scholarly journals Multidimensional Analysis of the Role of Charged Multivesicular Body Protein 7 in Pan-Cancer

2021 ◽  
Vol Volume 14 ◽  
pp. 7907-7923
Author(s):  
Yu Guo ◽  
Jian Shi ◽  
Zeyun Zhao ◽  
Min Wang
Keyword(s):  
Multivesicular Body ◽  
Multidimensional Analysis ◽  
Body Protein ◽  
Pan Cancer

scholarly journals The role of charged multivesicular body protein 5 in programmed cell death in leukemic cells

2013 ◽  
Vol 45 (5) ◽  
pp. 383-390 ◽  
Author(s):  
H. Wang ◽  
J. Liu ◽  
F. Wang ◽  
M. Chen ◽  
Z. Xiao ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Multivesicular Body ◽  
Leukemic Cells ◽  
Body Protein

scholarly journals Prognostic and immunological role of Ras-related protein Rap1b in pan-cancer

Bioengineered ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 4828-4840
Author(s):  
Guoliang Cui ◽  
Can Wang ◽  
Zhenyan Lin ◽  
Xiaoke Feng ◽  
Muxin Wei ◽  
...  
Keyword(s):  
Related Protein ◽  
Pan Cancer

scholarly journals Low level of plasminogen increases risk for mortality in COVID-19 patients

2021 ◽  
Vol 12 (8) ◽  
Author(s):  
David Della-Morte ◽  
Francesca Pacifici ◽  
Camillo Ricordi ◽  
Renato Massoud ◽  
Valentina Rovella ◽  
...  
Keyword(s):  
Fasting Blood Glucose ◽  
Multidimensional Analysis ◽  
Strongly Correlated ◽  
Analysis Model ◽  
Inflammatory Parameters ◽  
Number Of Patients ◽  
Caucasian Patients ◽  
Low Levels ◽  
Organ Dysfunctions

AbstractThe pathophysiology of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and especially of its complications is still not fully understood. In fact, a very high number of patients with COVID-19 die because of thromboembolic causes. A role of plasminogen, as precursor of fibrinolysis, has been hypothesized. In this study, we aimed to investigate the association between plasminogen levels and COVID-19-related outcomes in a population of 55 infected Caucasian patients (mean age: 69.8 ± 14.3, 41.8% female). Low levels of plasminogen were significantly associated with inflammatory markers (CRP, PCT, and IL-6), markers of coagulation (D-dimer, INR, and APTT), and markers of organ dysfunctions (high fasting blood glucose and decrease in the glomerular filtration rate). A multidimensional analysis model, including the correlation of the expression of coagulation with inflammatory parameters, indicated that plasminogen tended to cluster together with IL-6, hence suggesting a common pathway of activation during disease’s complication. Moreover, low levels of plasminogen strongly correlated with mortality in COVID-19 patients even after multiple adjustments for presence of confounding. These data suggest that plasminogen may play a pivotal role in controlling the complex mechanisms beyond the COVID-19 complications, and may be useful both as biomarker for prognosis and for therapeutic target against this extremely aggressive infection.

scholarly journals Pan-cancer analysis reveals tumor-associated macrophage communication in the tumor microenvironment

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Linbang Wang ◽  
Tao He ◽  
Jingkun Liu ◽  
Jiaojiao Tai ◽  
Bing Wang ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Tumor Cell ◽  
Hub Genes ◽  
Tumor Associated Macrophage ◽  
Molecular Features ◽  
Receptor Interactions ◽  
Tumor Types ◽  
Pan Cancer

Abstract Background Tumor-associated macrophages (TAMs) are abundant in the tumor microenvironment (TME). However, their contribution to the immunosuppressive status of the TME remains unclear. Methods We integrated single-cell sequencing and transcriptome data from different tumor types to uncover the molecular features of TAMs. In vitro experiments and prospective clinical tests confirmed the results of these analysis. Results We first detected intra- and inter-tumoral heterogeneities between TAM subpopulations and their functions, with CD86+ TAMs playing a crucial role in tumor progression. Next, we focused on the ligand-receptor interactions between TAMs and tumor cells in different TME phenotypes and discovered that aberrant expressions of six hub genes, including FLI1, are involved in this process. A TAM-tumor cell co-culture experiment proved that FLI1 was involved in tumor cell invasion, and FLI1 also showed a unique pattern in patients. Finally, TAMs were discovered to communicate with immune and stromal cells. Conclusion We determined the role of TAMs in the TME by focusing on their communication pattern with other TME components. Additionally, the screening of hub genes revealed potential therapeutic targets.

scholarly journals A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yuting Guan ◽  
Xiujie Liang ◽  
Ziyuan Ma ◽  
Hailong Hu ◽  
Hongbo Liu ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Association Studies ◽  
Genetic Locus ◽  
Human Kidney ◽  
Multivesicular Body ◽  
Chromatin Accessibility ◽  
Disease Genes ◽  
Genome Wide Association Studies ◽  
Disease Development ◽  
Body Protein

AbstractGenome-wide association studies (GWAS) have identified loci for kidney disease, but the causal variants, genes, and pathways remain unknown. Here we identify two kidney disease genes Dipeptidase 1 (DPEP1) and Charged Multivesicular Body Protein 1 A (CHMP1A) via the triangulation of kidney function GWAS, human kidney expression, and methylation quantitative trait loci. Using single-cell chromatin accessibility and genome editing, we fine map the region that controls the expression of both genes. Mouse genetic models demonstrate the causal roles of both genes in kidney disease. Cellular studies indicate that both Dpep1 and Chmp1a are important regulators of a single pathway, ferroptosis and lead to kidney disease development via altering cellular iron trafficking.

CHMP1 is a novel nuclear matrix protein affecting chromatin structure and cell-cycle progression

2001 ◽  
Vol 114 (13) ◽  
pp. 2383-2393 ◽  
Author(s):  
Daniel R. Stauffer ◽  
Tiffani L. Howard ◽  
Thihan Nyun ◽  
Stanley M. Hollenberg
Keyword(s):  
Gene Silencing ◽  
Nuclear Matrix ◽  
Cell Cycle Progression ◽  
Matrix Protein ◽  
Multivesicular Body ◽  
Polycomb Group ◽  
Condensed Chromatin ◽  
Stable Gene ◽  
Body Protein ◽  
Reading Frame

The Polycomb-group (PcG) is a diverse set of proteins required for maintenance of gene silencing during development. In a screen for conserved partners of the PcG protein Polycomblike (Pcl), we have identified a new protein, human CHMP1 (CHromatin Modifying Protein; CHarged Multivesicular body Protein), which is encoded by an alternative open reading frame in the PRSM1 gene and is conserved in both complex and simple eukaryotes. CHMP1 contains a predicted bipartite nuclear localization signal and distributes as distinct forms to the cytoplasm and the nuclear matrix in all cell lines tested. We have constructed a stable HEK293 cell line that inducibly overexpresses CHMP1 under ecdysone control. Overexpressed CHMP1 localizes to a punctate subnuclear pattern, encapsulating regions of nuclease-resistant, condensed chromatin. These novel structures are also frequently surrounded by increased histone H3 phosphorylation and acetylation. CHMP1 can recruit a PcG protein, BMI1, to these regions of condensed chromatin and can cooperate with co-expressed vertebrate Pcl in a Xenopus embryo PcG assay; this is consistent with a role in PcG function. In combination, these observations suggest that CHMP1 plays a role in stable gene silencing within the nucleus.

Effect of hyperinsulinemia on ovine fetal leucine kinetics during prolonged maternal fasting

1992 ◽  
Vol 263 (4) ◽  
pp. E696-E702 ◽  
Author(s):  
E. A. Liechty ◽  
D. W. Boyle ◽  
H. Moorehead ◽  
Y. M. Liu ◽  
S. C. Denne
Keyword(s):  
Protein Metabolism ◽  
Glucose Utilization ◽  
Whole Body ◽  
Postnatal Life ◽  
Body Protein ◽  
Leucine Oxidation ◽  
Leucine Kinetics ◽  
Ovine Fetus ◽  
Ovine Fetal

The primary effect of insulin on whole body protein metabolism in postnatal life is to reduce proteolysis. To assess the role of insulin in the regulation of protein metabolism in prenatal life, leucine kinetics were determined in the ovine fetus at baseline and in response to hyperinsulinemia. These measurements were made in each fetus in two different maternal states: ad libitum maternal feeding and after a 5-day maternal fast. Maternal fasting resulted in significant increases in baseline fetal leucine rate of appearance (Ra; 51.9 +/- 16.7 vs. 37.3 +/- 3.6 mumol/min, P < 0.05) and leucine oxidation (30.1 +/- 8.9 vs. 8.8 +/- 2.2 mumol/min, P < 0.05). Hyperinsulinemia, which was associated with significant increases in fetal glucose utilization, did not affect total fetal leucine R(a) or leucine release from fetal proteolysis in either maternal state. Under well-fed maternal conditions, hyperinsulinemia produced no changes in the fetal oxidative or nonoxidative disposal of leucine. In contrast, during maternal fasting, hyperinsulinemia reduced fetal leucine oxidation (11.0 +/- 3.7 vs. 31.1 +/- 8.9 mumol/min, P < 0.05) and increased the nonoxidative disposal of leucine (35.4 +/- 4.0 vs. 19.0 +/- 6.1 mumol/min, P < 0.05). This resulted in a change in the fetal leucine accretion rate from negative to positive (-20.9 +/- 7.5 vs. 7.5 +/- 6.7 mumol/min, P < 0.05). These results suggest that, under conditions of restricted maternal substrate intake, fetal hyperinsulinemia and the attendant increase in fetal glucose utilization are associated with increased protein synthesis rather than decreased protein breakdown, thereby improving fetal leucine carcass accretion.

Prognostic value and immunological role of PDCD1 gene in pan-cancer

2020 ◽  
Vol 89 ◽  
pp. 107080
Author(s):  
Yandong Miao ◽  
Jiangtao Wang ◽  
Qiutian Li ◽  
Wuxia Quan ◽  
Yingying Wang ◽  
...  
Keyword(s):  
Prognostic Value ◽  
Pan Cancer
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