dna hydrogel
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2022 ◽  
Vol 23 ◽  
pp. 100680
Author(s):  
Qingyi Hu ◽  
Kejun Dong ◽  
Jie Ming ◽  
Wen Yang ◽  
Hongbo Wang ◽  
...  

2022 ◽  
Author(s):  
Sayantani Basu ◽  
Aishik Chakraborty ◽  
Abdul-Rahman Imad Alkiswani ◽  
Yasmeen Shamiya ◽  
Arghya Paul

Hydrogels, prepared from natural polymers, are attractive biomaterials for diverse biomedical applications due to their excellent biocompatibility and bioactivity. However, the majority of conventional hydrogels are mechanically weak and unsuitable...


Author(s):  
Xiujuan Liu ◽  
Meixiang Zhang ◽  
Ze Chen ◽  
Jiuqing Cui ◽  
Long Yang ◽  
...  

MicroRNA (miRNA) detection has attracted widespread interest as a tumor detection marker. In this work, a miRNA-responsive visual and temperature sensitive probe composed of a horseradish peroxidase (HRP)-encapsulated DNA hydrogel was designed and synthesized. The biosensor converted the miRNA hybridization signal to a photothermal effect which was measured using a digital thermometer. The substrate DNA linker strand of the hydrogel hybridizes with different sequences of miRNA resulting in the collapse of the hydrogel and the release of HRP. HRP oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) resulting in a color change and a strong photothermal effect was observed after shining near-infrared light on the oxidized product. The thermometer-based readout method has a wide linear range (0.5–4.0 µM) and a limit of detection limit of 7.8 nM which is comparable with traditional UV-vis absorption spectrometry detection and quantitative real time polymerase chain reaction methods. The low cost, ease of operation, and high sensitivity shows that this biosensor has potential for point-of-care biomolecular detection and biomedical applications.


2021 ◽  
Vol 16 (12) ◽  
pp. P12034
Author(s):  
S. Hu ◽  
Y. Jia

Abstract The solution-gate graphene field effect transistor (Sg-GFET), as a popular sensing platform, its applications are still hindered by the deficiency in all-solid-state, due to the dependence on liquid-state gate-dielectric. Inspired by DNA hydrogel which can provide microporous architecture to accommodate the fluidic analyte, moreover, its combination with graphene is believed to foster electron transport in the field of electrochemistry. We are interested to take advantage of DNA hydrogel's solid-state and capability for holding solution, and investigate whether it can replace the traditional solution. So pure DNA hydrogel, their complexes with GO (GO/DNA hydrogel) and RGO (RGO/DNA hydrogel) are studied herein. Their micro-porous 3D morphologies are demonstrated, their influences on the electrical characteristics of GFETs are carefully examined and proved to be able to maintain the typical bipolarity of Sg-GFET, firstly. Then, pure DNA hydrogel and GO/DNA hydrogel are selected as the optimized gate-dielectrics, because of their renewability after dehydration. Furthermore, by using aptamer-based heavy metal ions (Pb2+ and Hg2+) detections as proof-of-concept, the strategies for building the sensing platform based on the optimized hydrogel dielectric-gated GFETs are studied. It is found, for the purpose of substituting fluidic dielectric in traditional Sg-GFET, the scheme of directly mounting aptamer on graphene channel and coating pure DNA hydrogel on it is demonstrated to be better than the strategies of using GO/DNA hydrogel and hybriding aptamer probes in hydrogel scaffold. It is explained according to surface charge sensing mechanism. At last, the performances of the sensing platform based on the proposed DNA hydrogel gated GFETs are testified by the detections and selectivity examinations for Pb2+ and Hg2+. Conclusively, pure DNA hydrogel is expected to be a promising candidate in the future all-solid-state Sg-GFET.


2021 ◽  
Vol 7 (47) ◽  
Author(s):  
Ze Xiong ◽  
Sippanat Achavananthadith ◽  
Sophie Lian ◽  
Leigh Edward Madden ◽  
Zi Xin Ong ◽  
...  
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2021 ◽  
Author(s):  
Chi Yao ◽  
Rui Zhang ◽  
Jianpu Tang ◽  
Dayong Yang

2021 ◽  
Vol 188 (11) ◽  
Author(s):  
Liling Hao ◽  
XuTao Liu ◽  
Sunjie Xu ◽  
Faliang An ◽  
Huajie Gu ◽  
...  

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