scholarly journals The effect of genotype on anther culture response of cultivated and wild oats

1998 ◽  
Vol 7 (3) ◽  
pp. 409-422 ◽  
Author(s):  
Elina Kiviharju ◽  
Matti Puolimatka ◽  
Marketta Saastamoinen ◽  
Simo Hovinen ◽  
Eija Pehu
Keyword(s):  
Anther Culture ◽  
Interspecific Crosses ◽  
Induction Medium ◽  
Regeneration Ability ◽  
Dichlorophenoxyacetic Acid ◽  
Wild Oat ◽  
Avena Sterilis ◽  
Naked Oat ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Anther Culture Response

Anther culture ability was tested for 44 oat (Avena sativa L.), six naked oat (A. sativa L., naked type) and 15 wild oat (Avena sterilis L.) genotypes, in addition to progeny of five intraspecific crosses of A. sativa and two interspecific crosses of A. sativa x A. sterilis. Anther culture response was affected considerably by genotype. Thirty one oat genotypes responded by callus growth on induction medium and seven of them produced embryo structures, two of the lines consistently. All naked oat genotypes produced embryo structures. Embryo production rates for the wild oat lines were comparable with those for the naked oat genotypes, and higher than for oat: 13 of the 15 genotypes tested produced embryo structures. Plant regeneration was possible only from wild oat. The regeneration ability was inherited in the progeny of the A. sativa x A. sterilis cross cv. Puhti x CAV 2648. The response of anthers of oat genotypes was inhibited by auxin on the induction medium, while naked oat, wild oat and A. sativa x A. sterilis crosses responded better on a medium containing 2,4-dichlorophenoxyacetic acid.

scholarly journals Impact of Ionic Liquids on Induction of Wheat Microspore Embryogenesis and Plant Regeneration

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 839
Author(s):  
Dorota Weigt ◽  
Idzi Siatkowski ◽  
Magdalena Magaj ◽  
Agnieszka Tomkowiak ◽  
Jerzy Nawracała
Keyword(s):  
Ionic Liquids ◽  
Plant Regeneration ◽  
Positive Impact ◽  
Microspore Embryogenesis ◽  
Green Plant ◽  
Induction Medium ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
The Impact

Ionic liquids are novel compounds with unique chemical and physical properties. They can be received based on synthetic auxins like 2,4-dichlorophenoxyacetic acid or dicamba, which are commonly used hormones in microspore embryogenesis. Nevertheless, ionic liquids have not been adapted in plant in vitro culture thus far. Therefore, we studied the impact of ionic liquids on the ability to undergo microspore embryogenesis in anther cultures of wheat. Two embryogenic and two recalcitrant genotypes were used for this study. Ten combinations of ionic liquids and 2,4-dichlorophenoxyacetic acid were added to the induction medium. In most cases, they stimulated induction of microspore embryogenesis and green plant regeneration more than a control medium supplemented with only 2,4-dichlorophenoxyacetic acid. Two treatments were the most favorable, resulting in over two times greater efficiency of microspore embryogenesis induction in comparison to the control. The effect of breaking down the genotype recalcitrance (manifested by green plant formation) was observed under the influence of 5 ionic liquids treatments. Summing up, ionic liquids had a positive impact on microspore embryogenesis induction and green plant regeneration, increasing the efficiency of these phenomena in both embryogenic and recalcitrant genotypes. Herbicidal ionic liquids can be successfully used in in vitro cultures.

Embryo induction and plant regeneration of Callerya speciosa (Fabaceae) through anther culture

2017 ◽  
Vol 65 (1) ◽  
pp. 80 ◽  
Author(s):  
Bilan Huang ◽  
Li Xu ◽  
Kelie Li ◽  
Yunlu Fu ◽  
Zhiying Li
Keyword(s):  
Anther Culture ◽  
Culture Medium ◽  
Basal Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Anther Wall ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Anther Cultures

An in vitro protocol for Callerya speciosa (Champ.) Schot regeneration through embryogenesis was developed using the anthers as the explants. The late uninucleate stage of the microspore was optimal for the anther culture of C. speciosa. Embryonic callus was induced on a MS basal medium supplemented with 4.4 µM 6-benzylaminopurine (BA) and 9.04 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Embryos were obtained on MS medium supplemented with 2.2 µM BA and 0.5 µM naphthaleneacetic acid (NAA). The highest percentage (16.7%) of embryos was achieved using the culture medium MS + 0.25 µM NAA + 1.1 µM BA. The highest percentage of embryos that developed into plants was 18.3%. However, haploid plants were not observed, which may have been due to the collection of the calli from the anther wall. The results presented here demonstrate the establishment of a highly efficient and rapid system for regenerating C. speciosa using anther cultures.

scholarly journals Regenerasi Tanaman pada Kultur Antera Beberapa Aksesi Padi Indica Toleran Aluminium

2006 ◽  
Vol 2 (1) ◽  
pp. 30
Author(s):  
Iswari S. Dewi ◽  
Bambang S. Purwoko ◽  
Hajrial Aswidinnoor ◽  
Ida H. Somantri ◽  
M. A. Chozin
Keyword(s):  
Anther Culture ◽  
Indica Rice ◽  
Aluminum Tolerance ◽  
Haploid Plant ◽  
Induction Medium ◽  
Regeneration Ability ◽  
Rice Varieties ◽  
Randomized Design ◽  
N6 Medium ◽  
Pure Lines

<p class="p1">Anther culture provides the quick route in obtaining pure lines in a single generation from either green haploid plant that may be artificially or spontaneously doubled. Indica rice known as recalcitrant genotype because of its difficulty in regenerating sufficient number of green plantlets among the regenerated plants through anther culture. Whilst, research on studying anther culture ability has to be done to assure the success of rice breeding through anther culture. The objective of this research was to determine regeneration ability of five accessions of indica rice tolerance to aluminum through application of putrescine in anther culture. Completely randomized design with 15 replications was used in this research. Treatments consisted of five accessions of aluminum tolerance indica rice, ie. CT6510-24-1-3, Grogol, Hawara Bunar, Krowal, and Sigundil. Callus induction medium based on N6 medium + 10<span class="s1">-3 </span>M putrescine, while regeneration medium based on MS + 10<span class="s1">-3 </span>M putrescine. The results indicated that culture ability is controlled by the genotype. From this research, Grogol, Krowal and Sigundil were selected as accessions having good rice anther culture ability, and therefore can be used as parents for developing new rice varieties tolerance to aluminum through anther culture.</p>

scholarly journals Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars

2012 ◽  
Vol 43 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Roberson Dibax ◽  
Giovana Bomfim de Alcantara ◽  
Marília Pereira Machado ◽  
João Carlos Bespalhok Filho ◽  
Ricardo Augusto de Oliveira
Keyword(s):  
Plant Regeneration ◽  
Histological Analysis ◽  
Induction Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Shoot Induction ◽  
In Vitro Plant Regeneration ◽  
Indirect Organogenesis ◽  
Shoot Induction Medium ◽  
2,4 Dichlorophenoxyacetic Acid

The objectives of this study were to establish appropriate conditions for obtaining plant regeneration and acclimatization of the 'RB92579' and 'RB93509' sugarcane cultivars and to elucidate the shoots origin through histological analysis. For both cultivars, obtaining shoots showed better results with the culture of explants on a callus induction medium containing 2.0mg L-1 2,4-dichlorophenoxyacetic acid, followed by cultivation on a shoot induction medium containing 0.1mg L-1 kinetin and 0.2mg L-1 benzilaminopurine. The MS medium without growth regulators proved to be appropriate for elongation and rooting of shoots and the use of the composed substrate of vermiculite + MS salts was effective for acclimatization. Histological analysis revealed that the origin of the shoots in both cultivars occurred through indirect organogenesis.

Callus formation and shoot bud differentiation in anther culture of Solanum surattense

1979 ◽  
Vol 57 (22) ◽  
pp. 2524-2527 ◽  
Author(s):  
S. Sinha ◽  
R. P. Roy ◽  
K. K. Jha
Keyword(s):  
Anther Culture ◽  
Callus Formation ◽  
Low Frequency ◽  
Basal Medium ◽  
Pollen Grains ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Cytological Examination ◽  
Shoot Bud ◽  
2,4 Dichlorophenoxyacetic Acid

In anther culture of Solatium surattense, the Murashige and Skoog's medium supplemented with 2,4-dichlorophenoxyacetic acid (2.2 mg/L), indoleacetic acid or naphthaleneacetic acid (1.9 mg/L), and kinetin (2.2 mg/L) served as “callus-producing medium.” Histological and cytological observations indicated that the callus originated from the pollen grains. Synergistic action of kinetin (5.0 mg/L) and coconut milk (15%) in basal medium was able to induce differentiation of shoot buds either from the anthers directly or from the callus. Directly differentiating buds were formed by whole shoot bud morphogenesis of pollen. They were produced at a low frequency and showed presence of well-developed radicular and plumular regions. But the buds originating from callus lacked radicular ends. Root initiation in such buds was achieved by transferring them to basal medium. Cytological examination of the androgenic plantlets revealed a chromosomal series ranging from the haploid to the hexaploid with a few aneuploids.

scholarly journals Anther culture properties of oat x wild red oat progenies and a search for RAPD markers associated with anther culture ability

2008 ◽  
Vol 13 (1-2) ◽  
pp. 151 ◽  
Author(s):  
E. KIVIHARJU ◽  
J. LAURILA ◽  
M. LEHTONEN
Keyword(s):  
Anther Culture ◽  
Avena Sativa ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Backcross Progeny ◽  
Avena Sterilis ◽  
Albino Plant ◽  
Parental Lines ◽  
Anther Culture Response ◽  
Genotype Effects

A study was carried out to improve anther culture ability of the non-responsive cultivated oat, Avena sativa L. cv. Puhti by introgressing favourable alleles from the responsive wild red oat, Avena sterilis L. acc. CAV 2648. Anther culture ability of these parental lines and F2 progenies of their cross and two backcrosses was tested. Genotype effects were significant on all anther culture traits measured. The number of anther culture derived embryo-like structures was highest in acc. CAV 2648, and the number of green regenerants from the Puhti × CAV 2648 progeny. Anther culture response was greatly reduced in backcross progeny and was least in cv. Puhti. Random amplified polymorphic DNA (RAPD) was used to test for marker associations with oat anther culture traits in a population of 38 F2 progenies. Two RAPD markers were putatively associated with improved production of green regenerants (one derived from acc. CAV 2648 and the other from cv. Puhti). One marker putatively associated with decreased albino plant regeneration (derived from acc. CAV 2648). These markers might be useful for selecting alleles for better anther culture ability among progeny of planned crosses. In addition, three markers, derived from acc. CAV 2648, were putatively associated with decreased anther culture response rates.;

scholarly journals Somatic Embryogenesis and Regeneration from Cotyledon Explants of Six Squash Cultivars

HortScience ◽  
1995 ◽  
Vol 30 (6) ◽  
pp. 1295-1297 ◽  
Author(s):  
Carol Gonsalves ◽  
Baodi Xue ◽  
Dennis Gonsalves
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Induction Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Plantlet Production ◽  
Summer Squash ◽  
Napthaleneacetic Acid ◽  
Relative Production ◽  
2,4 Dichlorophenoxyacetic Acid

Six summer squash (Cucurbita pepo L.) cultivars were regenerated via somatic embryogenesis using cotyledons excised from germinated or nongerminated seeds. Genotypes included were zucchini, commercial F1 hybrids, `President', `Seneca Zucchini', `Jade'; the noncommercial inbred line `Caserta Inbred 557311'; and two yellow squash hybrids `Dixie' and `Seneca Butterbar'. Somatic embryogenesis was initiated in induction medium containing 22.62 μm 2, 4-D, and embryos were germinated in maturation medium containing 0.27 μm NAA and 0.23 μm kinetin. Plants were elongated and rooted on basal medium without hormones. All media contained carbenicillin at 500 mg·liter–1. Sixty-one percent of the `Seneca Butterbar' cotyledons produced somatic embryos when kept on induction medium for 10 weeks. Overall, 7% of the initial explants produced plantlets, and regeneration efficiency was calculated as 0.3 plantlets per initial explant. The relative production of plants from cotyledons that were kept on induction medium for different time periods were determined for `Caserta Inbred 557311' and `Seneca Zucchini'. All cotyledons produced somatic embryos after 11 to 17 weeks on induction medium. However, plantlet production was optimal with explants kept on induction medium for 13 weeks for `Seneca Zucchini' and for 15 weeks for `Caserta Inbred 557311', producing an average of 4.5 and 9.3 plants per explant, respectively, from 90% to 70% of the explants. We recovered plants from all six cultivars; thus, our regeneration protocol may be applicable to other genotypes. The high percentage of regenerants obtained indicates that the regeneration method is efficient enough to be adapted successfully to squash transformation experiments. Chemical names used: α-carboxybenzylpenicillin (carbenicillin); 2,4-dichlorophenoxyacetic acid (2,4-D); 6-furfurylaminopurine (kinetin); α-napthaleneacetic acid (NAA).

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