scholarly journals Accurate Quantification of Expression of Transgenes Marked with Restriction Endonuclease Site Polymorphisms by RT-PCR

BioTechniques ◽  
1998 ◽  
Vol 25 (4) ◽  
pp. 558-562 ◽  
Author(s):  
Shuyu Li ◽  
Julia B. George-Raizen ◽  
Robert E. Hammer ◽  
William T. Garrard
Keyword(s):  
Restriction Endonuclease ◽  
Rt Pcr ◽  
Restriction Endonuclease Site ◽  
Accurate Quantification

scholarly journals Classification of medically important clostridia using restriction endonuclease site differences of PCR-amplified 16S rDNA

1991 ◽  
Vol 137 (11) ◽  
pp. 2673-2679 ◽  
Author(s):  
V. Gurtler ◽  
V. A. Wilson ◽  
B. C. Mayall
Keyword(s):  
Restriction Endonuclease ◽  
16S Rdna ◽  
Restriction Endonuclease Site

Identification of ATM mutations using extended RT-PCR and restriction endonuclease fingerprinting, and elucidation of the repertoire of A-T mutations in Israel

1998 ◽  
Vol 11 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Shlomit Gilad ◽  
Rami Khosravi ◽  
Reli Harnik ◽  
Yael Ziv ◽  
Dganit Shkedy ◽  
...  
Keyword(s):  
Restriction Endonuclease ◽  
Rt Pcr

Cloning and identification of the product of the dnaE gene of Escherichia coli

1982 ◽  
Vol 152 (1) ◽  
pp. 351-356
Author(s):  
M M Welch ◽  
C S McHenry
Keyword(s):  
Escherichia Coli ◽  
Restriction Endonuclease ◽  
Dna Polymerase ◽  
Alpha Subunit ◽  
Temperature Sensitive ◽  
Restriction Map ◽  
Dna Polymerase Iii ◽  
Restriction Endonuclease Site ◽  
E Coli ◽  
A Site

We successively subcloned the dnaE gene of Escherichia coli into pBR322, resulting in a plasmid that contains 4.6 kilobases of E. coli DNA. This plasmid can complement a dnaE temperature-sensitive mutation. A restriction map of the dnaE gene and the surrounding 10.7-kilobase region of the E. coli chromosome was determined. A unique HindIII restriction endonuclease site within the cloned segment of DNA was identified as a site required for expression of the dnaE gene. By using the maxicell plasmid-directed protein synthesizing system, we demonstrated that dnaE codes for the alpha subunit of DNA polymerase III.

scholarly journals Pathotyping of Local Isolates Newcastle Disease Virus from Field Specimens by RT-PCR and Restriction Endonuclease Analysis

2015 ◽  
Vol 14 ◽  
pp. 85-90 ◽  
Author(s):  
Aris Haryanto ◽  
Medania Purwaningrum ◽  
Sutopo Verawati ◽  
Sri Handayani Irianingsih ◽  
Nastiti Wijayanti
Keyword(s):  
Newcastle Disease Virus ◽  
Restriction Endonuclease ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Restriction Endonuclease Analysis ◽  
Rt Pcr ◽  
Endonuclease Analysis

scholarly journals Gene Detection and Discrimination of Pestivirus Strains by RT-PCR and Restriction Endonuclease Analysis

1997 ◽  
Vol 50 (11) ◽  
pp. 639-644 ◽  
Author(s):  
Osamu YAMAGUCHI ◽  
Yoshihiro SAKODA ◽  
Mitsuo SATO ◽  
Shigeyuki NAKAMURA ◽  
Akio FUKUSHO
Keyword(s):  
Restriction Endonuclease ◽  
Restriction Endonuclease Analysis ◽  
Rt Pcr ◽  
Gene Detection ◽  
Endonuclease Analysis

Characterization of a Polymerase Chain Reaction–Based Approach for the Simultaneous Detection of Multiple Animal-Derived Materials in Animal Feed

2003 ◽  
Vol 66 (6) ◽  
pp. 1085-1089 ◽  
Author(s):  
MICHAEL J. MYERS ◽  
HAILE F. YANCY ◽  
DOROTHY E. FARRELL
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Endonuclease ◽  
Blood Meal ◽  
Animal Feed ◽  
Enzymatic Digestion ◽  
Chain Reaction ◽  
Restriction Endonuclease Site ◽  
Dog Food ◽  
Polymerase Chain ◽  
Pcr Primer

In this study, a polymerase chain reaction (PCR) primer set capable of amplifying a mitochondrial DNA segment of multiple species (cattle, sheep, goats, deer, and elk) whose rendered remains are prohibited from being fed to ruminants was characterized. However, the primer set also amplifies DNA derived from the rendered remains of pigs and horses, which are exempt from the feed ban. PCR amplicons derived from pig DNA have a restriction endonuclease site recognized by Hinf1, while the horse DNA–derived amplicon has a unique restriction endonuclease site recognized by HypCH4III. This “universal” PCR primer produced an amplicon with DNA extracted from dairy feed containing either bovine meat and bone meal or pig blood meal. Enzymatic digestion of the PCR amplicons from these feed samples with Hinf1 resulted in cleavage products only from samples containing pig blood meal. However, Hinf1 digestion of these amplicons was not complete. Further analysis of the pig blood meal with primers specific for bovine or porcine DNA demonstrated the presence of both bovine- and porcine-derived DNA. Enzymatic digestion confirmed these findings. Additional testing was conducted with dry dog food samples labeled as containing either lamb, chicken, turkey, or chicken and fish. The universal PCR primer produced an amplicon only for the dog food containing lamb meal. This paper is the first to describe a simplified approach for the detection of the prohibited species of concern in the feed ban.

Genomic arrangement of repeated PS700 elements in the nematode Panagrellus silusiae

Genome ◽  
1990 ◽  
Vol 33 (2) ◽  
pp. 164-169
Author(s):  
M. A. Retterath ◽  
J. J. Pasternak
Keyword(s):  
Nucleotide Sequence ◽  
Restriction Endonuclease ◽  
Repetitive Dna ◽  
Genomic Dna ◽  
Restriction Endonuclease Site ◽  
Site Mapping ◽  
Genomic Arrangement ◽  
Nucleotide Sequence Variation ◽  
Ethidium Bromide Staining ◽  
Novel Variant

When genomic DNA from the free-living nematode Panagrellus silusiae is digested with the restriction endonuclease BamHI and separated by electrophoresis, a band in the 700 base pair size range is evident after ethidium bromide staining. One of the 0.7-kilobase fragments (PS700-1) was characterized and found to be a member of a moderately repetitive DNA family (T. Warren and J.J. Pasternak. 1988. Nucleic Acids Res. 16: 10 833 – 10 847). In the current study, DNA sequence analyses of three independently isolated copies of the PS700 DNA family showed the same nucleotide sequence and >98% similarity to PS700-1. Four EMBL-4 bacteriophage clones were isolated from a Panagrellus genomic DNA library with PS700-1 as the probe and were analyzed by restriction endonuclease site mapping and Southern blot DNA hybridization. These clones contain 31 copies of the PS700 DNA family. In each case, the units are arranged in head-to-tail arrays. One of the EMBL-4 clones contains copies of a novel variant of the PS700 elements. The maintenance of both nucleotide sequence and restriction endonuclease restriction site homogeneity among members of the dispersed PS700 DNA family may denote a functional role for these sequences.Key words: nematode, Panagrellus, repetitive DNA organization, nucleotide sequence variation.

Accurate quantification of the mRNA of NMDAR1 splice variants measured by competitive RT-PCR

1999 ◽  
Vol 4 (1) ◽  
pp. 69-81 ◽  
Author(s):  
Anett Winkler ◽  
Beatrice Mahal ◽  
Walter Zieglgänsberger ◽  
Rainer Spanagel
Keyword(s):  
Splice Variants ◽  
Rt Pcr ◽  
Accurate Quantification
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