Genomic arrangement of repeated PS700 elements in the nematode Panagrellus silusiae

Genome ◽  
1990 ◽  
Vol 33 (2) ◽  
pp. 164-169
Author(s):  
M. A. Retterath ◽  
J. J. Pasternak
Keyword(s):  
Nucleotide Sequence ◽  
Restriction Endonuclease ◽  
Repetitive Dna ◽  
Genomic Dna ◽  
Restriction Endonuclease Site ◽  
Site Mapping ◽  
Genomic Arrangement ◽  
Nucleotide Sequence Variation ◽  
Ethidium Bromide Staining ◽  
Novel Variant

When genomic DNA from the free-living nematode Panagrellus silusiae is digested with the restriction endonuclease BamHI and separated by electrophoresis, a band in the 700 base pair size range is evident after ethidium bromide staining. One of the 0.7-kilobase fragments (PS700-1) was characterized and found to be a member of a moderately repetitive DNA family (T. Warren and J.J. Pasternak. 1988. Nucleic Acids Res. 16: 10 833 – 10 847). In the current study, DNA sequence analyses of three independently isolated copies of the PS700 DNA family showed the same nucleotide sequence and >98% similarity to PS700-1. Four EMBL-4 bacteriophage clones were isolated from a Panagrellus genomic DNA library with PS700-1 as the probe and were analyzed by restriction endonuclease site mapping and Southern blot DNA hybridization. These clones contain 31 copies of the PS700 DNA family. In each case, the units are arranged in head-to-tail arrays. One of the EMBL-4 clones contains copies of a novel variant of the PS700 elements. The maintenance of both nucleotide sequence and restriction endonuclease restriction site homogeneity among members of the dispersed PS700 DNA family may denote a functional role for these sequences.Key words: nematode, Panagrellus, repetitive DNA organization, nucleotide sequence variation.

scholarly journals Structure of murine complement component C3. I. Nucleotide sequence of cloned complementary and genomic DNA coding for the beta chain.

1984 ◽  
Vol 259 (22) ◽  
pp. 13851-13856 ◽  
Author(s):  
A Lundwall ◽  
R A Wetsel ◽  
H Domdey ◽  
B F Tack ◽  
G H Fey
Keyword(s):  
Nucleotide Sequence ◽  
Genomic Dna ◽  
Complement Component ◽  
Beta Chain ◽  
Dna Coding ◽  
Complement Component C3

scholarly journals Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1447
Author(s):  
Ishaku L. Haruna ◽  
Yunhai Li ◽  
Ugonna J. Ekegbu ◽  
Hamed Amirpour-Najafabadi ◽  
Huitong Zhou ◽  
...  
Keyword(s):  
Fatty Acid ◽  
New Zealand ◽  
Nucleotide Sequence ◽  
Milk Fat ◽  
Linear Mixed Effect Model ◽  
Polymorphism Analysis ◽  
Mixed Effect ◽  
Myostatin Gene ◽  
Holstein Friesian ◽  
Nucleotide Sequence Variation

The myostatin gene (MSTN), which encodes the protein myostatin, is pleiotropic, and its expression has been associated with both increased and decreased adipogenesis and increased skeletal muscle mass in animals. In this study, the polymerase chain reaction, coupled with single strand conformation polymorphism analysis, was utilized to reveal nucleotide sequence variation in bovine MSTN in 410 New Zealand (NZ) Holstein-Friesian × Jersey (HF × J)-cross cows. These cows ranged from 3 to 9 years of age and over the time studied, produced an average 22.53 ± 2.18 L of milk per day, with an average milk fat content of 4.94 ± 0.17% and average milk protein content of 4.03 ± 0.10%. Analysis of a 406-bp amplicon from the intron 1 region, revealed five nucleotide sequence variants (A–E) that contained seven nucleotide substitutions. Using general linear mixed-effect model analyses the AD genotype was associated with reduced C10:0, C12:0, and C12:1 levels when compared to levels in cows with the AA genotype. These associations in NZ HF × J cross cows are novel, and they suggest that this variation in bovine MSTN could be explored for increasing the amount of milk unsaturated fatty acid and decreasing the amount of saturated fatty acid.

scholarly journals Cytochrome b Nucleotide Sequence Variation Among the Atlantic Alcidae

Hereditas ◽  
2004 ◽  
Vol 119 (3) ◽  
pp. 245-252 ◽  
Author(s):  
V. L. Friesen ◽  
W. A. Montevecchi ◽  
W. S. Davidson
Keyword(s):  
Nucleotide Sequence ◽  
Cytochrome B ◽  
Sequence Variation ◽  
Nucleotide Sequence Variation

Genomic variability within laboratory and wild subspecies of Heligmosomoides polygyrus

1994 ◽  
Vol 68 (2) ◽  
pp. 93-96 ◽  
Author(s):  
M.A. Abu-Madi ◽  
A.P. Reid ◽  
J.W. Lewis ◽  
W.M. Hominick
Keyword(s):  
Restriction Endonuclease ◽  
Ribosomal Dna ◽  
Dna Hybridization ◽  
Genomic Dna ◽  
Dna Probes ◽  
Heligmosomoides Polygyrus ◽  
Banding Patterns ◽  
Genomic Variability

AbstractGenomic DNA extracted from laboratory and wild subspecies of the trichostrongyle nematode Heligmosomoides polygyrus were compared using RFLP and DNA/DNA hybridization techniques. Eight restriction endonuclease digests of the genomic DNA of the two subspecies were hybridized with heterologous ribosomal DNA probes and the total radio-isotope labelled DNA of the laboratory subspecies. DNA hybridization of the two subspecies of H. polygyrus yielded different banding patterns when probed with the rDNA clones in Pvu II digests and when total genomic DNA was used as the probe in Hind III and Pvu II digests. The remaining hybridization profiles of both subspecies were identical.

scholarly journals Nucleotide Sequence Variation in the Ribosomal Internal Transcribed Spacer Regions of Cultivated (Cultivars) and Field-collected Thalli of Porphyra yezoensis

1999 ◽  
Vol 65 (5) ◽  
pp. 788-789 ◽  
Author(s):  
Yuzuru Mizukami ◽  
Hitoshi Kito ◽  
Yoshio Kaminishi ◽  
Noboru Murase ◽  
Masahiko Kunimoto
Keyword(s):  
Nucleotide Sequence ◽  
Internal Transcribed Spacer ◽  
Sequence Variation ◽  
Porphyra Yezoensis ◽  
Internal Transcribed Spacer Regions ◽  
Nucleotide Sequence Variation

scholarly journals Nucleotide sequence variation associated with β-amylase deficiency in the sweet potato Ipomoea batatas (L.) Lam

2009 ◽  
Vol 59 (3) ◽  
pp. 209-216 ◽  
Author(s):  
Nadia Anwar ◽  
Akira Kikuchi ◽  
Toru Kumagai ◽  
Kazuo N. Watanabe
Keyword(s):  
Nucleotide Sequence ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Sequence Variation ◽  
Nucleotide Sequence Variation

Molecular and cytogenetic analysis of repetitive DNA in pea (Pisum sativum L.)

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 716-728 ◽  
Author(s):  
Pavel Neumann ◽  
Marcela Nouzová ◽  
Jirí Macas
Keyword(s):  
Pisum Sativum ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Genomic Dna ◽  
Tandem Repeats ◽  
Genomic Library ◽  
Chromosome Morphology ◽  
Plant Genome ◽  
Genomic Repeats ◽  
Dispersed Repeats

A set of pea DNA sequences representing the most abundant genomic repeats was obtained by combining several approaches. Dispersed repeats were isolated by screening a short-insert genomic library using genomic DNA as a probe. Thirty-two clones ranging from 149 to 2961 bp in size and from 1000 to 39 000/1C in their copy number were sequenced and further characterized. Fourteen clones were identified as retrotransposon-like sequences, based on their homologies to known elements. Fluorescence in situ hybridization using clones of reverse transcriptase and integrase coding sequences as probes revealed that corresponding retroelements were scattered along all pea chromosomes. Two novel families of tandem repeats, named PisTR-A and PisTR-B, were isolated by screening a genomic DNA library with Cot-1 DNA and by employing genomic self-priming PCR, respectively. PisTR-A repeats are 211–212 bp long, their abundance is 2 × 104 copies/1C, and they are partially clustered in a secondary constriction of one chromosome pair with the rest of their copies dispersed on all chromosomes. PisTR-B sequences are of similar abundance (104 copies/1C) but differ from the "A" family in their monomer length (50 bp), high A/T content, and chromosomal localization in a limited number of discrete bands. These bands are located mainly in (sub)telomeric and pericentromeric regions, and their patterns, together with chromosome morphology, allow discrimination of all chromosome types within the pea karyotype. Whereas both tandem repeat families are mostly specific to the genus Pisum, many of the dispersed repeats were detected in other legume species, mainly those in the genus Vicia.Key words: repetitive DNA, plant genome, retroelements, satellite DNA, Pisum sativum.

Sign in / Sign up

Export Citation Format

Share Document