Essential Role of Singlet Oxygen Species in Cytochrome P450-dependent Substrate Oxygenation by Rat Liver Microsomes

2005 ◽  
Vol 20 (1) ◽  
pp. 14-23 ◽  
Author(s):  
Seiko Hayashi ◽  
Hiroyuki Yasui ◽  
Hiromu Sakurai
Keyword(s):  
Cytochrome P450 ◽  
Singlet Oxygen ◽  
Rat Liver ◽  
Essential Role ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Oxygen Species ◽  
Singlet Oxygen Species

Lipid Peroxidation and Cherniluminescence during Naproxen Metabolism in Rat Liver Microsomes

1994 ◽  
Vol 13 (12) ◽  
pp. 831-838 ◽  
Author(s):  
Hiroyuki Yokoyama ◽  
Toshiharu Horie ◽  
Shoji Awazu
Keyword(s):  
Lipid Peroxidation ◽  
Singlet Oxygen ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Thiobarbituric Acid ◽  
Rat Liver Microsomes ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Microsomal Suspension ◽  
Weight Protein

1 Rat liver microsomal suspension containing NADPH and MgCl2 was incubated at 37°C with naproxen, a non-steroidal anti-inflammatory drug. Thiobarbituric acid reactive substances (TBA-RS), high molecular weight protein aggregates and fluorescent substances were formed in the microsomal suspension. 2 Chemiluminescence was produced from the microsomal suspension. This chemiluminescence production was well correlated to the TBA-RS formation, indicating that the chemiluminescence production was closely associated with the lipid peroxidation. 3 The addition of SKF-525A to the microsomal suspension inhibited the production of TBA-RS, chemiluminescence and 6-demethylnaproxen (6-DMN), the oxidative product of naproxen. Further, the antioxidant, α-tocopherol and singlet oxygen quenchers like histidine, dimethylfuran and 1,4-diazabicyclo[2,2,2]octane strikingly inhibited the productions of chemiluminescence and TBA-RS. 4 Neither naproxen nor 6-DMN caused lipid peroxidation in the absence of NADPH. Thus, lipid peroxidation and chemiluminescence during the oxidation of naproxen in liver microsomes was suggested to be provoked by reactive oxygen species and an origin of chemiluminescence was shown to be singlet oxygen.

Oxidation of methyl- and ethyl- tertiary-butyl ethers in rat liver microsomes: role of the cytochrome P450 isoforms

1998 ◽  
Vol 72 (4) ◽  
pp. 207-214 ◽  
Author(s):  
Alessandra Turini ◽  
Giada Amato ◽  
Vincenzo Longo ◽  
Pier Giovanni Gervasi
Keyword(s):  
Cytochrome P450 ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Tertiary Butyl ◽  
Cytochrome P450 Isoforms

scholarly journals Involvement of cytochrome P450 2E1 in the (ω–1)-hydroxylation of oleic acid in human and rat liver microsomes

1998 ◽  
Vol 39 (6) ◽  
pp. 1210-1219 ◽  
Author(s):  
Fadi Adas ◽  
François Berthou ◽  
Daniel Picart ◽  
Patrick Lozac'h ◽  
Françoise Beaugé ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Oleic Acid ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Cytochrome P450 2E1
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