scholarly journals Mapping a Wild Tomato Introgression Associated with Tomato Yellow Leaf Curl Virus Resistance in a Cultivated Tomato Line

2000 ◽  
Vol 125 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Peter M. Hanson ◽  
Dario Bernacchi ◽  
Sylvia Green ◽  
Steven D. Tanksley ◽  
Venkataramappa Muniyappa ◽  
...  
Keyword(s):  
Restriction Enzymes ◽  
Tomato Yellow Leaf ◽  
Rflp Markers ◽  
Yellow Leaf ◽  
Chromosome 11 ◽  
Wild Tomato ◽  
Tomato Line ◽  
And Control ◽  
Leaf Curl Virus ◽  
Leaf Curl

Tomato yellow leaf curl virus (TYLCV), a heterogeneous complex of whitefly-vectored geminiviruses, is a serious production constraint of tomato (Lycopersicon esculentum Mill.) in Asia, the Middle East, and the Americas. In this study we report on mapping of a DNA fragment introgressed into cultivated tomato presumably from the wild species L. hirsutum Humb. and Bonpl. and found to be associated with TYLCV resistance. To locate introgressions of wild tomato alleles in TYLCV-resistant tomato line H24, its DNA was digested with six restriction enzymes and probed with 90 RFLP markers evenly spaced throughout the genome. This polymorphism survey revealed the presence of one wild tomato introgression each on chromosomes 8 and 11. Plants of a F2 cross between H24 and a susceptible tomato line were probed with randomly amplified polymorphic DNA (RFLP) markers linked to the targeted regions and F3 families were developed by self-pollination of F2 plants that carried none, one, or both introgressions in either homozygous or heterozygous states. Plants of F3 families, parents, and control tomato line Ty52 (homozygous for the Ty-1 allele for TYLCV tolerance) were exposed to viruliferous whiteflies (Bemisia tabaci Gennadius) in greenhouses at the Asian Vegetable Research and Development Center, Taiwan, and the University of Agricultural Sciences, Bangalore, India. Results indicated that F3 families homozygous for the introgression on chromosome 11 were resistant to TYLCV at both locations. Additional probing showed that the chromosome 11 introgression spanned markers TG36 to TG393, covering a distance of at least 14.6 centimorgans. This is the first report of TYLCV resistance in tomato mapped to chromosome 11.

scholarly journals Whitefly Resistance Traits Derived from the Wild Tomato Solanum pimpinellifolium Affect the Preference and Feeding Behavior of Bemisia tabaci and Reduce the Spread of Tomato yellow leaf curl virus

2011 ◽  
Vol 101 (10) ◽  
pp. 1191-1201 ◽  
Author(s):  
M. J. Rodríguez-López ◽  
E. Garzo ◽  
J. P. Bonani ◽  
A. Fereres ◽  
R. Fernández-Muñoz ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Glandular Trichomes ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Tomato Cultivar ◽  
Solanum Pimpinellifolium ◽  
Wild Tomato ◽  
Type Iv ◽  
Leaf Curl Virus ◽  
Leaf Curl

Breeding of tomato genotypes that limit whitefly (Bemisia tabaci) access and feeding might reduce the spread of Tomato yellow leaf curl virus (TYLCV), a begomovirus (genus Begomovirus, family Geminiviridae) that is the causal agent of tomato yellow leaf curl disease. TYLCV is restricted to the phloem and is transmitted in a persistent manner by B. tabaci. The tomato breeding line ABL 14-8 was developed by introgressing type IV leaf glandular trichomes and secretion of acylsucroses from the wild tomato Solanum pimpinellifolium accession TO-937 into the genetic background of the whitefly- and virus-susceptible tomato cultivar Moneymaker. Results of preference bioassays with ABL 14-8 versus Moneymaker indicated that presence of type IV glandular trichomes and the production of acylsucrose deterred the landing and settling of B. tabaci on ABL 14-8. Moreover, electrical penetration graph studies indicated that B. tabaci adults spent more time in nonprobing activities and showed a reduced ability to start probing. Such behavior resulted in a reduced ability to reach the phloem. The superficial type of resistance observed in ABL 14-8 against B. tabaci probing significantly reduced primary and secondary spread of TYLCV.

scholarly journals Identification of Tomato yellow leaf curl virus Naturally Infecting Common Bean in Japan

Plant Disease ◽  
2014 ◽  
Vol 98 (10) ◽  
pp. 1447-1447 ◽  
Author(s):  
M. S. Shahid ◽  
K. T. Natsuaki
Keyword(s):  
Common Bean ◽  
Natural Infection ◽  
Rolling Circle Amplification ◽  
Restriction Enzymes ◽  
Tomato Yellow Leaf ◽  
Whole Genome Sequence ◽  
Yellow Leaf ◽  
Sequence Identity ◽  
Leaf Curl Virus ◽  
Leaf Curl

Tomato yellow leaf curl virus (TYLCV; family Geminiviridae, genus Begomovirus) is a devastating pathogen vectored by the whitefly Bemisia tabaci causing significant yield losses to tomato crops in Japan since 1998 (2). So far, there has been no report of this virus infecting common bean (Phaseolus vulgaris; family Fabaceae) in Japan. But recently, TYLCV has been reported from P. vulgaris in Spain and China (1,3). P. vulgaris is a vegetable crop commonly grown during spring to summer in Japan. In the course of a study to assess virus incidence on P. vulgaris exhibiting yellowing and thickening symptoms with 20 to 30% incidence, four symptomatic and two healthy samples were collected in August, 2012 from a single mix-cropping field (where P. vulgaris was cultivated together with tomato [Solanum lycopercisum] crop and a high B. tabaci population density was observed on plants) in Komae, Japan. Meanwhile, the vector for one crop can transmit the disease to the next crop very easily. To identify possible begomovirus present in symptomatic P. vulgaris plants, total nucleic acids were extracted from plants with and without symptoms using PhytoPure Plant DNA Extration Kit (GE Amersham Biosciences, UK) and begomovirus replication was confirmed from symptomatic leaves in 3 out of 4 plants when hybridized with a specific non-radioactive probe to the coat protein region of TYLCV (5) using a Biotin DNA Labelling Kit (Fermentas). To confirm the identity of the virus detected, leaf samples were further tested by PCR using the TYLCV specific detection primers (TYF/TYR) corresponding to the V2/CP region and a DNA fragment of an expected size was obtained from the samples positive for TYLCV in Southern hybridizations (4). The PCR products were sequenced directly and these sequences showed the highest nucleotide sequence identity >99% to the TYLCV isolate AB116630 reported from Japan. Rolling circle amplification (RCA) was done on all samples to produce concatamers and with Sac I restriction enzyme an amplicon of ~2.7 kb was obtained and cloned into PUC118 vector (Takara, Japan). Multiple clones were obtained showing the same restriction pattern with different restriction enzymes, and three of them were randomly selected and sequenced by Macrogen (Japan). Sequence analysis showed all three clones were identical and one sequence (J18.11) was submitted to GenBank. This sequence (GenBank Accession No. KJ585666) was 2,774 nt long and displayed the arrangement of four ORFs (AC1, AC2, AC3, and AC4 in complementary sense) and two ORFs (AV2 and AV1) on virion sense) typical of the genome of a begomovirus. This whole genome sequence was compared with those of other reported begomoviruses and exhibited greater than 99% nt sequence identity to a previously reported TYLC sequence (JN183876). Efforts to identify the presence of an additional begomovirus components or DNA satellites either by PCR and/or RCA were negative, suggesting that this is a monopartite begomovirus and an isolate of TYLCV. This is the first report of a natural infection of TYLCV in P. vulgaris in Japan. The presence of TYLCV on P. vulgaris could therefore represent a serious threat for this valuable crop in Japan. Thus, it is important to develop an effective way to control this virus to reduce the further losses. References: (1) Y. H. Ji et al. Plant Dis. 96:1229, 2012. (2) K. Kato et al. Ann. Phytopathol. Soc. Jpn. 64:552, 1998. (3) J. Navas-Castillo et al. Plant Dis. 83:29, 1999. (4) M. S. Shahid et al. J. Phytopahol. 161:205, 2013. (5) M. S. Shahid et al. Viruses 6:189, 2014.

scholarly journals Toward Fine Mapping of the Tomato Yellow Leaf Curl Virus Resistance Gene Ty-2 on Chromosome 11 of Tomato

HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 614-618 ◽  
Author(s):  
Yuanfu Ji ◽  
John W. Scott ◽  
David J. Schuster
Keyword(s):  
Resistance Genes ◽  
Dominant Gene ◽  
Tomato Yellow Leaf ◽  
Resistance Locus ◽  
Yellow Leaf ◽  
Chromosome 11 ◽  
Race 2 ◽  
Molecular Marker Analysis ◽  
Leaf Curl Virus ◽  
Leaf Curl

The whitefly-transmitted Tomato yellow leaf curl virus (TYLCV) is a major pathogen of tomatoes grown in tropical and subtropical regions of the world. Several genes of different origins conferring resistance to TYLCV have been introgressed to the cultivated tomato (Solanum lycopersicum), including the single dominant gene, Ty-2, that originated from S. habrochiates and was previously mapped to a 19-cM region on the long arm of chromosome 11 delimited by restriction fragment length polymorphism markers TG36 and TG393. In the present study, we confirmed the dominant inheritance of the Ty-2 gene from TYLCV evaluation and molecular marker analysis of an F2 segregating population derived from a commercial hybrid that carries the Ty-2 gene. Evaluating recombinants recovered from the F2 progeny for TYLCV resistance localized the Ty-2 gene to a marker interval of 5.5 cM between C2_At1g07960 (82.5 cM) and C2_At4g32930 (88 cM). Additional recombinants were identified for the target region carrying the Ty-2 gene. TYLCV evaluation of the progeny from these recombinants further delimited the Ty-2 gene to a 4.5-cM interval between C2_At1g07960 (82.5 cM) and cLEN-11-F24 (87 cM). The smaller introgressions no longer include the fusarium wilt race 2 resistance locus (I-2), which should facilitate combining the two resistance genes in cis configuration. The polymerase chain reaction-based markers developed from the present study can be used to precisely monitor the introgression of the Ty-2 gene, thus offering the opportunity to pyramid TYLCV resistance genes from different sources as well as resistance genes for other pathogens into elite tomato cultivars.

Control of the Bemisia tabaci/Tomato yellow leaf curl virus complex on protected tomato crops in Algarve (Portugal)*

EPPO Bulletin ◽  
2002 ◽  
Vol 32 (1) ◽  
pp. 31-35
Author(s):  
A. F. Arsenio ◽  
E. Neto ◽  
N. Ramos ◽  
S. Mangerico ◽  
E. Fortunato ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Virus Complex ◽  
Leaf Curl Virus ◽  
Leaf Curl

Study of a collection of tomato hybrids with genetic resistance to the yellow leaf curl virus in Southern Russia

2020 ◽  
pp. 30-34
Author(s):  
С.Ф. Гавриш ◽  
Т.А. Редичкина ◽  
А.В. Буц ◽  
Г.М. Артемьева
Keyword(s):  
Resistance Genes ◽  
Molecular Genetic Analysis ◽  
Tomato Yellow Leaf ◽  
Tomato Mosaic Virus ◽  
Average Weight ◽  
Yellow Leaf ◽  
The South ◽  
Economically Valuable Traits ◽  
Leaf Curl Virus ◽  
Leaf Curl

Дана информация об изучении коллекции гибридов F1томата (Solanum lycopersicum L.) зарубежной селекции различных фирм-оригинаторов, рекомендованных производителями семян как толерантные к вирусу желтой курчавости листьев томата. Все гибриды обладали комплексом хозяйственно ценных признаков и набором генов устойчивости к основным заболеваниям томата, в том числе к новому для юга России опасному патогену с максимальным потенциальным риском – вирусу желтой курчавости листьев томата (Tomato yellow leaf curl virus — TYLCV). Исследования проведены в 2017-2018 годах в лаборатории пасленовых культур ООО «НИИСОК» и в лаборатории молекулярной диагностики растений ООО «Семеновод». Всего было протестировано 34 гибрида F1 томата. Гибриды оценивали по совокупности хозяйственно ценных признаков, также проводили молекулярно-генетический анализ на наличие и аллельное состояние основных генов устойчивости: к вирусу табачной мозаики (Tm2а), фузариозному увяданию (I2), вертициллезному увяданию (Ve), к кладоспориозу (Cf9), нематодам (Mi1.2), вирусу бронзовости томата (Sw5), вирусу желтой курчавости листьев томата (Ty3a). Установлено, что все проанализированные гибриды томата с заявленной оригинаторами семян устойчивостью к вирусу желтой курчавости листьев были гетерозиготны по гену Ty3a. На основании проведенных исследований и с учетом требований рынка разработаны модели гибридов F1 томата юга России. Перспективный гибрид томата должен обладать индетерминантным типом роста с укороченными междоузлиями (4,5-5 см) а также хорошей облиственностью. Плоды томата должны быть с красной равномерной окраской без зеленого пятна у плодоножки, с плоскоокруглой или округлой формой плода и со средней массой 220-270 г. Для повышения транспортабельности томатов необходимо, чтобы плоды отличались высокой прочностью и характеризовались хорошей лежкостью. Урожайность гибрида томата должна быть более 30 кг/м2, а товарность - не менее 85%. Гибрид томата должен обладать следующим набором генов устойчивости в гетерозиготном состоянии: Ty3a, Mi1.2, Cf-9, а также в гомозиготном состоянии: Tm2a, I2, Ve. The article provides information on the study of the collection of F1 tomato hybrids (Solanum lycopersicumL.) of foreign breeding from various firms-originators recommended for cultivation in regions with a strong spread of tomato yellow leaf curl virus. All hybrids had a complex of economically valuable traits and a set of genes for resistance to the main diseases of tomato, including a new dangerous pathogen for the South of Russia with a maximum potential risk — the tomato yellow leaf curl virus (TYLCV). The studies were carried out in 2017-2018 in the Solanaceae Laboratory of LLC NIISOK and in the Molecular Diagnostics Laboratory of Plants of LLC Semenovod. A total of 34 F1 tomato hybrids were tested. The hybrids were assessed by a set of economically valuable traits. Molecular genetic analysis was also carried out for the presence and allelic state of the main resistance genes: Tomato mosaic virus (Tm2a), Fusarium wilt (I2), Werticillium wilt (Ve), Cladosporium fulvum (Cf9), Nematodes (Mi1.2), Tomato spotted wilt virus (Sw5), Tomato yellow leaf curl virus (Ty3a). It was found that all the analyzed tomato hybrids with the declared by seed originators resistance to yellow leaf curl virus were heterozygous for the Ty3a gene. Based on the conducted research and taking into account the market requirements, models of F1 tomato hybrids for protected ground for the South of Russia have been developed. A promising tomato hybrid should have an indeterminate growth type with shortened internodes (4.5-5 cm) and good foliage. Tomato fruits should have a uniform red color without green shoulders, with a flat-round or round shape of the fruit and with an average weight of 220-270 g. To increase the transportability of tomatoes, it is necessary that the fruits are highly firm and characterized by good shelf life. The yield of tomato hybrid should be more than 30 kg/m2, and marketability should be at least 85%. The tomato hybrid should have the following set of resistance genes in a heterozygous state: Ty3a, Mi1.2, Cf-9, and also in a homozygous state: Tm2a, I2, Ve.

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