Efecto in vitro del extracto hidroalcohólico de albahaca (Ocimum basilicum) sobre el crecimiento de Actinomyces viscosus (In vitro effect of the hydroalcoholic extract of basil (Ocimum basilicum) on the growth of Actinomyces viscosus)

El propósito de este estudio fue determinar el efecto in vitro del extracto hidroalcohólico de albahaca, con concentraciones al 5%, 10% y 15%, sobre Actinomyces viscosus, que es una de las bacterias que colonizan en primera instancia la superficie dental para dar lugar a la formación de la placa bacteriana, comparado estos extractos con un Gold standard conocido universalmente en la odontología para el control de la placa bacteriana como es la Clorhexidina al 0,12%. Teniendo como muestra 20 placas Petriconstituidas por agar sangre enriquecido con sangre de cordero al 5%, divididas en 5 grupos de estudio, (5 pozos expuestos al extracto al 5%, 5 pozos expuestos el extracto al 10%, 5 pozos expuestos al extracto al15%, 5 pozos expuestos a la Clorhexidina al 0,12% como grupo patrón y los últimos 5 pozos expuestos al agua destilada como grupo control; para la evaluación a las 96h, 120h, 144hy 168h). El efecto in vitro del extracto de albahaca se determinó midiendo los halos de inhibición de crecimiento bacteriano formado en la superficie de las 20 placas Petri. Las mediciones se realizaron del cuarto al séptimo día de incubación a 37°C. Se determinó que el efecto in vitro del extracto hidroalcohólico de albahaca al 10% y al 15% esaltamente significativo frente al Actinomyces viscosus expuesto a partir de las 120h, aumentando éste hasta las 168h. Así mismo se determinó que a mayor concentración del extracto, mayor efecto antimicrobiano.La metodología usada permite confirmar la efectividad del extracto hidroalcohólico de albahaca con diferentes concentraciones frente al Actinomyces viscosus.

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In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants

International Journal of Horticultural Science ◽

10.31421/ijhs/25/3-4/3930 ◽

2019 ◽

Vol 25 (3-4) ◽

Author(s):

E. Enkhbileg ◽

M. G. Fári ◽

E. Kurucz

Keyword(s):

Root Growth ◽

Plant Pathogens ◽

Shoot Growth ◽

Ocimum Basilicum ◽

Shoot Induction ◽

Vitro Effect ◽

Secondary Shoot ◽

Detailed Protocol ◽

Culinary Herb

Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration.

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The In Vitro Effect of Hydroalcoholic Extract of Artemisia absinthium on the Growth of Leishmania major (MRHO/IR/75/ER) in Peritoneal Macrophages from BALB/c Mice

Jundishapur Journal of Microbiology ◽

10.5812/jjm.77302 ◽

2018 ◽

Vol In Press (In Press) ◽

Author(s):

Ali Rahiminejad ◽

Khosrow Hazrati Tappeh ◽

Shahram Seyyedi ◽

Peyman Mikaili

Keyword(s):

Leishmania Major ◽

Peritoneal Macrophages ◽

Artemisia Absinthium ◽

Hydroalcoholic Extract ◽

Vitro Effect

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In vitro effect of extract of garlic's wood (Gallesia integrifolia Sprengel) Harms on engorged ticks and larvae of Ripicephalus Boophilus microplus

Scientific Electronic Archives ◽

10.36560/1152018679 ◽

2018 ◽

Vol 11 (5) ◽

pp. 99-103

Author(s):

A. S. Dias ◽

A. M. Tanure ◽

R. M. Bertonceli

Keyword(s):

Herbal Medicine ◽

Tick Control ◽

Boophilus Microplus ◽

Cattle Tick ◽

Alcoholic Extract ◽

Hydroalcoholic Extract ◽

Tropical Area ◽

Vitro Effect ◽

Production Losses

The cattle tick Rhipicephalus Boophilus microplus is responsible for high losses in cattle stocking in tropical area, the losses related to parasitism of this agent represents up to 80% of production losses, represented by spoliation, damage to the leather, hematozoa transmission, among others. The problem of increased of resistance to chemical ectoparasiticids has led to the search for new alternatives, among them there is the herbal medicine, in that it is more accessible, less polluting, suspected delay the resistance phenomenon. In this study, it sought to evaluate the efficacy of alcoholic extract of Gallesia integrifolia (garlic's wood) on tick control R. B. microplus. The test was carried out on engorged ticks and on larvae in impregnated papers in vitro. It was found that the hydroalcoholic extract tested at concentrations of 100, 50, 25, 10, 5, 2.5 and 1% was effective on mortality of adult ticks on 100% concentration from about 5% and on larvae, in 10% concentrations. And at 2.5%, the action on the mortality of larvae was considered satisfactory by the official regulatory criteria. These in vitro results are presented favorable regarding the candidacy of G. integrifolia extract for control of R. B. microplus in cattle.

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In vitro effect of hydroalcoholic extract of Peganum harmala on human ureteric contractions

Journal of Renal Injury Prevention ◽

10.15171/jrip.2019.44 ◽

2019 ◽

Vol 8 (3) ◽

pp. 235-239

Author(s):

Majid Shirani ◽

Zahra Lorigooini ◽

Arsham Pouriamofrad ◽

Zahra Keivani Hafshejani

Keyword(s):

Ureteral Obstruction ◽

Nacl Solution ◽

Peganum Harmala ◽

Tissue Samples ◽

Hydroalcoholic Extract ◽

Significant Difference ◽

In Vitro Effects ◽

Vitro Effect ◽

Human Ureter

Introduction: Ureteral obstruction by stones is one of the most common urological problems. Objectives: This study aimed to investigate the in vitro effects of hydroalcoholic extract of Peganum harmala L. on the contraction of the human ureter. Materials and Methods: In this study, 28 samples of human ureter tissue were studied. Six tissue samples were examined for the evaluation of P. harmala extract at concentrations of 1, 2, and 4 mg/mL. Moreover, five tissue samples were examined for checking NaCl solution since, six pieces of tissue for the examination of prazosin 10-8M solution. Results: There was a significant difference between the second contraction of P. harmala group with concentration of 4 mg/mL and NaCl group (P<0.001). Additionally, a significant difference between the prazosin and P. harmala groups with a concentration of 4 mg/mL (P=0.048) was observed. There is a marginal difference between the secondary contractions in two groups. A significant decrease in secondary contraction (15 minutes after adding the solution) was observed between the prazosin group and the P. harmala group at a concentration of 1 mg/mL (P<0.001). Moreover, the secondary contraction of the prazosin group showed a significant decrease compared with the NaCl group (P<0.001). Conclusion: Administration of P. harmala extract can be useful in reducing urinary human ureteric contractions while the best effect was achieved at 4 mg/mL of P. harmala extract.

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