A change of total peroxidase activity as a stress indicator in tomato Lycopersicum esculentum Mill cv. Arietta leaves

2000 ◽  
Vol 28 (5) ◽  
pp. A401-A401
Author(s):  
M. Donko ◽  
V. Abram
Keyword(s):  
Peroxidase Activity ◽  
Lycopersicum Esculentum ◽  
Stress Indicator ◽  
Total Peroxidase Activity

scholarly journals A Method for Prediction of Graft Incompatibility in Sweet Cherry

2012 ◽  
Vol 40 (1) ◽  
pp. 243 ◽  
Author(s):  
S. Filiz GUCLU ◽  
Fatma KOYUNCU
Keyword(s):  
Peroxidase Activity ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Ground Tissue ◽  
Tissue Samples ◽  
Total Peroxidase Activity ◽  
Graft Incompatibility ◽  
Old Trees

This study was carried out on 1-year old trees of ‘0900 Ziraat’ variety grafted onto ‘Kuş kirazi’, ‘Kara idris’, ‘Sari idris’, ‘MaxMa 14’, ‘MaxMa 60’ and ‘Gisela 5’ in order to determine their compatibility. For this purpose, total peroxidase activity was determined by spectrophotomery assay. Taking ground tissue samples were planned three times as before the grafting (beginning), and then 8 and 12 months after grafting. Total peroxidase activities of rootstocks before grafting varied between 10.80 ΔAg.min. (‘Kuş kirazi’) and 7.83 ΔAg.min. (‘Kara idris’) and were found to be statistically important. The peroxidase activity of ‘0900 Ziraat’ was 11.07ΔAg.min. and the closest value occurred in Prunus avium rootstock. The most different values occurred in rootstocks of ‘Kara idris’ and ‘Gisela 5’. The results showed that peroxidase activity increased in rootstock and graft scion. This increase had higher values in heterogenetic combinations especially in ‘0900 Ziraat’/‘Gisela 5’ and ‘0900 Ziraat’/‘Kara idris’ grafts. Peroxidase activity was decreased at the 12th month for all combinations. The highest value was obtained from ‘0900 Ziraat’/‘MaxMa 14’ combinaion with 29.17 ΔAg.min. while lowest one was ‘0900 Ziraat’/‘Kuş kirazi’ with 17.39. The findings showed that peroxidase activity could be used as a parameter in early determination of graft incompatibility.

Overexpression of the endogenous peroxidase-like gene spi 2 in transgenic Norway spruce plants results in increased total peroxidase activity and reduced growth

2001 ◽  
Vol 20 (7) ◽  
pp. 596-603 ◽  
Author(s):  
M. Elfstrand ◽  
C. Fossdal ◽  
F. Sitbon ◽  
O. Olsson ◽  
A. Lönneborg ◽  
...  
Keyword(s):  
Norway Spruce ◽  
Peroxidase Activity ◽  
Total Peroxidase Activity ◽  
Endogenous Peroxidase

Peroxidase activity and relative mobility at anthesis in flax genotrophs and their F2 progeny: developmental and genetic effects

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 495-504 ◽  
Author(s):  
M. A. Fieldes ◽  
J. Ross
Keyword(s):  
Genetic Control ◽  
Peroxidase Activity ◽  
Stem Elongation ◽  
Genetic Regulation ◽  
Relative Mobility ◽  
Activity Data ◽  
Peroxidase Isozymes ◽  
Developmental Age ◽  
Total Peroxidase Activity ◽  
Flax Genotrophs

The genetic regulation of the environmentally induced heritable difference in peroxidase activity between Durrant's large (L) and small (S) flax genotrophs was examined in leaves from plants ranging in developmental age from 6 days before anthesis to 3 days after. Mean peroxidase activity was higher for S than L and intermediate for the reciprocal F2's from L × S and S × L crosses (F2L × S and F2S × L). However, activity increased with development and, since there were small but significant differences in the average developmental ages of L, S, F2L × S, and F2S × L plants, the effects of development on activity had to be taken into account in examining the F2 activity data for segregation. A regression method was used to remove developmental effects and, underlying these effects, total peroxidase activity appeared to be regulated by a single locus with two alleles and L dominance. Two other dimorphic loci, both described previously, were also examined. One regulates the presence-absence of septa hairs in the seed capsules and the other the relative mobility of anionic peroxidase isozymes. There was no phenotypic linkage between the three segregating parameters. The genetic control of activity appeared to regulate cationic rather than anionic activity. In addition, a relationship between activity and plant height indicated either that peroxidase activity is one of the factors regulating main stem elongation or that the locus regulating peroxidase activity is linked to one of the loci involved in the regulation of plant height.Key words: flax genotrophs, peroxidase, genetic control, development.

Enhanced peroxidase activity associated with the hypersensitive response of Solanum dulcamara to the gall mite Aceria cladophthirus (Acari: Eriophyoidea)

1991 ◽  
Vol 69 (10) ◽  
pp. 2192-2196 ◽  
Author(s):  
R. Bronner ◽  
E. Westphal ◽  
F. Dreger
Keyword(s):  
Peroxidase Activity ◽  
Electrophoretic Pattern ◽  
Solanum Dulcamara ◽  
Pathogenesis Related Proteins ◽  
Peroxidase Isozymes ◽  
Pathogenesis Related ◽  
Total Peroxidase Activity ◽  
Gall Mite ◽  
Plant Pathogenesis ◽  
Related Proteins

Peroxidase activity and its electrophoretic pattern were studied in resistant Solanum dulcamara leaves following infestation by the gall mite Aceria cladophthirus. Total peroxidase activity increased in infested leaves and was related to enhancement of three major peroxidase isozymes. The highest activity was found for the intercellular peroxidases. The extraction procedures used suggest that these peroxidases are pathogenesis-related proteins. These results are discussed with reference to the lack of lignin barrier around the mite-induced necrotic local lesions. Key words: Aceria cladophthirus, gall mite, Solanum dulcamara, resistant plant, pathogenesis-related proteins, peroxidases isozymes.

Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail millet with differential salt tolerance

Plant Science ◽  
1999 ◽  
Vol 141 (1) ◽  
pp. 1-9 ◽  
Author(s):  
N Sreenivasulu ◽  
S Ramanjulu ◽  
K Ramachandra-Kini ◽  
H.S Prakash ◽  
H Shekar-Shetty ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Peroxidase Activity ◽  
Total Peroxidase Activity ◽  
Peroxidase Isoforms

Changes in Peroxidase in Isolated Aleurone Layers in Response to Gibberellic Acid

1983 ◽  
Vol 10 (1) ◽  
pp. 87 ◽  
Author(s):  
F Gubler ◽  
AE Ashford
Keyword(s):  
Gibberellic Acid ◽  
Peroxidase Activity ◽  
Time Course ◽  
Disc Electrophoresis ◽  
Peroxidase Isoenzymes ◽  
Total Peroxidase Activity ◽  
High Level

The effect of gibberellic acid (GA3) on peroxidase activity and isoenzyme complement was followed in isolated aleurone layers of barley (Hordeurn vulgare L.) over a 48-h period. A high level of peroxidase activity was initially present in freshly isolated aleurone layers. Disc electrophoresis resolved nine peroxidase isoenzymes. By 36 h incubation, total peroxidase activity had doubled in response to GA3 and two new peroxidase isoenzymes were observed. Peroxidase release from isolated aleurone layers was also greatly stimulated by GA3 and more isoenzymes were found in the medium. The time course of release was similar to that for α-amylase. Peroxidase activity in endosperm of whole germinating grains began to increase after 2 days, peaked at 4 days and subsequently declined.

scholarly journals Comparison of Protein Profile and Peroxidases in Bush and Vine-type Tropical Pumpkin

2008 ◽  
Vol 133 (3) ◽  
pp. 315-319 ◽  
Author(s):  
Tao Wu ◽  
Jiashu Cao
Keyword(s):  
Peroxidase Activity ◽  
Single Gene ◽  
Protein Profile ◽  
Cucurbita Moschata ◽  
Protein Profiles ◽  
Relative Expression ◽  
Peroxidase Isozymes ◽  
Total Peroxidase Activity ◽  
Leaf Tissues ◽  
Root Tissues

Comparisons of total peroxidase activity and peroxidase isozymes as well as protein profiles among segregating, near-isogenic bush and vine phenotypes of pumpkin (Cucurbita moschata Duchesne) were investigated. Peroxidase activities of internode and leaf tissues of the bush plants were higher than those of respective vine tissues. Roots of bush plants, however, had a lower peroxidase activity than vine plants. In both bush and vine plants, peroxidase activities were lower in leaf tissues than in root and internode tissues. Electrophoretic comparisons revealed qualitative differences in peroxidase patterns in internodes between bush and vine plants. Moreover, qualitative differences between internode and root profiles were found between bush and vine plants in C. moschata. In conclusion, the results of this report revealed that a single gene conferring the bush phenotype in C. moschata might affect the relative expression of peroxidase activity, peroxidase isozymes, and protein profiles in leaf, internode, and root tissues.

scholarly journals Peroxidase Activity, Isoenzymes, and Tissue Localization in Developing Highbush Blueberry Fruit

1991 ◽  
Vol 116 (5) ◽  
pp. 827-830 ◽  
Author(s):  
T.J. Miesle ◽  
A. Proctor ◽  
L.M. Lagrimini
Keyword(s):  
Enzyme Activity ◽  
Peroxidase Activity ◽  
Cell Walls ◽  
Vaccinium Corymbosum ◽  
Weight Basis ◽  
Highbush Blueberry ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Total Peroxidase Activity ◽  
Highbush Blueberries

The activity, isoenzymes, and histochemical location of peroxidase were studied in developing highbush blueberries (Vaccinium corymbosum L.). Total peroxidase activity increased during development when expressed on a fresh-weight basis, reaching a maximum in red berries and then declining. When peroxidase activity was expressed per berry it did not decline after the red-berry stage. Most of the enzyme was ionically bound to cell walls throughout development, with the number of isoenzymes increasing with maturity. Histochemical localization of peroxidase showed that enzyme activity spread throughout the berry during development.

TOTAL PEROXIDASE ACTIVITY IN TOMATOES RIN, MONEYMAKER AND THEIR F1 HYBRID

1981 ◽  
pp. 75-82 ◽  
Author(s):  
N. Gorin ◽  
F. T. Heidema
Keyword(s):  
Peroxidase Activity ◽  
F1 Hybrid ◽  
Total Peroxidase Activity
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