A Pan-Cancer Analysis of the Role of Selenoprotein P mRNA in Tumorigenesis

Abstract Background Tumor-associated macrophages (TAMs) are abundant in the tumor microenvironment (TME). However, their contribution to the immunosuppressive status of the TME remains unclear. Methods We integrated single-cell sequencing and transcriptome data from different tumor types to uncover the molecular features of TAMs. In vitro experiments and prospective clinical tests confirmed the results of these analysis. Results We first detected intra- and inter-tumoral heterogeneities between TAM subpopulations and their functions, with CD86+ TAMs playing a crucial role in tumor progression. Next, we focused on the ligand-receptor interactions between TAMs and tumor cells in different TME phenotypes and discovered that aberrant expressions of six hub genes, including FLI1, are involved in this process. A TAM-tumor cell co-culture experiment proved that FLI1 was involved in tumor cell invasion, and FLI1 also showed a unique pattern in patients. Finally, TAMs were discovered to communicate with immune and stromal cells. Conclusion We determined the role of TAMs in the TME by focusing on their communication pattern with other TME components. Additionally, the screening of hub genes revealed potential therapeutic targets.

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Multidimensional Analysis of the Role of Charged Multivesicular Body Protein 7 in Pan-Cancer

International Journal of General Medicine ◽

10.2147/ijgm.s337876 ◽

2021 ◽

Vol Volume 14 ◽

pp. 7907-7923

Author(s):

Yu Guo ◽

Jian Shi ◽

Zeyun Zhao ◽

Min Wang

Keyword(s):

Multivesicular Body ◽

Multidimensional Analysis ◽

Body Protein ◽

Pan Cancer

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Prognostic value and immunological role of PDCD1 gene in pan-cancer

International Immunopharmacology ◽

10.1016/j.intimp.2020.107080 ◽

2020 ◽

Vol 89 ◽

pp. 107080

Author(s):

Yandong Miao ◽

Jiangtao Wang ◽

Qiutian Li ◽

Wuxia Quan ◽

Yingying Wang ◽

...

Keyword(s):

Prognostic Value ◽

Pan Cancer

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Evidence for intestinal release of absorbed selenium in a form with high hepatic extraction

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1992.262.5.g854 ◽

1992 ◽

Vol 262 (5) ◽

pp. G854-G858 ◽

Cited By ~ 2

Author(s):

T. Kato ◽

R. Read ◽

J. Rozga ◽

R. F. Burk

Keyword(s):

Portal Vein ◽

Gel Filtration ◽

Systemic Circulation ◽

Selenoprotein P ◽

Intragastric Administration ◽

Hepatic Extraction ◽

Extraction Capacity ◽

Selenium Uptake ◽

Better Than

Selenium is readily absorbed from the gastrointestinal tract and utilized for synthesis of selenoproteins. Roles of intestine, liver, and selenoprotein P in this process were evaluated. Rats were given 75Se-selenite by stomach tube, and distribution of 75Se was followed for 3 h. A high portal vein plasma-to-hepatic vein plasma ratio of 75Se 15 min after 75Se administration and earlier uptake by liver than by other tissues indicated avid hepatic extraction of absorbed selenium from portal vein blood. The results of gel filtration of plasma taken 15 min after 75Se administration suggested that the 75Se was in the form of small molecules with some affinity for protein. Immunoprecipitation studies using plasma indicated that 75Se began to appear in selenoprotein P between 15 and 30 min after intragastric administration. To evaluate the role of the liver in the fate of absorbed selenium, rats with portacaval shunts, in which absorbed selenium bypasses the liver, were compared with sham-operated rats. After intragastric administration of selenium, uptake by the liver and incorporation into selenoprotein P were diminished in rats with portacaval shunts but kidney uptake and urinary excretion were increased. This suggests that hepatic extraction of absorbed selenium from portal vein blood decreases its entrance into the systemic circulation. The results of this study indicate that intestine releases absorbed selenium into portal blood in a small-molecule form, designated A-Se, which is highly extracted by the liver. The liver takes up A-Se better than other tissues because of a high extraction capacity and the fact that it is the first organ through which the blood from the intestine passes.

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Gene disruption discloses role of selenoprotein P in selenium delivery to target tissues

Biochemical Journal ◽

10.1042/bj20021853 ◽

2003 ◽

Vol 370 (2) ◽

pp. 397-402 ◽

Cited By ~ 287

Author(s):

Lutz SCHOMBURG ◽

Ulrich SCHWEIZER ◽

Bettina HOLTMANN ◽

Leopold FLOHÉ ◽

Michael SENDTNER ◽

...

Keyword(s):

Knockout Mice ◽

Gene Disruption ◽

Pivotal Role ◽

Selenoprotein P ◽

Selenium Content ◽

Antioxidant Defence

Selenoprotein P (SePP), the major selenoprotein in plasma, has been implicated in selenium transport, selenium detoxification or antioxidant defence. We generated SePP-knockout mice that were viable, but exhibited reduced growth and developed ataxia. Selenium content was elevated in liver, but low in plasma and other tissues, and selenoenzyme activities changed accordingly. Our data reveal that SePP plays a pivotal role in delivering hepatic selenium to target tissues.

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Expression of GRINA Correlates with Prognosis in Human Cancers: A Pan-cancer Analysis

10.1101/2021.05.13.444089 ◽

2021 ◽

Author(s):

S. M. Riazul Islam ◽

Subbroto Kumar Saha ◽

Shaker El-Sappagh ◽

Faisal Tariq ◽

Joydeep Das ◽

...

Keyword(s):

Patient Survival ◽

Prognostic Significance ◽

Functional Enrichment ◽

Regulatory Pathways ◽

Human Cancers ◽

Upstream Regulator ◽

Mrna And Protein Expression ◽

Prostate Cancers ◽

Pan Cancer

GRINA is an emerging target for cancer therapy. However, the role of GRINA expression and its correlation with cancer patient survival has not been comprehensively studied. Here, we found that mRNA and protein expression of GRINA was upregulated in breast, colon, gastric, and prostate cancers and negatively correlated with patient survival. Also, the upregulation of GRINA expression is associated with hypomethylation of its promoter region. Our GRINA-miRNAs network analysis revealed potential regulatory miRNAs regulating the GRINA expression and its downstream pathways. Next, functional enrichment and pathway analysis of genes commonly co-express with GRINA in breast, colon, gastric, and prostate cancers revealed GRINA regulatory pathways. Concurrently, our upstream regulator analysis revealed possible kinases, transcription factors, and proteins that may potentially regulate GRINA . Overall, this study demonstrates the prognostic significance of GRINA expression and identifies potential regulatory mechanisms, which might have significant implications in targeted therapies for human cancers.

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A pan-cancer analysis revealed the role of the SLC16 family in cancer

Channels ◽

10.1080/19336950.2021.1965422 ◽

2021 ◽

Vol 15 (1) ◽

pp. 528-540 ◽

Cited By ~ 1

Author(s):

Jun Li ◽

Jiaheng Xie ◽

Dan Wu ◽

Liang Chen ◽

Zetian Gong ◽

...

Keyword(s):

Pan Cancer

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Pan-Cancer Analysis of the Carcinogenic Effect of Nuclear Respiratory Factor-1 (NRF1) in Human Tumors

10.21203/rs.3.rs-970754/v1 ◽

2021 ◽

Author(s):

Yong Jiang ◽

Yaodan Chang ◽

Jiahui Sun ◽

Yanping Sun ◽

Chunjuan Yang ◽

...

Keyword(s):

Cell Biology ◽

Potential Role ◽

Animal Experiments ◽

Tumor Patients ◽

Carcinogenic Effect ◽

Nuclear Respiratory Factor ◽

Nuclear Respiratory Factor 1 ◽

Pan Cancer ◽

Pathogenic Effects

Abstract Despite there are many cellular or animal experiments that support a link between NRF1 and cancer, there is currently no pan-cancer analysis available. Therefore, the source of data is TCGA and GEO, we explored the potential role of NRF1 in 33 kinds of tumors. The expression of NRF1 is significantly increased in most tumors, and the expression of NRF1 is correlated to the prognosis of tumor patients. We discovered that the expression level of NRF1 in PAAD was correlated to immune infiltration. The methylation of NRF1 increased significantly in KIRC, PAAD, ACC. In addition, RNA metabolic pathway or cell biology-related functions participate in the functional mechanism of NRF1.Our research provides a comprehensive explanation of the pathogenic effects of NRF1 in related tumors.

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