scholarly journals Comparison of Protein Profile and Peroxidases in Bush and Vine-type Tropical Pumpkin

2008 ◽  
Vol 133 (3) ◽  
pp. 315-319 ◽  
Author(s):  
Tao Wu ◽  
Jiashu Cao
Keyword(s):  
Peroxidase Activity ◽  
Single Gene ◽  
Protein Profile ◽  
Cucurbita Moschata ◽  
Protein Profiles ◽  
Relative Expression ◽  
Peroxidase Isozymes ◽  
Total Peroxidase Activity ◽  
Leaf Tissues ◽  
Root Tissues

Comparisons of total peroxidase activity and peroxidase isozymes as well as protein profiles among segregating, near-isogenic bush and vine phenotypes of pumpkin (Cucurbita moschata Duchesne) were investigated. Peroxidase activities of internode and leaf tissues of the bush plants were higher than those of respective vine tissues. Roots of bush plants, however, had a lower peroxidase activity than vine plants. In both bush and vine plants, peroxidase activities were lower in leaf tissues than in root and internode tissues. Electrophoretic comparisons revealed qualitative differences in peroxidase patterns in internodes between bush and vine plants. Moreover, qualitative differences between internode and root profiles were found between bush and vine plants in C. moschata. In conclusion, the results of this report revealed that a single gene conferring the bush phenotype in C. moschata might affect the relative expression of peroxidase activity, peroxidase isozymes, and protein profiles in leaf, internode, and root tissues.

Peroxidase activity and relative mobility at anthesis in flax genotrophs and their F2 progeny: developmental and genetic effects

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 495-504 ◽  
Author(s):  
M. A. Fieldes ◽  
J. Ross
Keyword(s):  
Genetic Control ◽  
Peroxidase Activity ◽  
Stem Elongation ◽  
Genetic Regulation ◽  
Relative Mobility ◽  
Activity Data ◽  
Peroxidase Isozymes ◽  
Developmental Age ◽  
Total Peroxidase Activity ◽  
Flax Genotrophs

The genetic regulation of the environmentally induced heritable difference in peroxidase activity between Durrant's large (L) and small (S) flax genotrophs was examined in leaves from plants ranging in developmental age from 6 days before anthesis to 3 days after. Mean peroxidase activity was higher for S than L and intermediate for the reciprocal F2's from L × S and S × L crosses (F2L × S and F2S × L). However, activity increased with development and, since there were small but significant differences in the average developmental ages of L, S, F2L × S, and F2S × L plants, the effects of development on activity had to be taken into account in examining the F2 activity data for segregation. A regression method was used to remove developmental effects and, underlying these effects, total peroxidase activity appeared to be regulated by a single locus with two alleles and L dominance. Two other dimorphic loci, both described previously, were also examined. One regulates the presence-absence of septa hairs in the seed capsules and the other the relative mobility of anionic peroxidase isozymes. There was no phenotypic linkage between the three segregating parameters. The genetic control of activity appeared to regulate cationic rather than anionic activity. In addition, a relationship between activity and plant height indicated either that peroxidase activity is one of the factors regulating main stem elongation or that the locus regulating peroxidase activity is linked to one of the loci involved in the regulation of plant height.Key words: flax genotrophs, peroxidase, genetic control, development.

Enhanced peroxidase activity associated with the hypersensitive response of Solanum dulcamara to the gall mite Aceria cladophthirus (Acari: Eriophyoidea)

1991 ◽  
Vol 69 (10) ◽  
pp. 2192-2196 ◽  
Author(s):  
R. Bronner ◽  
E. Westphal ◽  
F. Dreger
Keyword(s):  
Peroxidase Activity ◽  
Electrophoretic Pattern ◽  
Solanum Dulcamara ◽  
Pathogenesis Related Proteins ◽  
Peroxidase Isozymes ◽  
Pathogenesis Related ◽  
Total Peroxidase Activity ◽  
Gall Mite ◽  
Plant Pathogenesis ◽  
Related Proteins

Peroxidase activity and its electrophoretic pattern were studied in resistant Solanum dulcamara leaves following infestation by the gall mite Aceria cladophthirus. Total peroxidase activity increased in infested leaves and was related to enhancement of three major peroxidase isozymes. The highest activity was found for the intercellular peroxidases. The extraction procedures used suggest that these peroxidases are pathogenesis-related proteins. These results are discussed with reference to the lack of lignin barrier around the mite-induced necrotic local lesions. Key words: Aceria cladophthirus, gall mite, Solanum dulcamara, resistant plant, pathogenesis-related proteins, peroxidases isozymes.

scholarly journals PEROXIDASE ACTIVITY DIFFERS IN SWEET CORN GENOTYPES

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 434c-434
Author(s):  
J.K. Collins ◽  
C. Biles ◽  
E.V. Wann ◽  
P. Perkins-Veazie
Keyword(s):  
Peroxidase Activity ◽  
Sweet Corn ◽  
Storage Proteins ◽  
Storage Duration ◽  
Native Page ◽  
Banding Patterns ◽  
Peroxidase Isozyme ◽  
Peroxidase Isozymes ◽  
Total Peroxidase Activity ◽  
And Storage

Increased peroxidase activity is used to predict development of off-flavor in frozen sweet corn. However, peroxidase activity was not indicative of flavor changes in frozen supersweet (sh2) or sugar enhanced (sul/se) sweet corn genotypes. These results suggested an inactivation or absence of certain peroxidase isozymes. Frozen `Florida Staysweet' (sh2), `Merit' (sul), and `Bodacious' (sul/se) kernels were cut from cobs after 0 and 12 months of storage. Proteins extracted from acetone powders were separated by isoelectric focusing (IEF) and Native-PAGE. Banding patterns differed according to cultivar and storage duration. All cultivars contained a peroxidase isozyme having a molecular weight of 99 kD and pI of 4.5. The sul/se and su2 cultivars expressed an additional peroxidase band of 17.9 kD. An additional peroxidase isozyme (pI 5.0) appeared after 12 months of storage in the sul cultivar. This isozyme did not appear in sul/se or sh2 and is a possible marker for predicting off-flavor in corn. This isozyme may also catalyze off-flavor reactions in sul corn genotypes. Although changes in total peroxidase activity may not predict flavor loss in all genotypes, certain peroxidase isozymes may be useful in predicting and catalyzing off-flavor reactions in sul corn cultivars.

scholarly journals A Method for Prediction of Graft Incompatibility in Sweet Cherry

2012 ◽  
Vol 40 (1) ◽  
pp. 243 ◽  
Author(s):  
S. Filiz GUCLU ◽  
Fatma KOYUNCU
Keyword(s):  
Peroxidase Activity ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Ground Tissue ◽  
Tissue Samples ◽  
Total Peroxidase Activity ◽  
Graft Incompatibility ◽  
Old Trees

This study was carried out on 1-year old trees of ‘0900 Ziraat’ variety grafted onto ‘Kuş kirazi’, ‘Kara idris’, ‘Sari idris’, ‘MaxMa 14’, ‘MaxMa 60’ and ‘Gisela 5’ in order to determine their compatibility. For this purpose, total peroxidase activity was determined by spectrophotomery assay. Taking ground tissue samples were planned three times as before the grafting (beginning), and then 8 and 12 months after grafting. Total peroxidase activities of rootstocks before grafting varied between 10.80 ΔAg.min. (‘Kuş kirazi’) and 7.83 ΔAg.min. (‘Kara idris’) and were found to be statistically important. The peroxidase activity of ‘0900 Ziraat’ was 11.07ΔAg.min. and the closest value occurred in Prunus avium rootstock. The most different values occurred in rootstocks of ‘Kara idris’ and ‘Gisela 5’. The results showed that peroxidase activity increased in rootstock and graft scion. This increase had higher values in heterogenetic combinations especially in ‘0900 Ziraat’/‘Gisela 5’ and ‘0900 Ziraat’/‘Kara idris’ grafts. Peroxidase activity was decreased at the 12th month for all combinations. The highest value was obtained from ‘0900 Ziraat’/‘MaxMa 14’ combinaion with 29.17 ΔAg.min. while lowest one was ‘0900 Ziraat’/‘Kuş kirazi’ with 17.39. The findings showed that peroxidase activity could be used as a parameter in early determination of graft incompatibility.

The protein composition of gluten extracted from different wheats

1963 ◽  
Vol 3 (9) ◽  
pp. 85 ◽  
Author(s):  
JW Lee ◽  
CW Wrigley
Keyword(s):  
Carboxymethyl Cellulose ◽  
Protein Profile ◽  
Protein Composition ◽  
Tetraploid Species ◽  
Protein Profiles ◽  
Polyacrylamide Gels ◽  
Wheat Varieties ◽  
Gluten Proteins ◽  
Baking Quality ◽  
Triticum Species

The gluten proteins from eight commercial wheat varieties and four tetraploid Triticum species were separated by chromatograph on carboxymethyl-cellulose and by electrophoresis on polyacrylamide gels. There are obvious differences in the protein profiles of many of the commercial wheat varieties. Furthermore, the tetraploid species differ in many respects from the T. vulgare varieties. The possible relationship between baking quality and protein profile is discussed.

Diversification within grapevine cultivars goes through chimeric states

Genome ◽  
2004 ◽  
Vol 47 (3) ◽  
pp. 579-589 ◽  
Author(s):  
S Hocquigny ◽  
F Pelsy ◽  
V Dumas ◽  
S Kindt ◽  
M-C Heloir ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Diploid Species ◽  
Leaf Tissue ◽  
Pinot Noir ◽  
Vitis Vinifera L ◽  
Cell Layers ◽  
Self Fertilization ◽  
Leaf Tissues ◽  
Root Tissues ◽  
L1 And L2

Vitis vinifera 'Pinot' clones were analysed at 50 microsatellite loci to assess intravarietal genetic diversity. When analysing leaf tissue DNAs, polymorphism mainly resulted from the appearance of a third allele when two were expected for heterozygous loci in a diploid species. The sequencing of the three microsatellite alleles at two loci has confirmed their simultaneous presence in the leaf tissues. A hypothesis explaining the triallelic profiles at a locus is the presence of a periclinal chimera meristem structure, in which genetically different cell layers coexist. The periclinal chimeric state of two Vitis vinifera 'Pinot gris' clones was confirmed by splitting and analysing the genotypes resulting from L1 and L2 cell layers in progeny derived from self-fertilization, in root tissues, and in plants regenerated from somatic embryogenesis. Prevalence of chimerism in polymorphic clones observed in a collection of 145 accessions belonging to 'Pinot gris', 'Pinot noir', Pinot blanc', 'Pinot meunier', and 'Pinot moure' cultivars was demonstrated. The accumulation of somatic mutations and cell layer rearrangements allowed us to deduce the relationships between the various genotypes and to open a way for understanding the diversification process and the phylogeny in the 'Pinot' group.Key words: microsatellite, diversity, somatic mutation, chimerism, Vitis vinifera L., 'Pinot'.

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