scholarly journals Aeollanthus Pubescens Benth Leaf Essential Oil: Its Chemical Composition and the Insecticidal Activity Against the Malaria Vector Anopheles Gambiae (Diptera: Culicidae)

2021 ◽  
Author(s):  
Roméo Barnabé BOHOUNTON ◽  
Luc Djogbenou ◽  
Oswald Yédjinnavênan Djihinto ◽  
Oronce Sedjro-Ludolphe Dedome ◽  
Pierre Marie Sovegnon ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Anopheles Gambiae ◽  
Malaria Vector ◽  
Rank Test ◽  
Ratio Test ◽  
Probit Regression ◽  
Anopheles Gambiae S.S ◽  
The Difference ◽  
Different Strains

Abstract Background: The use of synthetic insecticides is responsible for many cases of resistance in insects. Therefore, the use of natural molecules of ecological interest with insecticidal properties turns out to be an alternative approach to the use of synthetic insecticides. This study aims at investigating the larvicidal, adulticidal activity and the chemical composition of the essential oil of Aeollanthus pubescens Benth on the major malaria vector Anopheles gambiae.The leaves of Aeollanthus pubescens were collected in the South of the Republic of Benin.Methods: Three reference strains of Anopheles gambiae s.s. such as Kisumu, Kiskdr and Acerkis were used. The standard WHO guideline for larvicides evaluation was used and the chemical composition of the essential oil was analysed by gas chromatography coupled to mass spectrometry. Adult mosquitoes were exposed to the fragment nets coated with the essential oil for 3 min. Probit regression analysis was used for LC50 , LC95 , KDT50 , and KDT95 calculations. The difference between the mortality‐dose regressions for the different strains was analysed using the likelihood ratio test (LRT). The Log-rank test was performed to evaluate the difference in survival between the strains.Results: Fourteen components were identified representing 98.31% of the total of oil. The major components were carvacrol (51.06 %), thymyle acetate (14.01 %) and ɣ-terpinene (10.60 %). The essential oil has remarkable larvicidal properties with LC50 of 29.57, 22.95, and 28.37 ppm respectively on Kisumu, Acerkis and Kiskdr strains. With the fragment net treated at 165 µg/cm2, the KDT50 of both Acerkis (1.71 s, p < 0.001) and Kiskdr (2.67 s, p < 0.001) individuals were significantly lower than that of Kisumu (3.77 s). The lifespan of the three mosquito strains decreased respectively to one day for Kisumu (p < 0.001), two days for Acerkis (p < 0.001) and three days for Kiskdr (p < 0.001) compared to their control.Conclusion: Our findings show that the Aeollanthus pubescens essential oil is an efficient larvicide and adulticide against malaria vector Anopheles gambiae. This bioinsecticidal activity is a promising discovery for the control of the resistant malaria-transmitting vectors.

scholarly journals Chemical composition and the insecticidal activity of Aeollanthus pubescens leaf essential oil against Anopheles gambiae sensu stricto

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Roméo Barnabé Bohounton ◽  
Luc Salako Djogbénou ◽  
Oswald Yédjinnavênan Djihinto ◽  
Oronce Sedjro-Ludolphe Dedome ◽  
Pierre Marie Sovegnon ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Anopheles Gambiae ◽  
Malaria Vector ◽  
Sensu Stricto ◽  
World Health ◽  
Potential Candidate ◽  
Test Population ◽  
The Difference ◽  
Who Cone Bioassay

Abstract Background The excessive use of synthetic insecticides is responsible for many cases of resistance in insects. Therefore, the use of natural molecules of ecological interest with insecticidal properties is an alternative approach to the use of synthetic insecticides. The aim of this study is to investigating the larvicidal and adulticidal activity and the chemical composition of the essential oil of Aeollanthus pubescens on the major malaria vector, Anopheles gambiae. Methods Three reference strains of Anopheles gambiae sensu stricto (Kisumu, Kiskdr and Acerkis) were used in this study. The leaves of A. pubescens were collected in southern Benin. The standard World Health Organisation (WHO) guidelines for larvicide evaluation were used, and the chemical composition of the essential oil was analysed by gas chromatography coupled to mass spectrometry. Adult mosquitoes of each strain were exposed to pieces of net coated with the essential oil for 3 min using the WHO cone bioassay method. Probit regression analysis was used to determine the concentrations that would kill 50 and 95% of each test population (LC50, LC95) and the knockdown time for 50 and 95% of each test population (KDT50, and KDT95). The difference between the mortality–dose regressions for the different strains was analysed using the likelihood ratio test (LRT). The log-rank test was performed to evaluate the difference in survival between the strains. Results A total of 14 components were identified, accounting for 98.3% of total oil content. The major components were carvacrol (51.1%), thymyle acetate (14.0%) and ɣ-terpinene (10.6%). The essential oil showed larvicidal properties on the Kisumu, Acerkis and Kiskdr strains, with LC50 of 29.6, 22.9 and 28.4 ppm, respectively. With pieces of netting treated at 165 µg/cm2, the KDT50 of both Acerkis (1.71 s; Z = 3.34, P < 0.001) and Kiskdr (2.67 s; Z = 3.49, P < 0.001) individuals were significantly lower than that of Kisumu (3.8 s). The lifespan of the three mosquito strains decreased to 1 day for Kisumu (χ2 = 99, df = 1, P < 0.001), 2 days for Acerkis (χ2 = 117, df = 1, P < 0.001) and 3 days for Kiskdr (χ2 = 96.9, df = 1, P < 0.001). Conclusion Our findings show that A. pubescens essential oil has larvicide and adulticide properties against the malaria vector An. gambiae sensu stricto, suggesting that this essential oil may be a potential candidate for the control of the resistant malaria-transmitting vectors. Graphical Abstract

scholarly journals Chemical composition and insecticidal activity of Aeollanthus pubescens Benth leaf essential oil on the malaria vector Anopheles gambiae (Diptera: Culicidae)

2020 ◽  
Author(s):  
Roméo Barnabé Bohounton ◽  
Luc Salako Djogbénou ◽  
Oswald Yédjinnavênan Djihinto ◽  
Oronce Sedjro-Ludolphe Dedome ◽  
Pierre Marie Sovegnon ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Anopheles Gambiae ◽  
Malaria Vector ◽  
Larval Mortality ◽  
Alternative Approach ◽  
Leaf Essential Oil ◽  
Republic Of Benin ◽  
The Republic ◽  
Major Malaria Vector

AbstractThe use of synthetic insecticides is responsible for many cases of resistance in insects. Therefore, the use of natural molecules of ecological interest with insecticidal properties turns out to be an alternative approach to the use of synthetic insecticides. This study aims at investigating the larvicidal, adulticidal activity and the composition of the essential oil of Aeollanthus pubescens Benth on the major malaria vector Anopheles gambiae.The leaves of Aeollanthus pubescens were collected in the South of the Republic of Benin. Three reference strains of Anopheles gambiae s.s. such as Kisumu, Kiskdr and Acerkis were used. The chemical composition of the essential oil was analysed by gas chromatography coupled to mass spectrometry. Larvae were exposed to the essential oil extract for 24 h. Adult mosquitoes were exposed to the fragment nets coated with the essential oil for 3 min. Larval mortality and adult survivorship were monitored.Fourteen components were identified representing 98.31% of the total of oil. The major components were carvacrol (51.06 %), thymyle acetate (14.01 %) and γ-terpinene (10.60 %). The essential oil has remarkable larvicidal properties with LC50 of 29.26, 22.65, and 28.37 ppm respectively on Kisumu, Acerkis and Kiskdr strains. With the fragment net treated at 165 µg/cm2, the KDT50 of both Acerkis (1.71 s, p < 0.001) and Kiskdr (2.67 s, p < 0.001) individuals were significantly lower than that of Kisumu (3.77 s). The lifespan of the three mosquito strains decreased respectively to one day for Kisumu (p < 0.001), two days for Acerkis (p < 0.001) and three days for Kiskdr (p < 0.001) compared to their control.Our findings show that the Aeollanthus pubescens essential oil is an efficient larvicide and adulticide against malaria vector Anopheles gambiae. This bioinsecticidal activity is a promising discovery for the control of the resistant malaria-transmitting vectors.

scholarly journals Chemical Composition and Antimicrobial Activity of Essential Oil from Phytolacca dodecandra Collected in Ethiopia

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 342 ◽  
Author(s):  
Wondwosen Matebie ◽  
Wanchang Zhang ◽  
Guangbo Xie
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Solid Phase ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Microdilution Method ◽  
Phytolacca Dodecandra ◽  
The Difference

The essential oil from Phytolacca dodecandra, a traditional herb of Ethiopia, has been studied, including the chemical composition and antimicrobial activity. The difference between four P. dodecandra samples (P-1–P-4), which differed in gender or location, has also been analyzed. The essential oils were obtained by steam distillation, while the aromas were extracted by head space solid-phase microextraction (HS-SPME) and both were analyzed by gas chromatography- mass spectrometry (GC-MS). The oils’ antimicrobial activities were evaluated by the microdilution method against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans. Ninety one components, representing 88.37 to 94.01% of the aromas, were identified. The compositions of the aromas of four samples are mainly dominated by aldehydes and ketones: 2-nonanone (1.80–30.80%), benzaldehyde (4.99–25.99%), and sulcatone (2.34–5.87%). Sixty components representing 64.61 to 69.64% of the oils were identified, and phytone (3.04–21.23%), phytol (4.11–26.29%) and palmitic acid (1.49–23.87%) are the major compounds. No obvious antimicrobial activity was observed for all the four essential oils.

scholarly journals Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae s.s

2014 ◽  
Vol 7 (1) ◽  
pp. 209 ◽  
Author(s):  
France P Mdoe ◽  
Sen-Sung Cheng ◽  
Shandala Msangi ◽  
Gamba Nkwengulila ◽  
Shang-Tzen Chang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Anopheles Gambiae ◽  
Anopheles Gambiae S.S ◽  
Leaf Essential Oil
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