scholarly journals Comprehensive study of selection-value lines of zucchini on the level of damage by the yellow mosaic virus (ZYMV) and manifestation of other diseases

2021 ◽  
pp. 8-16
Author(s):  
Serhii Kondratenko ◽  
Oksana Sergienko ◽  
Yuliya Lancaster
Keyword(s):  
Powdery Mildew ◽  
Mosaic Virus ◽  
Correlation Coefficient ◽  
Abiotic Factors ◽  
Pearson Correlation ◽  
Zucchini Yellow Mosaic Virus ◽  
The United States ◽  
Yellow Mosaic Virus ◽  
Experimental Fact ◽  
Pairwise Correlation

The work is aimed at the selection of linear samples of zucchini (Cucurbita pepo L. var. Giromontia Duch.) with increased complex resistance to abiotic factors of cultivation – zucchini yellow mosaic virus (ZYMV), powdery mildew, bacteriosis and other viral pathogens. Selection studies on choosing virus-resistant zucchini lines from the United Kingdom, Italy, the United States and Spain have identified a number of important correlations levels of resistance to ZYMV and to harmful microorganisms and viruses, belonging to other genera. The assessment of the resistance of the selected zucchini lines to the complex of studied diseases was performed on a 9-point scale of the classifier of the Council for Mutual Economic Assistance (CMEA). In total, during 2017–2019, 20 lines of zucchini were studied in the field. According to the complex resistance to all pathogens (zucchini yellow mosaic virus, green speckled mosaic, white mosaic and cucumber mosaic, bacteriosis and fusarium wilt), four lines have been identified – LZ 17-11, RVL-19, LZ 17-45 and LZ 17-49, which were resistant at the level of points 7 and 9 on the scale of CMEA. The possibility of selecting potentially resistant linear zucchini genotypes for ZYMV based on correlations with the manifestation of other diseases that occurred in the field has been confirmed. The highest level of correlation 0.59<rp<1.0) is observed when comparing the degree of powdery mildew development and symptoms of ZYMV (17 statistically confirmed values of the pairwise correlation coefficient or 80.95 %). A similar indicator for the pair “bacteriosis/ZYMV” is 14 statistically confirmed values of the pairwise correlation coefficient or 66.67 % (–1.0<rp<0.99), and for the pair “field viruses of other origin/ZYMV” – 11 values, which is 52.38 % (–1.0<rp<0.93). Zucchini samples were identified, in which statistically significant correlations were observed for all pairs of comparative traits. Among them, the variety-standard Chaklun and 4 more lines – LZ 17-1, LZ 17-8, LZ 17-50 and LZ 17-44 (–1.0<rp<0.92). The analysis of correlations shows a complex genetic organization of signs of resistance in zucchini lines to the studied pathogens and a high dependence of the manifestation of these signs on the response of the genotype of the line. One of the proofs of this experimental fact is that in all comparative pairs of indicators of the degree of development of certain diseases there are linear genotypes with both positive and negative values of Pearson correlation coefficients (rp). The high level of statistically significant correlations revealed gives grounds for wide use of correlation analysis in selection work for choosing lines potentially resistant to the complex of the most common diseases in vegetable agrocenoses

scholarly journals Development and Field Evaluation of Multiple Virus-Resistant Bottle Gourd (Lagenaria siceraria)

Plant Disease ◽  
2013 ◽  
Vol 97 (8) ◽  
pp. 1057-1062 ◽  
Author(s):  
K.-S. Ling ◽  
A. Levi ◽  
S. Adkins ◽  
C. S. Kousik ◽  
G. Miller ◽  
...  
Keyword(s):  
South Carolina ◽  
Mosaic Virus ◽  
Broad Spectrum ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
The United States ◽  
Bottle Gourd ◽  
Yellow Mosaic Virus ◽  
Lagenaria Siceraria ◽  
Breeding Lines

In an effort to develop bottle gourd (Lagenaria siceraria) as a widely adapted rootstock for watermelon grafting, we sought to identify lines with broad resistance to several cucurbit viruses that are economically important in the United States. Preliminary analysis under greenhouse conditions indicated that the currently available commercial watermelon rootstocks were either highly susceptible or somewhat tolerant to one or more viruses. However, in greenhouse screening, several breeding lines of bottle gourd displayed broad-spectrum resistance to four viruses tested, including Zucchini yellow mosaic virus, Watermelon mosaic virus (WMV), Papaya ringspot virus watermelon strain (PRSV-W), and Squash vein yellowing virus. Resistance to PRSV-W and WMV was confirmed through field trials in two consecutive years at two different locations in South Carolina. Two breeding lines (USVL#1-8 and USVL#5-5) with broad-spectrum virus resistance could be useful materials for watermelon rootstock development.

scholarly journals GENETIC RELATIONSHIPS AMONG RESISTANCE TO ZUCCHINI YELLOW MOSAIC VIRUS, WATERMELON MOSAIC VIRUS, PAPAYA RINGSPOT VIRUS, AND POWDERY MILDEW IN MELON (CUCUMIS MELO)

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 913G-914
Author(s):  
Konstantinos Anagnostou ◽  
Molly Kyle ◽  
Rafael Perl-Treves
Keyword(s):  
Powdery Mildew ◽  
Mosaic Virus ◽  
Cucumis Melo ◽  
Genetic Relationships ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Relationship Of

We have studied the relationship of resistance to watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), papaya ringspot virus (PRSV), and powdery mildew (PM) in melon (Cucumis melo). We have confirmed monogenic dominant inheritance of these four resistances and report that PI414723-4S3, which was initially selected as a source of ZYMR, is also a source of dominant monogenic resistance to PRSV. Further, we observed departure from independent assortment for resistance to WMV and ZYMV in a study of 73 (UC Top Mark × PI414723-4S3) F3 families (χ2 = 39.87 significant at both 0.01 and 0.05 levels), indicating linkage between Wmv and Zym. The map distance between these resistance genes calculated from the number of recombinant families (RF% = 9.58) was 10.5 cM. Compari-sons among WMV, PM, ZYMV-PM, PRSV-PM, ZYMV-PRSV, and WMV-PRSV of 48 (TM × PI414723-4S3) F3 families, which were screened with all four pathogens, showed no consistent cosegregation.

scholarly journals Complete Genome Sequence of a Zucchini Yellow Mosaic Virus Isolated from Pumpkin in Oklahoma

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Vivek Khanal ◽  
Akhtar Ali
Keyword(s):  
United States ◽  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Zucchini Yellow Mosaic Virus ◽  
Growing Season ◽  
The United States ◽  
Yellow Mosaic Virus ◽  
Content Type

Zucchini yellow mosaic virus (ZYMV) (genus Potyvirus, family Potyviridae) was first isolated in Europe during the 1970s and in the United States in 1981. Here, we report the complete genome sequence of ZYMV isolate BL-67, isolated from pumpkin in Oklahoma during the 2016 growing season.

scholarly journals First Report of Zucchini yellow mosaic virus Associated with Leaf Crinkle and Yellow Mosaic Diseases of Cucurbit Plants in Mali

Plant Disease ◽  
2010 ◽  
Vol 94 (7) ◽  
pp. 923-923 ◽  
Author(s):  
W. S. Tsai ◽  
I. K. Abdourhamane ◽  
D. Knierim ◽  
J. T. Wang ◽  
L. Kenyon
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
The United States ◽  
Yellow Mosaic Virus ◽  
Nucleotide Identity ◽  
Watermelon Mosaic Virus ◽  
Virus Species ◽  
First Report

The aphid-transmitted Zucchini yellow mosaic virus (ZYMV; genus Potyvirus, family Potyviridae) has been reported to cause severe epidemics and yield losses in cucurbit crops worldwide (1). In Africa, ZYMV has been detected in Algeria, Egypt, Madagascar, Mauritius, Mayotte, Morocco, Nigeria, Reunion, South Africa, Sudan, Swaziland, and Tunisia (1). In April 2009, leaf yellowing, mosaic, crinkling, and curling were common on cucurbit plants in fields in Mali. Symptomatic leaf samples were collected from five cucumber (Cucumis sativus) plants in Kati, two watermelon (Citrullus lanatus) plants in Samanko, and one weedy melon (Cucumis sp.) plant in Baguineda. All samples tested positive for ZYMV and were negative for Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV), Papaya ringspot virus type W (PRSV-W), Watermelon mosaic virus (WMV), and Watermelon silver mottle virus (WSMoV) by double-antibody sandwich (DAS)-ELISA. They also tested negative for Melon yellow spot virus (MYSV) by indirect ELISA. Antibodies against ZYMV and WMV were obtained from DSMZ, Braunschweig, Germany, and those against CGMMV, MYSV, PRSV-W, and WSMoV were provided by Shyi-Dong Yeh, National Chung Hsing University, Taichung, Taiwan. Six ZYMV ELISA-positive samples (three cucumber, two watermelon, and the weedy melon sample) were also tested by reverse transcription (RT)-PCR using the potyvirus universal primer pair Sprimer1/Oligo(dT) (2). The expected 1.6-kb viral cDNA was amplified from all six samples and each was sequenced. All sequences obtained from cucumber (GenBank Accession Nos. HM005307, HM005308, and HM005309), watermelon (GenBank Accession Nos. HM005311 and HM005312), and weedy melon (GenBank Accession No. HM005310) isolates were 1,684 nucleotides (nt) long excluding the 3′ poly-A tails. They comprised the 3′-terminal of the NIb region (1 to 633 nt), the coat protein region (634 to 1473 nt), and the 3′-untranslated region (1,474 to 1,684 nt). Because the sequences shared high nucleotide identity (98.3 to 99.7%), these isolates were considered to be the same virus species. When the sequences were compared by BLASTn searching in GenBank and analyzed by DNAMAN Sequence Analysis Software (Lynnon Corporation, St-Louis, Pointe-Claire, Quebec, Canada), they were found to have the greatest nucleotide identity (97.4 to 98.0%) with the Connecticut strain of ZYMV (ZYMV-Connecticut; GenBank Accession No. D00692), within a clade of isolates from China, Italy, Japan, and the United States. When assessed separately, their coat protein (97.7 to 98.3% nucleotide and 98.9 to 99.6% amino acid identity) and 3′-untranslated regions (96.7 to 97.2% identity) also had greatest homology with ZYMV-Connecticut. To our knowledge, this is the first report of ZYMV infecting cucurbit plants in Mali. ZYMV should be taken into consideration when breeding cucurbit crops for this region, and managing viral diseases. References: (1) C. Desbiez et al. Plant Pathol. 46:809, 1997. (2) W. S. Tsai et al. Plant Dis. 94:378, 2010.

scholarly journals First Report of Bidens mottle virus Causing Mosaic and Leaf Deformation in Garland Chrysanthemum and Lettuce in Taiwan

Plant Disease ◽  
2012 ◽  
Vol 96 (3) ◽  
pp. 464-464 ◽  
Author(s):  
Y. K. Chen ◽  
J. Y. Lee
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Chenopodium Quinoa ◽  
The United States ◽  
Mottle Virus ◽  
Yellow Mosaic Virus ◽  
Degenerate Primers ◽  
First Report ◽  
Cdna Sequences ◽  
Original Host

Garland chrysanthemum (Chrysanthemum coronarium) and lettuce (Lactuca sativa) are winter vegetables in Taiwan. Turnip mosaic virus (TuMV) and Lettuce mosaic virus (LMV) were potyviruses commonly isolated from garland chrysanthemum and lettuce, respectively (1). Symptoms of mosaic and deformation in leaves and stunting of plants have been observed in both Compositae crops in the fields since 2007 in the Chiayi area and with an increasing incidence in recent years (26 and 33% in garland chrysanthemum and lettuce, respectively). Filamentous virus particles (approximately 780 × 13 nm) in the crude sap and pinwheel inclusions in infected cells can be observed in the preparations of both diseased hosts with electron microscopy. However, TuMV, LMV, and other potyviruses (Bean yellow mosaic virus, Papaya ringspot virus, and Zucchini yellow mosaic virus) were not detectable in diseased samples by ELISA and western blotting tests, indicating a new potyvirus infection. Virus cultures were isolated from infected garland chrysanthemum and lettuce separately via mechanical inoculations in Chenopodium quinoa. Each isolate was mechanically inoculated to their original host individually and all caused symptoms similar to that observed in the field, indicating their pathogenicity to their original host. A cDNA fragment consisting of partial nuclear inclusion (NIb) and coat protein (CP) genes were amplified with potyvirus degenerate primers (forward: 5′-GGBAAYAATAGTGGNCAACC and reverse: 5′-GGGGAGGTGCCGTTCTCDATRCACCA) and was found to share 90% nucleotide sequence identity to that of Bidens mottle virus (BiMoV; GenBank Accession No. AF538686). The sequences of the CP gene and 3′ untranslated region (3′-UTR) of tested viruses were further amplified with a specific primer of BiMoV CP and oligo-dT in reverse transcription-PCR. The amplified fragments were cloned, sequenced, and the combined cDNA sequences were deposited in GenBank (Accession No. AB491763 for isolate garland chrysanthemum and Accession No. AB491764 for isolate lettuce). Sequence analysis showed that both cloned sequences shared more than 97% nucleotide similarity to that of BiMoV. The amino acid sequence of the CP of both isolates shared a 99.3% identity and a 98.9 to 99.3% identity to that of other BiMoV isolates deposited in GenBank. BiMoV was first described to be infecting lettuce and Cichorium endivia in the United States (4) and was first reported in sunflower and calendula in Taiwan recently (2,3). To our knowledge, this is the first report of the occurrence of BiMoV in garland chrysanthemum and lettuce in Taiwan. References: (1) Y. K. Chen et al. Plant Pathol. Bull. 5:55,1996. (2) C.-H. Huang and F.-J. Jan. Plant Dis. 95:362, 2011. (3) J. Y. Liao et al. Arch. Virol. 154:723, 2009. (4) F. Youssef et al. Arch. Virol 153:227, 2008.

scholarly journals Inheritance of Resistance to the Florida Strain of Zucchini Yellow Mosaic Virus in Watermelon

HortScience ◽  
1991 ◽  
Vol 26 (4) ◽  
pp. 407-408 ◽  
Author(s):  
R. Provvidenti
Keyword(s):  
Mosaic Virus ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Recessive Gene ◽  
The United States ◽  
Yellow Mosaic Virus ◽  
Plant Selection ◽  
Backcross Populations ◽  
Single Plant Selection ◽  
High Level

A high level of resistance to zucchini yellow mosaic virus (ZYMV) was found in four landraces of Citrullus lanatus (Thunb.) MatSum. & Nakai (PI 482322, PI 482299, PI 482261, and PI 482308) originally collected in Zimbabwe. This resistance is specific to the Florida strain of the virus (ZYMV-FL), which prevails in the United States. Inheritance studies were conducted by using a single-plant selection (PP261-I) of PI 48226I and the ZYMV-susceptible watermelon cultivar New Hampshire Midget. In F1, F2, and reciprocal backcross populations, resistance was conferred by a single recessive gene to which the symbol zym is assigned. There was no linkage between zym and one of the three homodimer bands of the isozyme phosphoglucoisomerase (Pgi-2b), which was found in PP261-1, and in other ZYMV-FL resistant plants.

scholarly journals Identification and Utility of Markers Linked to the Zucchini Yellow Mosaic Virus Resistance Gene in Watermelon

2009 ◽  
Vol 134 (5) ◽  
pp. 529-534 ◽  
Author(s):  
Karen R. Harris ◽  
Kai-Shu Ling ◽  
W. Patrick Wechter ◽  
Amnon Levi
Keyword(s):  
United States ◽  
Genetic Distance ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Zucchini Yellow Mosaic Virus ◽  
Recessive Gene ◽  
The United States ◽  
Yellow Mosaic Virus ◽  
Recurrent Parent ◽  
Resistant Parent

Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses affecting watermelon [Citrullus lanatus (Thunb.) Matsun & Nakai var. lanatus] in the United States. The ZYMV-Florida strain (ZYMV-FL) is considered a major limitation to commercial watermelon production in the United States. Inheritance of resistance to ZYMV-FL is conferred by a recessive gene. This report describes the identification of single-reaction, polymerase chain reaction-based markers linked to the ZYMV-FL resistance gene in watermelon. In this study, we identified a marker ZYMV-resistant polymorphism (ZYRP) linked to the ZYMV-FL resistance gene locus (genetic distance of 8 cM) in an F2 population, and in a backcross one to the resistant parent population (BC1R) (genetic distance of 13 cM). The identification of a single nucleotide polymorphism within the ZYRP marker for the parental genotypes allowed the development of a sequence-characterized amplification region marker linked to the ZYMV-FL resistance gene. Experiments using a BC2F2 population derived from the U.S. Plant Introduction 595203 (C. lanatus var. lanatus) and the recurrent parent ‘Charleston Gray’ indicated that the ZYRP marker can be used in marker-assisted selection to identify genotypes containing the gene conferring ZYMV-FL resistance in watermelon.

scholarly journals `Hannah's Choice F1': A New Muskmelon Hybrid with Resistance to Powdery Mildew, Fusarium Race 2, and Potyviruses

HortScience ◽  
2005 ◽  
Vol 40 (2) ◽  
pp. 492-493 ◽  
Author(s):  
Mark J. Henning ◽  
Henry M. Munger ◽  
Molly M. Jahn
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Mosaic Virus ◽  
Pseudomonas Syringae ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Didymella Bryoniae ◽  
Multiple Disease Resistance ◽  
Race 2

`Hannah's Choice F1' is a new, high quality eastern type muskmelon (Cucumis melo L.) with multiple disease resistance. It was developed in the Department of Plant Breeding at the Cornell University Agricultural Experiment Station in Ithaca, N.Y. It is well adapted for northeastern U.S. conditions and shows potential for good adaptation in the northwest. With multiple disease resistance it is well suited for home gardeners, market gardeners, and commercial growers. `Hannah's Choice F1' has excellent resistance to powdery mildew races 1 and 2 (Podosphaera xanthi) and some tolerance to Fusarium root rot (Fusarium oxysporum f. sp. melonis) race 2. In addition, it has resistance to watermelon mosaic virus (WMV), papaya ringspot virus (PRSV), and zucchini yellow mosaic virus (ZYMV). This is the first commercial melon to have combined resistance to these three potyviruses. Also, it has shown some field tolerance to spider mites (Tetranychus urticae). Lastly, it has shown some field tolerance to downy mildew (Pseudoperonospora cubensis), angular leaf spot (Pseudomonas syringae pv. lachrymans), and gummy stem blight (Didymella bryoniae). In 2001, 2002, and 2003 it was grown in replicated trials in New York and in 2002 and 2003 in Oregon.

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