Discovery of Genetic Variants in CYP1D1: Implication for Functional Integrity of CYP1D1 in Cynomolgus Macaques and Rhesus Macaques

Intrauterine sperm injection for artificial insemination is difficult in cynomolgus macaques (Macaca fascicularis) and rhesus macaques (M. mulatta) due to the complex structure of the cervical canal, which differs from that of humans. Despite the availability of several artificial insemination methods for macaques, pregnancy rates are inconsistent, and details regarding ovulation are unclear, thus warranting more effective methods. Therefore, we developed an effective, ultrasound-guided, transabdominal intrauterine artificial insemination method for cynomolgus macaques that involves timing sperm injection to coincide with the periovulation phase estimated according to rapid hormone measurement. We performed our intrauterine artificial insemination on 6 female macaques; 4 of the 5 animals that were predicted to have ovulated soon after insemination became pregnant, whereas the 1 macaque that was predicted not to have ovulated did not. Furthermore, we saw no evidence of injury, such as a conspicuous needle hole or bleeding on the surface of or inside the uterus, nor did our method result in any abnormalities in the mothers or their offspring. Thus, our ultrasound-guided, transabdominal, intrauterine artificial insemination method is rapid, safe, and effective in cynomolgus macaques.

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Genetic Variants of Glutathione S-Transferase GSTT1 and GSTT2 in Cynomolgus Macaques: Identification of GSTT Substrates and Functionally Relevant Alleles

Chemical Research in Toxicology ◽

10.1021/acs.chemrestox.8b00198 ◽

2018 ◽

Vol 31 (10) ◽

pp. 1086-1091 ◽

Cited By ~ 3

Author(s):

Yasuhiro Uno ◽

Norie Murayama ◽

Masami Kato ◽

Saki Tanaka ◽

Tomoko Ohkoshi ◽

...

Keyword(s):

Genetic Variants ◽

Glutathione S Transferase ◽

Cynomolgus Macaques

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Functionally relevant genetic variants of glutathione S-transferase GSTM5 in cynomolgus and rhesus macaques

Xenobiotica ◽

10.1080/00498254.2018.1524187 ◽

2018 ◽

Vol 49 (8) ◽

pp. 995-1000 ◽

Cited By ~ 3

Author(s):

Yasuhiro Uno ◽

Norie Murayama ◽

Kazuaki Tamura ◽

Hiroshi Yamazaki

Keyword(s):

Genetic Variants ◽

Rhesus Macaques ◽

Glutathione S Transferase

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Variability in the number of infraorbital foramina in rhesus macaques ( Macaca mulatta ) and cynomolgus macaques ( Macaca fascicularis )

The Anatomical Record ◽

10.1002/ar.24478 ◽

2020 ◽

Author(s):

Justyna Marchewka ◽

Kamil Mrożek ◽

Bartosz Leszczyński ◽

Andrzej Wróbel ◽

Henryk Głąb

Keyword(s):

Macaca Mulatta ◽

Macaca Fascicularis ◽

Rhesus Macaques ◽

Cynomolgus Macaques

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Infection of cynomolgus macaques (Macaca fascicularis) and rhesus macaques (Macaca mulatta) with different wild-type measles viruses

Journal of General Virology ◽

10.1099/vir.0.82804-0 ◽

2007 ◽

Vol 88 (7) ◽

pp. 2028-2034 ◽

Cited By ~ 38

Author(s):

H. Sittana El Mubarak ◽

Selma Yüksel ◽

Geert van Amerongen ◽

Paul G. H. Mulder ◽

Maowia M. Mukhtar ◽

...

Keyword(s):

Measles Virus ◽

Mononuclear Cells ◽

Rhesus Macaques ◽

Wild Type ◽

Immunological Parameters ◽

Peripheral Blood Mononuclear ◽

Cynomolgus Monkeys ◽

Cynomolgus Macaques ◽

Measles Vaccination ◽

Kinetics Of

Both rhesus and cynomolgus macaques have been used as animal models for measles vaccination and immunopathogenesis studies. A number of studies have suggested that experimental measles virus (MV) infection induces more-characteristic clinical features in rhesus than in cynomolgus monkeys. In the present study, both macaque species were infected with two different wild-type MV strains and clinical, virological and immunological parameters were compared. The viruses used were a genotype C2 virus isolated in The Netherlands in 1991 (MV-Bil) and a genotype B3 virus isolated from a severe measles case in Sudan in 1997 (MV-Sudan). Following infection, all rhesus monkeys developed a skin rash and conjunctivitis, which were less obvious in cynomolgus monkeys. Fever was either mild or absent in both species. Virus reisolation profiles from peripheral blood mononuclear cells and broncho-alveolar lavage cells and the kinetics of MV-specific IgM and IgG responses were largely identical in the two animal species. However, in animals infected with MV-Sudan, viraemia appeared earlier and lasted longer than in animals infected with MV-Bil. This was also reflected by the earlier appearance of MV-specific serum IgM antibodies after infection with MV-Sudan. Collectively, these data show that cynomolgus and rhesus macaques are equally susceptible to wild-type MV infection, although infection in the skin seems to follow a different course in rhesus macaques. MV-Sudan proved more pathogenic for non-human primates than MV-Bil, which may render it more suitable for use in future pathogenesis studies.

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Genetic Variants of CYP3A4 and CYP3A5 in Cynomolgus and Rhesus Macaques

Drug Metabolism and Disposition ◽

10.1124/dmd.109.029710 ◽

2009 ◽

Vol 38 (2) ◽

pp. 209-214 ◽

Cited By ~ 48

Author(s):

Yasuhiro Uno ◽

Akinori Matsushita ◽

Naoki Osada ◽

Shotaro Uehara ◽

Sakae Kohara ◽

...

Keyword(s):

Genetic Variants ◽

Rhesus Macaques

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Markers of hepatitis E among the population of the Greater Sochi and in monkeys of the Adler primate center

Voprosy Virusologii ◽

10.18821/0507-4088-2016-61-4-176-180 ◽

2016 ◽

Vol 61 (4) ◽

pp. 176-180 ◽

Cited By ~ 1

Author(s):

L. I. Korzaia ◽

V. V. Keburia ◽

D. I. Dogadov ◽

B. A. Lapin ◽

K. K. Kyuregyan ◽

...

Keyword(s):

Macaca Mulatta ◽

Rhesus Macaques ◽

Hepatitis E ◽

Papio Hamadryas ◽

Cancer Center ◽

Papio Anubis ◽

Chlorocebus Aethiops ◽

Medical Institutions ◽

Cynomolgus Macaques ◽

Clinic Hospital

Serum from humans (n = 646) and monkeys (n = 1867) collected during the period 1999-2013 was tested by enzyme immunoassay. Anti-HEV IgG was detected significantly more frequently (P ≥ 0.001) in rhesus macaques (Macaca mulatta) - 45.1 ± 1.6% (n = 1001) than in cynomolgus macaques (M. fascicularis) 16.2 ± 1.8% (n = 426). Single seropositive individuals were found among M. nemestrina - 4.0±2.8% (n = 50). Anti-HEV was not detected in the sera of green monkeys (Chlorocebus aethiops) - n = 162, Papio hamadryas (n = 124), and Papio anubis - n = 104. The presence of the anti-HEV IgM indicating the cases of fresh infection in Macaca mulatta - 2.1 ± 0.5% (n = 717) and M. fascicularis - 3.5 ± 1.3% (n = 266) is of great significance. The overall frequency of detection of the anti-HEV IgG among the staff of the Adler Primate Center - 6.8 ± 2.3% (n = 118) was significantly lower (P ≤ 0.001) than among the population of the Greater Sochi - 15.9% ± 1.6 (n = 528). It is important that only in patients of medical institutions (clinic, hospital, cancer center), anti-HEV IgM were detected (2.7-11.8%) along with anti-HEV IgG (15-23.5%), thereby indicating the presence of acute cases of HEV infection among this population. HEV RNA was not detected in the serum of anti-HEV IgM-positive people and monkeys. Seroepidemiological data do not confirm the assumption on the ability of seropositive monkeys of Macaca genus to be a natural reservoir of HEV infection for humans.

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In vitro Characterization of Fitness and Convalescent Antibody Neutralization of SARS-CoV-2 Cluster 5 Variant Emerging in Mink at Danish Farms

Frontiers in Microbiology ◽

10.3389/fmicb.2021.698944 ◽

2021 ◽

Vol 12 ◽

Author(s):

Ria Lassaunière ◽

Jannik Fonager ◽

Morten Rasmussen ◽

Anders Frische ◽

Charlotta Polacek ◽

...

Keyword(s):

Amino Acid ◽

Reference Strain ◽

Rhesus Macaques ◽

Amino Acid Residues ◽

Susceptible Animal ◽

In Vitro Characterization ◽

Cynomolgus Macaques ◽

Animal Hosts

In addition to humans, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can transmit to animals that include hamsters, cats, dogs, mink, ferrets, tigers, lions, cynomolgus macaques, rhesus macaques, and treeshrew. Among these, mink are particularly susceptible. Indeed, 10 countries in Europe and North America reported SARS-CoV-2 infection among mink on fur farms. In Denmark, SARS-CoV-2 spread rapidly among mink farms and spilled-over back into humans, acquiring mutations/deletions with unknown consequences for virulence and antigenicity. Here we describe a mink-associated SARS-CoV-2 variant (Cluster 5) characterized by 11 amino acid substitutions and four amino acid deletions relative to Wuhan-Hu-1. Temporal virus titration, together with genomic and subgenomic viral RNA quantitation, demonstrated a modest in vitro fitness attenuation of the Cluster 5 virus in the Vero-E6 cell line. Potential alterations in antigenicity conferred by amino acid changes in the spike protein that include three substitutions (Y453F, I692V, and M1229I) and a loss of two amino acid residues 69 and 70 (ΔH69/V70), were evaluated in a virus microneutralization assay. Compared to a reference strain, the Cluster 5 variant showed reduced neutralization in a proportion of convalescent human COVID-19 samples. The findings underscore the need for active surveillance SARS-CoV-2 infection and virus evolution in susceptible animal hosts.

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