Combined Effect of Hermetic Bag and Insect Resistant Variety for the Control of Larger Grain Borer and Maize Weevil in Stored Maize

Although Catalpa bungei is a forest plant with considerable economic and ornamental value in China, its wood and decorative qualities are constrained by insect pests such as the shoot borer Omphisa plagialis (Lepidoptera). Overexpressing insect resistance genes such as crystal genes to develop an insect-resistant variety of C. bungei is an environmental and ecological approach. However, genotype limitations and low regeneration rates of embryogenic calli (EC) inhibit the development of transformation and the insect-resistant gene expression system in C. bungei. Here, we first established embryogenic callus induction and regeneration systems of five genotypes using mature seed and stem segment explants; the highest induction and regeneration rates of EC were 39.89 and 100%, respectively. Next, an efficient and stable Agrobacterium-mediated genetic transformation system was developed from EC and its positive frequency was up to 92.31%. Finally, using the transformation system, 15 and 22 transgenic C. bungei lines that expressed Cry2A and Cry9Aa-like were generated, respectively. These transgenic lines that exhibited significantly higher resistance to O. plagialis in the laboratory and field have great promise for meeting the challenge of future pest management under changing climatic conditions. Additionally, this efficient, fast, and stable transformation system could be a potential tool for gene function analysis and forest tree genetic improvement.

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Evaluation of Sequential Sampling Plans for the Larger Grain Borer (Coleoptera: Bostrichidae) and the Maize Weevil (Coleoptera: Curculionidae) and of Visual Grain Assessment in West Africa

Journal of Economic Entomology ◽

10.1603/0022-0493-93.6.1822 ◽

2000 ◽

Vol 93 (6) ◽

pp. 1822-1831 ◽

Cited By ~ 7

Author(s):

W. G. Meikle ◽

N. Holst ◽

P. Degbey ◽

R. Oussou

Keyword(s):

West Africa ◽

Sequential Sampling ◽

Maize Weevil ◽

Sampling Plans ◽

Larger Grain Borer

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Distribution of and association between the larger grain borer Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) and the maize weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) in maize stores

Journal of Stored Products Research ◽

10.1016/j.jspr.2004.08.002 ◽

2005 ◽

Vol 41 (5) ◽

pp. 498-512 ◽

Cited By ~ 14

Author(s):

K.A. Vowotor ◽

W.G. Meikle ◽

J.N. Ayertey ◽

R.H. Markham

Keyword(s):

Sitophilus Zeamais ◽

Prostephanus Truncatus ◽

Maize Weevil ◽

Larger Grain Borer

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Resistance of three-way cross experimental maize hybrids to post-harvest insect pests, the larger grain borer ( Prostephanus truncatus) and maize weevil ( Sitophilus zeamais)

International Journal of Tropical Insect Science ◽

10.1017/s1742758411000075 ◽

2011 ◽

Vol 31 (1-2) ◽

pp. 3-12 ◽

Cited By ~ 15

Author(s):

Tadele Tefera ◽

Stephen Mugo ◽

Paddy Likhayo ◽

Yoseph Beyene

Keyword(s):

Insect Pests ◽

Sitophilus Zeamais ◽

Prostephanus Truncatus ◽

Maize Weevil ◽

Maize Hybrids ◽

Larger Grain Borer ◽

Post Harvest

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Efficacy of Chrysanthemum cinerariaefolium, Neorautanenia mitis and Gnidia kraussiana against Larger Grain Borer (Prostephanus truncatus Horn) and Maize Weevil (Sitophilus zeamays Motschulsky) on Maize (Zea mays L.) Grain Seeds

Journal of Entomology ◽

10.3923/je.2011.81.87 ◽

2010 ◽

Vol 8 (1) ◽

pp. 81-87 ◽

Cited By ~ 5

Author(s):

L.S. Mulungu ◽

B. Ndilahomba ◽

C.J. Nyange ◽

M.W. Mwatawala ◽

J.K. Mwalilino ◽

...

Keyword(s):

Zea Mays ◽

Zea Mays L ◽

Prostephanus Truncatus ◽

Maize Weevil ◽

Larger Grain Borer ◽

Chrysanthemum Cinerariaefolium

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The sequoia potato: A recently-introduced insect-resistant variety

American Potato Journal ◽

10.1007/bf02863510 ◽

1945 ◽

Vol 22 (4) ◽

pp. 97-103 ◽

Cited By ~ 8

Author(s):

M. E. Gardner ◽

Robert Schmidt ◽

F. J. Stevenson

Keyword(s):

Resistant Variety ◽

Insect Resistant

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Interspecific Competition and Grain-Hosts Selection of Maize Weevil, <i>Sitophilus zeamais</i> (Motsch.) (Coleoptera; Dryophtoridae) and Larger Grain Borer, <i>Prostephanus truncatus</i> (Horn) (Coleoptera; Bostrichidae)

Advances in Entomology ◽

10.4236/ae.2020.81003 ◽

2020 ◽

Vol 08 (01) ◽

pp. 34-45

Author(s):

Déthié Ngom ◽

Cheikh Thiaw ◽

Mbacké Sembène

Keyword(s):

Interspecific Competition ◽

Sitophilus Zeamais ◽

Prostephanus Truncatus ◽

Maize Weevil ◽

Larger Grain Borer ◽

Selection Of

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Relative resistance of maize varieties against maize weevil, Sitophilus zeamais (Motschulsky), (Coleoptera: Curcolionidae)

The Pakistan Journal of Agricultural Sciences ◽

10.21162/pakjas/21.45 ◽

2021 ◽

Vol 58 (04) ◽

pp. 1169-1176

Author(s):

Muhammad Mamoon-ur-Rashid

Keyword(s):

Weight Loss ◽

Adult Emergence ◽

Hardness Test ◽

Germination Percentage ◽

Sitophilus Zeamais ◽

Resistant Variety ◽

Grain Hardness ◽

Maize Weevil ◽

Percent Weight Loss ◽

Maize Varieties

The maize weevil, Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae), is one of the most damaging pests of stored cereals causing severe damage to stored grains. In current investigation, six open pollinated maize varieties viz. JALAL (white), AZAM (white), SADAF (yellow), ZARD LOCAL (yellow), KASHMIRI (yellow) and PAHARI (white) were screened out in the laboratory of Entomology Department, Faculty of Agriculture, Gomal University, Dera Ismail Khan, Pakistan for their relative susceptibility/resistance to maize weevil. The experiment was laid out following completely randomized design (CRD) with 5 replicates. Results revealed that minimum (23.8) days to adult emergence of maize weevil were recorded when it was reared on variety Azam; whereas; maximum (34.2) days were recorded on variety Sadaf. Total number of F1 adults emerged were maximum (91) in Azam while minimum (62) in variety Sadaf. Percent infestation of maize kernels was maximum (39.01%) in Azam while minimum (25.80%) was recorded in variety Sadaf. Percent weight loss was maximum in Azam i.e. 26.12% while minimum percent weight loss (16.88%) was recorded in Sadaf. Maximum longevity (68.4 days) of adult weevils was recorded on Azam while minimum (53.4 days) was recorded on variety Sadaf. All the tested varieties had no significant effect on the sex ratio of emerged weevils. High germination percentage was recorded in Sadaf (90%) whereas; low germination was found in Azam (57%). The grain hardness test indicated that yellow varieties including Sadaf, Zard local and Kashmiri had maximum hardness having 317, 302.33 and 296 N grain hardness, whereas; white varieties i.e. Pahari, Jalal and Azam had minimum values of grain hardness i.e. 264.33, 251.66 and 238.33 Newton. The present work has shown that Azam is the most susceptible variety and should not be stored for longer periods whereas; Sadaf is the resistant variety against maize weevil.

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