scholarly journals Digital holographic microscopy: a noninvasive method to analyze the formation of spheroids

BioTechniques ◽  
2021 ◽  
Author(s):  
Zahra El-Schich
Keyword(s):  
Digital Holographic Microscopy ◽  
Noninvasive Method ◽  
Cell Spheroids ◽  
A Cell ◽  
Cell Culture Protocol ◽  
Holographic Microscopy ◽  
Size And Morphology ◽  
In Vitro Cell Cultures ◽  
Culture Protocol

Digital holographic (DH) microscopy is a unique noninvasive method to analyze living cells. With DH microscopy, in vitro cell cultures can be imaged in 2D and pseudo-3D and measurements of size and morphology of the cells are provided. Here, a description of a novel methodology utilizing DH microscopy for the analysis of spheroids is presented. A cell culture protocol is introduced and morphological parameters of cell spheroids as measured by DH microscopy are presented. The study confirms the use of DH microscopy for the analysis of cell spheroids. In the future, organoids can be analyzed with DH microscopy, and it can also be used for drug response and cell death analyses.

Quantitative evaluation of morphological changes in activated platelets in vitro using digital holographic microscopy

Micron ◽  
2018 ◽  
Vol 113 ◽  
pp. 1-9 ◽  
Author(s):  
Yutaka Kitamura ◽  
Kazushige Isobe ◽  
Hideo Kawabata ◽  
Tetsuhiro Tsujino ◽  
Taisuke Watanabe ◽  
...  
Keyword(s):  
Quantitative Evaluation ◽  
Morphological Changes ◽  
Digital Holographic Microscopy ◽  
Activated Platelets ◽  
Holographic Microscopy

scholarly journals Quantitative Stain-Free and Continuous Multimodal Monitoring of Wound Healing In Vitro with Digital Holographic Microscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107317 ◽  
Author(s):  
Dominik Bettenworth ◽  
Philipp Lenz ◽  
Philipp Krausewitz ◽  
Markus Brückner ◽  
Steffi Ketelhut ◽  
...  
Keyword(s):  
Wound Healing ◽  
Digital Holographic Microscopy ◽  
Multimodal Monitoring ◽  
Holographic Microscopy

3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

2014 ◽  
Author(s):  
F. Merola ◽  
L. Miccio ◽  
P. Memmolo ◽  
G. Di Caprio ◽  
G. Coppola ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Digital Holographic Microscopy ◽  
3D Manipulation ◽  
Holographic Microscopy

Digital Holographic Microscopy Applied to Diffraction Tomography of a Cell Refractive Index

2006 ◽  
Author(s):  
Florian Charrière ◽  
Tristan Colomb ◽  
Christian Depeursinge ◽  
Pierre Marquet ◽  
Etienne Cuche
Keyword(s):  
Refractive Index ◽  
Digital Holographic Microscopy ◽  
Diffraction Tomography ◽  
A Cell ◽  
Holographic Microscopy

In vitro monitoring of photoinduced necrosis in HeLa cells using digital holographic microscopy and machine learning

2020 ◽  
Vol 37 (2) ◽  
pp. 346
Author(s):  
A. V. Belashov ◽  
A. A. Zhikhoreva ◽  
T. N. Belyaeva ◽  
E. S. Kornilova ◽  
A. V. Salova ◽  
...  
Keyword(s):  
Machine Learning ◽  
Hela Cells ◽  
Digital Holographic Microscopy ◽  
Holographic Microscopy

Quantification of inflammation in colonic tissue sections and wound healing in-vitro with digital holographic microscopy

2013 ◽  
Author(s):  
Dominik Bettenworth ◽  
Philipp Lenz ◽  
Philipp Krausewitz ◽  
Markus Brückner ◽  
Steffi Ketelhut ◽  
...  
Keyword(s):  
Wound Healing ◽  
Digital Holographic Microscopy ◽  
Colonic Tissue ◽  
Tissue Sections ◽  
Holographic Microscopy

Label-Free Quantitative In Vitro Live Cell Imaging with Digital Holographic Microscopy

2019 ◽  
Author(s):  
B. Kemper ◽  
A. Bauwens ◽  
D. Bettenworth ◽  
M. Götte ◽  
B. Greve ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Digital Holographic Microscopy ◽  
Label Free ◽  
Holographic Microscopy
Sign in / Sign up

Export Citation Format

Share Document