scholarly journals Genetic Analysis of Walnut Cultivars in China Using Fluorescent Amplified Fragment Length Polymorphism

2011 ◽  
Vol 136 (6) ◽  
pp. 422-428 ◽  
Author(s):  
Qingguo Ma ◽  
Junpei Zhang ◽  
Dong Pei
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Juglans Regia ◽  
Amplified Fragment Length Polymorphism ◽  
Polymorphic Marker ◽  
Similarity Coefficient ◽  
Group Method ◽  
Discrimination Power

Informative DNA fingerprints from 50 walnut cultivars (Juglans regia) in China were generated using amplified fragment length polymorphism (AFLP) markers to reveal their genetic diversity and relationships. Nine primer combinations were selected from 64 EcoR I/Mse I primer combinations to amplify the accessions. An average of 132 polymorphic loci per primer set was detected from the nine primer combinations. The discrimination power of each polymorphic marker (estimated by the polymorphism information content) ranged from 0.00 to 0.37 with an average of 0.19. A moderate level of genetic diversity was observed among the 50 cultivars. Their expected heterozygosity varied from 0.38 to 0.50 (average, 0.44), and Dice's similarity coefficient ranged from 0.53 to 0.86 (average, 0.70). The cluster analysis conducted using the unweighted pair group method of arithmetic averages method showed that all of the cultivars fell into five groups at Dice's similarity coefficient of 0.68. According to the comprehensive analyses based on the specific loci, similarity coefficient, and clustering results, six cultivars (Liaoning 1, Zixin, Shanhe 4, Zha 343, Tulare, and Chandler) were considered important germplasms of walnut cultivars.

scholarly journals Genetic Relationships among Native Species and Hybrid Cultivars of Asian Dendrobium (Orchidaceae) Using Amplified Fragment Length Polymorphism Markers

HortScience ◽  
2011 ◽  
Vol 46 (2) ◽  
pp. 192-196 ◽  
Author(s):  
Gen-Fa Zhu ◽  
Dong-Mei Li
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Native Species ◽  
Fragment Length Polymorphism ◽  
Genetic Relationships ◽  
Amplified Fragment Length Polymorphism ◽  
Similarity Coefficient ◽  
Group Method ◽  
Plant Materials ◽  
Arithmetic Average

This study addresses the phylogenetic relationships among native species and hybrid cultivars of Asian Dendrobium by amplified fragment length polymorphism (AFLP). The plant materials of this study are composed of 37 accessions belonging to native species in China and 63 accessions proposed to be hybrid cultivars originating from Japan and Korea. Eight AFLP primer combinations produced a total of 1658 fragments with an average of 207 fragments per primer pair, of which 1655 bands were polymorphic. Specific AFLP markers were identified in 29 of 100 tested Dendrobium accessions. Unweighted pair group method based on arithmetic average (UPGMA) analysis was performed on Dice's similarity coefficient matrix and also average similarity of each species and cultivar. The tested 100 Asian Dendrobium accessions were grouped into seven clusters with the similarity coefficient of 0.49. A first cluster consisted of 63 hybrid cultivars, 17 species of section Dendrobium, one species of section Formosae, and one species of section Callista. A second, fourth, and seventh cluster included five, three, and two species of section Dendrobium, respectively. A third group comprised five species of section Formosae. A fifth and sixth cluster contained three and two species of section Callista, respectively. These results indicated that the genetic relationships among tested Asian Dendrobium accessions were related to their origins, morphological classification, flower color, and pedigree, to some extent.

scholarly journals Genetic Diversity of Chinese Bayberry (Myrica rubra Sieb. et Zucc.) Accessions Revealed by Amplified Fragment Length Polymorphism

HortScience ◽  
2009 ◽  
Vol 44 (2) ◽  
pp. 487-491 ◽  
Author(s):  
Shuiming Zhang ◽  
Zhongshan Gao ◽  
Changjie Xu ◽  
Kunsong Chen ◽  
Guoyun Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Southern China ◽  
Genetic Relationships ◽  
Amplified Fragment Length Polymorphism ◽  
Group Method ◽  
Chinese Bayberry ◽  
Myrica Rubra

Amplified fragment length polymorphism (AFLP) was used to analyze genetic diversity of 100 accessions of Chinese bayberry (Myrica rubra Sieb. et Zucc.), one of the widely cultivated fruit tree crops in southern China. Six E-NN/M-NNN primer combinations were selected and a total of 236 bands were obtained, of which 177 were polymorphic (75.01%). An unweighted pair-group method of the arithmetic averages (UPGMA) was used to analyze the genetic relationships. The Dice's similarity coefficient among the Chinese bayberry accessions ranged from 0.75 to 1.00 and was 0.49 between Chinese bayberry and wax myrtle (M. cerifera L.). The 100 accessions of Chinese bayberry were clustered into two groups and seven subgroups. Subgrouping of Chinese bayberry was not related to the sex of the plant and color or size of the ripe fruit, but to some extent the region where the accession originated. However, the accessions from the same region did not necessarily belong to the same group or subgroup, which suggested the presence of extensive gene flow among different regions. Furthermore, close relationships between some morphologically similar accessions were found.

scholarly journals Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae)

2005 ◽  
Vol 28 (2) ◽  
pp. 267-270 ◽  
Author(s):  
Michelle Mantovani Gonçalves ◽  
Manoel Victor Franco Lemos ◽  
Pedro Manoel Galetti Junior ◽  
Patrícia Domingues de Freitas ◽  
Manuel Antonio Andrade Furtado Neto
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Litopenaeus Vannamei ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism

Genetic diversity of fringed brome (Bromus ciliatus) as determined by amplified fragment length polymorphism

2005 ◽  
Vol 83 (10) ◽  
pp. 1322-1328 ◽  
Author(s):  
Yong-Bi Fu ◽  
Bruce E. Coulman ◽  
Yasas S.N. Ferdinandez ◽  
Jacques Cayouette ◽  
Paul M. Peterson
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Habitat Restoration ◽  
Fragment Length Polymorphism ◽  
Natural Populations ◽  
Germplasm Conservation ◽  
Amplified Fragment Length Polymorphism ◽  
Large Area ◽  
Geographic Origins

Fringed brome ( Bromus ciliatus L.) is found in native stands throughout a large area of North America. Little is known about the genetic diversity of this species. The amplified fragment length polymorphism (AFLP) technique was applied to assess the genetic diversity of 16 fringed brome populations sampled in Canada from the provinces of Alberta, British Columbia, Quebec, and Saskatchewan. Four AFLP primer pairs were employed to screen 82 samples with four to six samples per population and 83 polymorphic AFLP bands scored for each sample. The frequencies of the scored bands in all assayed samples ranged from 0.01 to 0.99 and averaged 0.53. Analysis of molecular variance revealed that 52.6% of the total AFLP variation resided among the 16 populations and 20.6% among the four provinces. The five Quebec populations appeared to be genetically the most diverse and distinct. The AFLP variability observed was significantly associated with the geographic origins of the fringed brome populations. These findings are useful for sampling fringed brome germplasm from natural populations for germplasm conservation and should facilitate the development of genetically diverse regional cultivars for habitat restoration and revegetation.

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