scholarly journals Chitosan-coated poly(lactic-co-glycolic) acid nanoparticles as an efficient delivery system for Newcastle disease virus DNA vaccine

2014 ◽  
pp. 4609 ◽  
Author(s):  
Kai Zhao ◽  
Yang Zhang ◽  
Xiaohua Wang ◽  
Ci Shi ◽  
Xin Wang ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Dna Vaccine ◽  
Delivery System ◽  
Newcastle Disease ◽  
Glycolic Acid ◽  
Efficient Delivery

scholarly journals Development of PLGA-PEG-PLGA Hydrogel Delivery System for Enhanced Immunoreaction and Efficacy of Newcastle Disease Virus DNA Vaccine

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2505 ◽  
Author(s):  
Ying Gao ◽  
Hui Ji ◽  
Lin Peng ◽  
Xiuge Gao ◽  
Shanxiang Jiang
Keyword(s):  
Immune Responses ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Dna Vaccine ◽  
Delivery System ◽  
Newcastle Disease ◽  
Thermosensitive Hydrogel ◽  
Efficient Delivery ◽  
Dna Hydrogel ◽  
Highly Virulent

The highly contagious Newcastle disease virus (NDV) continues to threaten poultry all over the world. The NDV DNA vaccine is a promising solution to the current Newcastle disease (ND) challenges, and thus an efficient delivery system should be developed to facilitate the efficacy of DNA vaccines. In this study, we developed a DNA vaccine delivery system consisting of a triblock copolymer of poly(lactide co-glycolide acid) and polyethylene glycol (PLGA-PEG-PLGA) hydrogel in which the recombinant NDV hemagglutinin-neuraminidase (HN) plasmid was encapsulated. Its characteristics, security, immune responses, and efficacy against highly virulent NDV were detected. The results showed that the plasmids were gradually released in a sustained manner from the hydrogel, which improved the biological stability of the plasmids and demonstrated a high biocompatibility. The plasmids, when they were incorporated into the hydrogel delivery system, enhanced immune activation and provided 100% protection against the highly virulent NDV strain. Furthermore, we proved that this NDV DNA hydrogel vaccine could improve the lymphocyte proliferation and increase the immunological cytokine production via the PI3K/Akt pathway. These results indicate that the PLGA-PEG-PLGA thermosensitive hydrogel could be a promising delivery system for the NDV DNA vaccine in order to achieve a sustained supply of plasmids and induce potent immune responses.

Optimization of Preparation and Characterization of the Plasmid DNA from Newcastle Disease Virus Encapsulated in Chitosan Nanoparticles

2014 ◽  
Vol 1042 ◽  
pp. 19-25
Author(s):  
Ci Shi ◽  
Yan Wei Sun ◽  
Guang Yu Rong ◽  
Yang Zhang ◽  
Kai Zhao
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Dna Vaccine ◽  
Delivery System ◽  
Newcastle Disease ◽  
Chitosan Nanoparticles ◽  
Mucosal Vaccines ◽  
Physical And Chemical ◽  
Further Development

Newcastle disease (ND) is a highly contagious and lethality disease of poultry caused by Newcastle disease virus (NDV). ND is universally controlled by conventional vaccines. DNA vaccine is superior than conventional vaccines, but it also has some limitations. Nanopartciles mucosa delivery system using biodegradable materials could avoid defects of DNA vaccine. This study established a model with NDV DNA vaccine pVAX1-optiF immobilized into chitosan by complex coacervation method. Preparation process, physical and chemical characteristics of the nanoparticles were evaluated. The results demonstrated that pFDNA-CS-NPs showed suitable size, morphous regulation and well-distributed with a mean diameter of 199.5nm, polydispersity index of 0.336, encapsulation efficiency of 98.59±0.03%, loading capacity of 36.12±0.19 % and a Zeta potential of+11.2mV. This study is successfully preparated of NDV DNA vaccine mucosal immunity delivery system into chitosan as gene vector and laid a foundation for the further development of mucosal vaccines and drugs encapsulated in chitosan nanoparticles.

scholarly journals Intranasal Immunization With O-2′-Hydroxypropyl Trimethyl Ammonium Chloride Chitosan Nanoparticles Loaded With Newcastle Disease Virus DNA Vaccine Enhance Mucosal Immune Response in Chickens

2021 ◽  
Author(s):  
kai zhao ◽  
Beini Sun ◽  
Ci Shi ◽  
Yanwei Sun ◽  
Zheng Jin ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Ammonium Chloride ◽  
Dna Vaccine ◽  
Newcastle Disease ◽  
Plasmid Dna ◽  
Chitosan Nanoparticles ◽  
Burst Release ◽  
Antigen Delivery ◽  
Trimethyl Ammonium Chloride

Abstract Background: There is a great interest to develop strategies for enhancing antigen delivery to mucosal immune system as well as to identify mucosal active immunostimulating agents. To elevate the potential of O -2′-Hydroxypropyl trimethyl ammonium chloride chitosan (O-2′-HACC) nanoparticles as adjuvant and mucosal immune delivery carrier for DNA vaccine, we prepared the O-2′-HACC nanoparticles loaded with Newcastle disease virus F gene plasmid DNA with C3d6 molecular adjuvant (O-2′-HACC/pFDNA). Results: The O-2′-HACC/pFDNA had regular spherical morphology with a particle size of 202.3±0.52 nm, zeta potential of 50.8±8.21 mV, encapsulation efficiency of 90.74±1.10 %, and loading capacity of 49.84±1.20 %. The plasmid DNA could be sustainably released from the O-2′-HACC/pFDNA after an initial burst release. Intranasal vaccination of chickens immunized with O-2′-HACC/pFDNA not only induced higher anti-NDV IgG and sIgA antibody titers, but also significantly promoted lymphocyte proliferation and produced the higher levels of IL-2, IL-4, IFN-γ, CD4+ and CD8+ T lymphocytes than the NDV commercial attenuated live vaccine. Intranasal delivery of the O-2′-HACC/pFDNA enhanced humoral, cellular and mucosal immune responses, and protected chickens from the infection of highly virulent NDV than intramuscular delivery. Conclusions: This study indicated that the O-2′-HACC nanoparticles could be used as vaccine adjuvant and delivery system for mucosal immunity and have an immense application promise.

scholarly journals Intranasal immunization with O-2′-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles loaded with Newcastle disease virus DNA vaccine enhances mucosal immune response in chickens

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Kai Zhao ◽  
Beini Sun ◽  
Ci Shi ◽  
Yanwei Sun ◽  
Zheng Jin ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Ammonium Chloride ◽  
Dna Vaccine ◽  
Newcastle Disease ◽  
Plasmid Dna ◽  
Chitosan Nanoparticles ◽  
Burst Release ◽  
Antigen Delivery ◽  
Trimethyl Ammonium Chloride

Abstract Background There has been a great interest in developing strategies for enhancing antigen delivery to the mucosal immune system as well as identifying mucosal active immunostimulating agents. To elevate the potential of O-2ʹ-Hydroxypropyl trimethyl ammonium chloride chitosan (O-2ʹ-HACC) as an adjuvant and mucosal immune delivery carrier for DNA vaccine, we prepared the O-2ʹ-HACC loaded with Newcastle disease virus (NDV) F gene plasmid DNA and C3d6 molecular adjuvant (O-2ʹ-HACC/pFDNA microparticles). Results The O-2ʹ-HACC/pFDNA exhibited a regular spherical morphology with a particle size of 202.3 ± 0.52 nm, a zeta potential of 50.8 ± 8.21 mV, encapsulation efficiency of 90.74 ± 1.10%, and a loading capacity of 49.84 ± 1.20%. The plasmid DNA could be sustainably released from the O-2ʹ-HACC/pFDNA after an initial burst release. Intranasal vaccination of chickens immunized with O-2ʹ-HACC/pFDNA not only induced higher anti-NDV IgG and sIgA antibody titers but also significantly promoted lymphocyte proliferation and produced higher levels of IL-2, IL-4, IFN-γ, CD4+, and CD8 + T lymphocytes compared with the NDV commercial live attenuated vaccine. Intranasal delivery of the O-2ʹ-HACC/pFDNA enhanced humoral, cellular, and mucosal immune responses and protected chickens from the infection of highly virulent NDV compared with the intramuscular delivery. Conclusions Collectively, our findings indicated that the O-2ʹ-HACC could be used as a vaccine adjuvant and delivery system for mucosal immunity and have an immense application promise. Graphic Abstract

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