scholarly journals A Method for Prediction of Graft Incompatibility in Sweet Cherry

2012 ◽  
Vol 40 (1) ◽  
pp. 243 ◽  
Author(s):  
S. Filiz GUCLU ◽  
Fatma KOYUNCU

This study was carried out on 1-year old trees of ‘0900 Ziraat’ variety grafted onto ‘Kuş kirazi’, ‘Kara idris’, ‘Sari idris’, ‘MaxMa 14’, ‘MaxMa 60’ and ‘Gisela 5’ in order to determine their compatibility. For this purpose, total peroxidase activity was determined by spectrophotomery assay. Taking ground tissue samples were planned three times as before the grafting (beginning), and then 8 and 12 months after grafting. Total peroxidase activities of rootstocks before grafting varied between 10.80 ΔAg.min. (‘Kuş kirazi’) and 7.83 ΔAg.min. (‘Kara idris’) and were found to be statistically important. The peroxidase activity of ‘0900 Ziraat’ was 11.07ΔAg.min. and the closest value occurred in Prunus avium rootstock. The most different values occurred in rootstocks of ‘Kara idris’ and ‘Gisela 5’. The results showed that peroxidase activity increased in rootstock and graft scion. This increase had higher values in heterogenetic combinations especially in ‘0900 Ziraat’/‘Gisela 5’ and ‘0900 Ziraat’/‘Kara idris’ grafts. Peroxidase activity was decreased at the 12th month for all combinations. The highest value was obtained from ‘0900 Ziraat’/‘MaxMa 14’ combinaion with 29.17 ΔAg.min. while lowest one was ‘0900 Ziraat’/‘Kuş kirazi’ with 17.39. The findings showed that peroxidase activity could be used as a parameter in early determination of graft incompatibility.

Genetika ◽  
2013 ◽  
Vol 45 (3) ◽  
pp. 939-952 ◽  
Author(s):  
Sanja Radicevic ◽  
Sladjana Maric ◽  
Radosav Cerovic ◽  
Milena Djordjevic

The paper presents results of a three-year study of self-(in)compatibility in four economically important sweet cherry genotypes - ?Karina?, ?Kordia?, ?Regina? and ?Summit?, under agro-environmental conditions of Western Serbia. Determination of S-RNase genotype, microscopic observation of the pollen tube growth rate and assaying of the fruit set level after self-pollination were used to assess the genotypes. ?Kordia? (S3S6), ?Regina? (S1S3) and ?Summit? (S1S2) are self-incompatible genotypes, with a considerable number of pollen tubes ending the growth in the middle third of the style and lack of fruit set. ?Karina?, as S3S4 genotype, behaved as self-compatible, since its pollen tubes reached the base of the style and ovary, penetrating the nucellus. In addition, fruit set for ?Karina? was recorded in all three years of study (40.26%, 18.79% and 21.81%, respectively).


2010 ◽  
Vol 58 (4) ◽  
pp. 377-384 ◽  
Author(s):  
Z. Békefi ◽  
S. Vaughan ◽  
K. Tobutt

The sweet cherry (Prunus avium L.) gene-bank collection in Hungary comprises mainly local cultivars. The incompatibility (S) genotypes of 48 accessions from the central region of Hungary were investigated by PCR amplification of the intron regions of the SRNase and SFB genes responsible for compatibility relationships in sweet cherry. The Sgenotypes of 38 accessions were completely determined; they showed various pairs of nine alleles and could be assigned to 15 of the existing incompatibility groups or, in the case of three accessions having the novel genotype S6S13, to the new incompatibility group XLII. For 10 accessions only one S-allele could be identified, as a single S-RNase product was generated and the intron region of the SFB gene of the second allele could not be amplified.


Author(s):  
Tabita Teodora LISANDRU ◽  
Adrian FUSTOS ◽  
Viorel MITRE ◽  
Adelina DUMITRAS

The study present the detail descriptions of the stages of stone fruits growth, Prunus avium and Prunus persica, according to the BBCH scale. Phenological data were collected from 4 years old trees of P.avium and P. persica. The development stage of each organ was recorded during two growing seasons (2015-2016). Photographs of each stage were taken to illustrate the phenological growth stages. An accurate understanding of these stages is important for the correct timing of orchard management, as well as for disease and pest management, irrigation, flower thinning, fertilizer effectiveness etc.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Matej Vosnjak ◽  
Helena Sircelj ◽  
Metka Hudina ◽  
Valentina Usenik

AbstractThe aim of the present study was to evaluate the effect of post-flowering chilling of sweet cherry (Prunus avium L.) on the content of biochemical parameters in the leaf (chloroplast pigments, sugars and phenolics). The effect of chilling was investigated in two experiments. Potted 2-year-old trees of cv. 'Grace Star' and 'Schneiders' were exposed to one, two or three consecutive overnight chillings at an average air temperature of 4.7 °C (Experiment I), but in the following year only trees of 'Grace Star' were chilled at 2.2 °C (Experiment II), 3 to 7 weeks after flowering. The analysis of the biochemical parameters was performed by high performance liquid chromatography combined with electrospray ionization mass spectrometry. Chilling at 4.7 °C caused little or no stress, while 2.2 °C induced more intense stress with increased zeaxanthin, sugar and phenolic content in leaves, while exposure of trees to higher temperatures and closer to flowering showed no changes. Two or three consecutive overnight chilling periods increased the phenolic content and enhanced the accumulation of zeaxanthin in the leaves. Sucrose, sorbitol, fructose, total sugar, and total flavonoid content in leaves increased within 48 h after chilling. Zeaxanthin epoxidized within 24 h after one and 48 h after one and two consecutive overnight chillings.


HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 666g-667
Author(s):  
Charlotte M. Guimond ◽  
Preston K. Andrews ◽  
Gregory A. Lang

Young sweet cherry (Prunus avium) trees are typically upright, vegetatively vigorous, and nonprecocious, taking 5 to 6 years to come into production. To produce fruit in high-density orchards by year 3 or 4, development of lateral shoots for potential fruiting is critical in year 2 or 3. An experiment was designed to promote lateral branching on 2-year-old trees. The experiment was conducted in a commercial orchard in Roosevelt, Wash., with `Bing' and `Van' on the vigorous rootstocks Mazzard and Colt. The trees were planted at 415 trees per acre with three scaffolds trained into a “V” canopy design. The experimental variables were treatments with and without Promalin (1.8% BAP plus 1.8% GA4+7), applied at a ratio of 1:3 in latex paint at green tip stage; superimposed on these treatments were either heading cuts of each scaffold to 2 m long (or tipping the scaffold if it was <2 m), removing four to five buds subtending the terminal bud, a combination of heading and bud removal, or controls. On trees that were not treated with Promalin, three additional treatments included either removing subtending buds at budbreak, or removing buds at multiple locations along the scaffold at green tip or at budbreak. New lateral shoots were counted 4 weeks after budbreak, and the quality of the shoots (shoot diameter and angle of emergence) was measured at the time of summer pruning. Interactions between Promalin, bud manipulation, and pruning will be discussed in relation to development of canopy structure.


Author(s):  
Zs. Békefi ◽  
K. R. Tobutt ◽  
T. Sonneveld

Sweet cherries (Prunus avium L.) are generally self-incompatible and pollinator cultivars are needed in orchards for reliable yield. In Hungary, choosing the appropriate cross-compatible cultivar pairs has so far been based on traditional test-crosses in the field. In recent years PCR-based methods that allow the identification of the S-alleles responsible for compatibility have been elaborated. We have determined the S-allele constitution of 24 cultivars and four selections important to Hungarian growers and breeders using PCR-based methods developed at Horticulture Research International, East Malling. The 28 accessions had various pairs of 9 alleles including one new allele, Sr. They could be assigned to 12 of the existing incompatibility groups or to a new group (S4S12) for which the designation 'Group XXVII' is proposed. The cultivars `Krupnoplodnaja' and 'Rita' had novel genotypes, S5S9 and S5Sx, respectively and can be placed into group 0 that holds universal pollen donors. The genotype of the cultivar ‘Hedelfingeni óriás' grown in Hungary was found to be S3S4 and therefore different from the cultivar `Hedelfingen' that is widespread in Western Europe.


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