Larvicidal Efficacy of Crude Essential Oil (Leaf Extracts) of Pyrethrum (Chrysanthemum: Compositae), Eucalyptus camaldulensis Sm (Myrtaceae) and Nicotiana tabaccum (Tobacco L.) (Solanaceace) against Third Instar Larvae of the Malaria Vector Anopheles gambiae s.s. Giles (Diptera: Culicidae)

Abstract Background: The use of synthetic insecticides is responsible for many cases of resistance in insects. Therefore, the use of natural molecules of ecological interest with insecticidal properties turns out to be an alternative approach to the use of synthetic insecticides. This study aims at investigating the larvicidal, adulticidal activity and the chemical composition of the essential oil of Aeollanthus pubescens Benth on the major malaria vector Anopheles gambiae.The leaves of Aeollanthus pubescens were collected in the South of the Republic of Benin.Methods: Three reference strains of Anopheles gambiae s.s. such as Kisumu, Kiskdr and Acerkis were used. The standard WHO guideline for larvicides evaluation was used and the chemical composition of the essential oil was analysed by gas chromatography coupled to mass spectrometry. Adult mosquitoes were exposed to the fragment nets coated with the essential oil for 3 min. Probit regression analysis was used for LC50 , LC95 , KDT50 , and KDT95 calculations. The difference between the mortality‐dose regressions for the different strains was analysed using the likelihood ratio test (LRT). The Log-rank test was performed to evaluate the difference in survival between the strains.Results: Fourteen components were identified representing 98.31% of the total of oil. The major components were carvacrol (51.06 %), thymyle acetate (14.01 %) and ɣ-terpinene (10.60 %). The essential oil has remarkable larvicidal properties with LC50 of 29.57, 22.95, and 28.37 ppm respectively on Kisumu, Acerkis and Kiskdr strains. With the fragment net treated at 165 µg/cm2, the KDT50 of both Acerkis (1.71 s, p < 0.001) and Kiskdr (2.67 s, p < 0.001) individuals were significantly lower than that of Kisumu (3.77 s). The lifespan of the three mosquito strains decreased respectively to one day for Kisumu (p < 0.001), two days for Acerkis (p < 0.001) and three days for Kiskdr (p < 0.001) compared to their control.Conclusion: Our findings show that the Aeollanthus pubescens essential oil is an efficient larvicide and adulticide against malaria vector Anopheles gambiae. This bioinsecticidal activity is a promising discovery for the control of the resistant malaria-transmitting vectors.

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Evaluation of Ocimum suave essential oil against anthropophilic malaria vector Anopheles gambiae s.s after ten years of storage

Tanzania Journal of Health Research ◽

10.4314/thrb.v11i2.45211 ◽

2009 ◽

Vol 11 (2) ◽

Author(s):

EJ Kweka ◽

BJ Mwango'onde ◽

MJ Mahande ◽

FW Mosha ◽

EE Kimaro ◽

...

Keyword(s):

Essential Oil ◽

Anopheles Gambiae ◽

Malaria Vector ◽

Anopheles Gambiae S.S

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Chemical composition and insecticidal activity of Aeollanthus pubescens Benth leaf essential oil on the malaria vector Anopheles gambiae (Diptera: Culicidae)

10.1101/2020.11.25.397588 ◽

2020 ◽

Author(s):

Roméo Barnabé Bohounton ◽

Luc Salako Djogbénou ◽

Oswald Yédjinnavênan Djihinto ◽

Oronce Sedjro-Ludolphe Dedome ◽

Pierre Marie Sovegnon ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Anopheles Gambiae ◽

Malaria Vector ◽

Larval Mortality ◽

Alternative Approach ◽

Leaf Essential Oil ◽

Republic Of Benin ◽

The Republic ◽

Major Malaria Vector

AbstractThe use of synthetic insecticides is responsible for many cases of resistance in insects. Therefore, the use of natural molecules of ecological interest with insecticidal properties turns out to be an alternative approach to the use of synthetic insecticides. This study aims at investigating the larvicidal, adulticidal activity and the composition of the essential oil of Aeollanthus pubescens Benth on the major malaria vector Anopheles gambiae.The leaves of Aeollanthus pubescens were collected in the South of the Republic of Benin. Three reference strains of Anopheles gambiae s.s. such as Kisumu, Kiskdr and Acerkis were used. The chemical composition of the essential oil was analysed by gas chromatography coupled to mass spectrometry. Larvae were exposed to the essential oil extract for 24 h. Adult mosquitoes were exposed to the fragment nets coated with the essential oil for 3 min. Larval mortality and adult survivorship were monitored.Fourteen components were identified representing 98.31% of the total of oil. The major components were carvacrol (51.06 %), thymyle acetate (14.01 %) and γ-terpinene (10.60 %). The essential oil has remarkable larvicidal properties with LC50 of 29.26, 22.65, and 28.37 ppm respectively on Kisumu, Acerkis and Kiskdr strains. With the fragment net treated at 165 µg/cm2, the KDT50 of both Acerkis (1.71 s, p < 0.001) and Kiskdr (2.67 s, p < 0.001) individuals were significantly lower than that of Kisumu (3.77 s). The lifespan of the three mosquito strains decreased respectively to one day for Kisumu (p < 0.001), two days for Acerkis (p < 0.001) and three days for Kiskdr (p < 0.001) compared to their control.Our findings show that the Aeollanthus pubescens essential oil is an efficient larvicide and adulticide against malaria vector Anopheles gambiae. This bioinsecticidal activity is a promising discovery for the control of the resistant malaria-transmitting vectors.

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Adulticidal Activity of Hyptis suaveolens, Chenopodium ambrosioides and Lippia adoensis Leaf Extracts and Essential Oils against Anopheles gambiae (Diptera: Culicidae)

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2021/v40i231249 ◽

2021 ◽

pp. 18-32

Author(s):

Kary Mallam Oumarou ◽

Lame Younoussa ◽

Jacob Dogbaga Langsi ◽

Pierre Saotoing ◽

Elias Nchiwan Nukenine

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Anopheles Gambiae ◽

Plant Extracts ◽

Chemical Constituents ◽

Fats And Oils ◽

Chenopodium Ambrosioides ◽

Leaf Extracts ◽

Hyptis Suaveolens ◽

Lippia Adoensis

The study was undertaken to evaluate the adulticidal efficacy of the methanolic leaf extracts and essential oils of Chenopodium ambrosioides, Hyptis suaveolens and Lippia adoensis against adults of Anopheles gambiae. A chemical profile of each plant extracts (qualitative phytochemical screening) and essential oil (GC/MS analysis) was also determined. Doses of 125, 250, 500 and 1000 mg/bottle of plant extracts and 25, 50, 100 and 200 mg/bottle of essential oils were prepared and tested using CDC bottles. In each plant extract, alkaloids, flavonoids, saponins, tannins, phenolic groups, terpenoids, fats and oils were presents. Major chemical constituents of essential oils were thymol (27.09%), sabinene (18.93%) and 4-carene (52.88%) in L. adoensis, H. suaveolens and C. ambrosioides, respectively. L. adoensis extract (at 1000 mg/bottle) and essential oil of H. suaveolens (at 200 mg/bottle) were the most potent causing 100% mortality of An. gambiae adults, 24 h post-exposure. Methanolic extract of L. adoensis (LC50= 20.20 mg/bottle) was the most effective compared to other extracts. Similarly, essential oil of H. suaveolens (LC50= 5.27 mg/bottle) was revealed as the most toxic on An. gambiae adults compared to other oils. Therefore, the extracts of L. adoensis and essential oil of H. suaveolens showed a high activity and should be recommended to be promoting as natural bioinsecticides to control mosquito adults.

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