Selection for Monogenic Pest Resistance Traits with Coupling‐ and Repulsion‐Phase RAPD Markers

Crop Science ◽  
1994 ◽  
Vol 34 (4) ◽  
pp. 1061-1066 ◽  
Author(s):  
Scott D. Haley ◽  
Lucia Afanador ◽  
James D. Kelly
Keyword(s):  
Rapd Markers ◽  
Pest Resistance ◽  
Repulsion Phase ◽  
Selection For ◽  
Resistance Traits

scholarly journals Marker-Assisted Selection for Resistance to Black Shank Disease in Tobacco

Plant Disease ◽  
2002 ◽  
Vol 86 (12) ◽  
pp. 1303-1309 ◽  
Author(s):  
E. S. Johnson ◽  
M. F. Wolff ◽  
E. A. Wernsman ◽  
R. C. Rufty
Keyword(s):  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Rapd Marker ◽  
Black Shank ◽  
Ploidy Levels ◽  
Complete Linkage ◽  
Repulsion Phase ◽  
Selection For ◽  
Haploid Plants

Bulked segregant (BSA) and random amplified polymorphic DNA (RAPD) analyses were used to identify markers linked to the dominant black shank resistance gene, Ph, from flue-cured tobacco (Nicotiana tabacum) cv. Coker 371-Gold. Sixty RAPD markers, 54 in coupling and 6 in repulsion phase linkage to Ph, were identified in a K 326-derived BC1F1 (K 326-BC1F1) doubled haploid (DH) population. Thirty RAPD markers, 26 in coupling and 4 in repulsion phase linkage to Ph, were used to screen 149 K 326-BC2F1 haploid plants. Complete linkage between the 26 coupling phase markers and Ph was confirmed by screening 149 K 326-BC2F1 DH lines produced from the haploid plants in black shank nurseries. RAPD markers OPZ-5770 in coupling and OPZ-7370 in repulsion phase linkage were used to select plants homozygous for the Ph gene for further backcrossing to the widely grown flue-cured cultivar K 326. Black shank disease nursery evaluation of 11 K 326-BC4S1 lines and their testcross hybrids to a susceptible tester confirmed linkage between Ph and OPZ-5770. The results demonstrated the efficiency of marker-assisted selection for Ph using a RAPD marker linked in coupling and repulsion. Complete linkage between 26 RAPD markers and the Ph gene was confirmed in the K 326-BC5 generation, and RAPD phenotypes were stable across generations and ploidy levels. These RAPD markers are useful in marker-assisted selection for Ph, an important black shank resistance gene in tobacco.

Directed Mass Selection for Developing Multiple Pest Resistance and Conserving Germplasm in Alfalfa

1972 ◽  
Vol 1 (1) ◽  
pp. 106-111 ◽  
Author(s):  
C. H. Hanson ◽  
T. H. Busbice ◽  
R. R. Hill ◽  
O. J. Hunt ◽  
A. J. Oakes
Keyword(s):  
Pest Resistance ◽  
Mass Selection ◽  
Selection For

Identification of RAPD markers linked to a gene governing cadmium uptake in durum wheat

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 543-547 ◽  
Author(s):  
Greg A. Penner ◽  
Leslie J. Bezte ◽  
Dave Leisle ◽  
John Clarke
Keyword(s):  
Durum Wheat ◽  
Gel Electrophoresis ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Cadmium Uptake ◽  
Cadmium Content ◽  
Diverse Range ◽  
Selection For

Temperature sweep gel electrophoresis in combination with random amplified polymorphic DNA analysis was employed to detect two markers for a single gene governing low cadmium uptake in western Canadian durum wheat (Triticum turgidum L. var. durum). Analysis of progeny derived from a cross of the high cadmium accumulating cultivar Kyle by the low cadmium accumulating cultivar Nile resulted in linkage estimates of 4.6 (OPC-20) and 21.2 (UBC-180) cM. The closest marker (OPC-20) was shown to be useful for making low cadmium uptake selections from two other sources of low cadmium, 'Biodur' and 'Hercules'. The marker was further used to determine the genetic basis of resistance in 20 introduced durum wheat lines. Within this diverse range of germplasm the marker was correlated with cadmium contents as expected in all but two cases. Plant breeding selection for low cadmium genotypes is hindered by the high cost of chemical determination of cadmium content. Marker assisted selection for a low cadmium uptake gene offers an effective alternative.Key words: cadmium, durum wheat, RAPD markers, marker assisted selection, temperature sweep gel electrophoresis.

Identification of coupling and repulsion phase RAPD markers for powdery mildew resistance gene er-1 in pea

Genome ◽  
1998 ◽  
Vol 41 (3) ◽  
pp. 440-444 ◽  
Author(s):  
K R Tiwari ◽  
G A Penner ◽  
T D Warkentin
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Rapd Markers ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Powdery Mildew Resistance Gene ◽  
Erysiphe Pisi ◽  
Mildew Resistance ◽  
Repulsion Phase

Powdery mildew is a serious disease of pea caused by the obligate parasite Erysiphe pisi Syd. Random amplified polymorphic DNA (RAPD) analysis has emerged as a cost-effective and efficient marker system. The objective of this study was to identify RAPD markers for powdery mildew resistance gene er-1. The resistant cultivar Highlight (carrying er-1) and the susceptible cultivar Radley were crossed, and F3 plants were screened with Operon (OP) and University of British Columbia (UBC) primers, using bulked segregant analysis. A total of 416 primers were screened, of which amplicons of three Operon primers, OPO-18, OPE-16, and OPL-6, were found to be linked to er-1. OPO-181200 was linked in coupling (trans to er-1) and no recombinants were found. OPE-161600 (4 ± 2 cM) and OPL-61900 (2 ± 2 cM) were linked in repulsion (cis to er-1). The fragments OPO-181200 and OPE-161600 were sequenced and specific primers designed. The specific primer pair Sc-OPO-181200 will be useful in identifying homozygous resistant individuals in F2 and subsequent segregating generations. Sc-OPE-161600 will have greatest utility in selecting heterozygous BC\dn6 nF1 individuals in backcross breeding programs.Key words: bulked segregant analysis,Erysiphe pisi, pea, RAPD.

scholarly journals Improved Selection with Newly Identified RAPD Markers Linked to Resistance Gene to Four Pathotypes of Colletotrichum lindemuthianum in Common Bean

1999 ◽  
Vol 89 (4) ◽  
pp. 281-285 ◽  
Author(s):  
Ana Lilia Alzate-Marin ◽  
Henrique Menarim ◽  
Geraldo Assis de Carvalho ◽  
Trazilbo José de Paula ◽  
Everaldo Gonçalves de Barros ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Rapd Markers ◽  
Single Gene ◽  
Colletotrichum Lindemuthianum ◽  
Susceptible Cultivar ◽  
Coupling Phase ◽  
Repulsion Phase ◽  
Complex Locus ◽  
Two Populations

Three F2 populations derived from crosses between the resistant cultivar AB 136 and the susceptible cultivar Michelite (MiA), and one F2 population derived from a cross between AB 136 and Mexico 222 (MeA), were used to identify markers linked to anthracnose resistance genes present in cultivar AB 136. Primer OPZ04 produced a DNA band (OPZ04560) linked in coupling phase to the resistance gene for pathotype 89 (8.5 ± 0.025 cM) in one population derived from the cross MiA. In the same population, primer OPZ09 produced one band (OPZ09950) linked in repulsion phase (20.4 ± 0.014 cM) to the same resistance gene. The simultaneous use of markers in coupling and in repulsion phases allowed the identification of the three genotypic classes. In the other two populations from cross MiA, OPZ04560 was linked in coupling phase to resistance genes for pathotypes 73 (2.9 ± 0.012 cM) and 81 (2.8 ± 0.017 cM). In population MeA, OPZ04560 was linked in coupling phase (7.5 ± 0.033 cM) to resistance to pathotype 64. These data suggest that a single gene or complex locus of linked resistance genes present in cultivar AB 136 confers resistance to all four pathotypes of C. lindemuthianum.

Selection for aneuploid potato hybrids combining a low wild genome content and resistance traits from Solanum commersonii

2004 ◽  
Vol 109 (6) ◽  
pp. 1139-1146 ◽  
Author(s):  
M. Iovene ◽  
A. Barone ◽  
L. Frusciante ◽  
L. Monti ◽  
D. Carputo
Keyword(s):  
Solanum Commersonii ◽  
Genome Content ◽  
Selection For ◽  
Resistance Traits

scholarly journals PRELIMNARY RESULTS ON MARKER ASSISTED SELECTION FOR DISEASE RESISTANCE IN COMMON WHEAT (TRITICUM AESTIVUM)

1970 ◽  
Vol 62 ◽  
Author(s):  
C. Botez ◽  
Monica Iuoraş ◽  
P. Raica ◽  
N. N. Saulescu
Keyword(s):  
Triticum Aestivum ◽  
Disease Resistance ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Marker Assisted Selection ◽  
Rapd Markers ◽  
Issr Markers ◽  
Resistant Lines ◽  
Selection For

Our attempts to find RAPD markers linked with resistance genes to Septoria and Tilletia relying on co-segregation and to test the SSR markers, identified in literature, in order to select bread wheat resistant to Septoria and Tilletia are presented. Several candidate RAPD markers possibly linked with Septoria resistance genes and fewer linked with Tilletia resistance genes were identified. Some RAPD and SSR markers were specific for some resistant lines. These markers, probably, marked different resistance genes in different lines. It seems that some Septoria resistance genes came from rye genome because these genes were marked with some primers specific for rye genome. We found segregation for these markers in several lines, indicating that the analyzed lines were not completely stabilized. The ISSR markers used could not discriminate the resistant from susceptible genotypes to Septoria and Tilletia.

scholarly journals 463 PB 298 IDENTIFICATION OF RAPD MARKERS ASSOCIATED WITH CANNING QUALITY IN NAVY BEANS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 497e-497
Author(s):  
Kimberly J Walters ◽  
George L. Hosfield ◽  
James D. Kelly
Keyword(s):  
Rapd Markers ◽  
Indirect Selection ◽  
Food Science ◽  
Industry Standards ◽  
Navy Beans ◽  
The Us ◽  
Selection For ◽  
Quality Material ◽  
New Cultivars ◽  
Canning Quality

Ninety-eight percent of the navy beans (Phaseolus vulgaris) grown in the US are processed. Thus, new cultivars considered for release must meet industry standards. Canning quality behaves as a classical QTL which precludes its selection and evaluation in early generations. Such delays add a measure of inefficiency to a breeding program. Indirect selection for canning quality using molecular markers could increase efficiency. RAPD markers are more useful than RFLP's, in Phaseolus, due to a simpler protocol and a higher level of polymorphism within genetically related cultivars. Three populations of RIL's, derived from crosses between cultivars with standard and sub-standard canning quality, were screened to identify markers associated with canning quality. Material for evaluation was grown at two locations, in three replications and processed, in the Food Science Processing Lab, following industry standards. Quality traits measured were: processed texture, color and appearance. Associations of putative markers with canning quality were identified using ANOVA and Mapmaker programs

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