Representative compounds from three classes of herbicides (atrazine, linuron, and treflan) were studied to determine ambient levels in the Chesapeake Bay and its tributaries during portions of 1980. All levels were 1 μg/L or less, and all three herbicides exhibited non-conservative behavior in the estuary. Concentrations of herbicides in runoff from a defined watershed did not exceed 9 μg/L. Degradation rates for all three herbicides in estuarine sediment-water systems were 2–10 times greater than those reported for soils. Equilibrium sorption constants (Koc) of estuarine sediments were similar to soils, but suspended colloids were found to sorb atrazine and linuron 10–30 times more strongly on a per gram of carbon basis, suggesting that refractory hydrophobic pollutants may be transported greater distances in the water column than previously assumed. However, the large degradative capacity of the estuarine community may act to prevent transport of labile organics from the land to the oceans.