cut

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Ethanol content in cut roses at low oxygen atmosphere storage

Horticultural Science ◽

10.17221/4478-hortsci ◽

2012 ◽

Vol 29 (No. 4) ◽

pp. 148-152 ◽

Cited By ~ 1

Author(s):

J. Goliáš ◽

F. Kobza

Keyword(s):

Carbon Dioxide ◽

Cold Storage ◽

Oxygen Atmosphere ◽

Low Oxygen ◽

Trace Concentration ◽

Oxygen Stress ◽

Ethanol Content ◽

Air Ventilation ◽

Fresh Cut ◽

Atmosphere Storage

Low oxygen atmosphere is beneficial for preservation of fresh cut roses. Flower tolerance to specific atmosphere yielding anaerobic products was determined. A suitable gas mixture of ULO conditions (0.8% oxygen and 0.1% carbon dioxide) was shown to lead to elevated ethanol production in tissues. The tissue ethanol content in ULO and RA atmosphere (21% oxygen and 0.03% carbon dioxide) was 300 mg/l and 6 mg/l, respectively. After the exposure to ULO conditions when the material was in air ventilation under cold storage, ethanol decreased to the trace concentration comparable with that at the beginning of storage. The oxygen stress caused only small fluctuations in the content of acetaldehyde with difference from 3 to 6 mg/l. The presence of acetaldehyde in the pulp of cut roses indicated a negligible degree of injury by low oxygen. There were only minimal differences between ULO and RA in non-volatile compounds such as sucrose, glucose and fructose. Buds of cut roses did not open during the storage in ULO conditions and in the prolonged phase of air ventilation their opening was delayed for the next 15 days of cold storage.

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THE UNIQUE FETAL RED CELL AND ITS FUNCTION

PEDIATRICS ◽

10.1542/peds.51.3.494 ◽

1973 ◽

Vol 51 (3) ◽

pp. 494-500 ◽

Cited By ~ 2

Author(s):

Frank A. Oski

Keyword(s):

Red Blood Cells ◽

Newborn Infant ◽

Blood Cells ◽

Human Fetus ◽

Exchange Transfusion ◽

Oxygen Atmosphere ◽

Hypoxic Stress ◽

Red Cell ◽

Low Oxygen ◽

Rapid Replacement

The red blood cells of the human fetus differ in many major respects from the red cells of the normal adult. These differences appear admirably suited for the acquisition, transport, and release of oxygen in the low oxygen atmosphere of intrauterine existence. These same differences appear to confer a handicap to the cell in the extrauterine environment, particularly under conditions of hypoxic stress. The rapid replacement of these cells by artificial means, such as early exchange transfusion, may offer an advantage to the newborn infant in certain clinical situations.

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Modeling Wound-induced Respiration of Fresh-cut Carrots (Daucus carota L.)

Journal of Food Science ◽

10.1111/j.1365-2621.2003.tb05797.x ◽

2003 ◽

Vol 68 (9) ◽

pp. 2735-2740 ◽

Cited By ~ 43

Author(s):

B. B. SURJADINATA ◽

L. CISNEROS-ZEVALLOS

Keyword(s):

Daucus Carota ◽

Daucus Carota L ◽

Fresh Cut

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Triosephosphate metabolism by mature boar spermatozoa

Reproduction Fertility and Development ◽

10.1071/r97038 ◽

1997 ◽

Vol 9 (6) ◽

pp. 577 ◽

Cited By ~ 2

Author(s):

A. R. Jones

Keyword(s):

Glycolytic Pathway ◽

Dihydroxyacetone Phosphate ◽

Glycolytic Intermediates ◽

Boar Sperm ◽

Equilibrium Concentrations ◽

Fructose 1,6 Bisphosphate ◽

Boar Spermatozoa

Boar sperm rapidly interconverted dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, produced fructose-1,6-bisphosphate, approximately equilibrium concentrations of fructose 6-phosphate and glucose 6-phosphate but not glycerol or glycerol 3-phosphate. In the presence of 3-chloro-1-hydroxypropanone, an inhibitor of stage 2 of the glycolytic pathway, the triosephosphates were metabolized faster, produced less fructose-1,6-bisphosphate, fructose 6-phosphate and glucose 6-phosphate, but not glycerol or glycerol 3-phosphate. This suggests that these cells may have the capacity to convert glycolytic intermediates into a storage metabolite to conserve carbon atoms for the eventual synthesis of lactate.

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