In vitro effect of different cytokinin types (BAP, TDZ) on two different Ocimum basilicum cultivars explants

Ocimum basilicum L. (sweet basil) is an economically and ethnobotanically important aromatic, medicinal, ornamental and culinary herb, with a very wide gene pool, that is sensitive to cold and prone to several plant pathogens that can demolish harvest and lessen yield. In this research, the effects of BAP (6-Benzylaminopurine) and TDZ (Thidiazuron) on different genotypes for in vitro cloning were determined, in order to provide a detailed protocol guide concerning Ocimum basilicum L. propagation. The results from the O. basilicum seed propagations revealed that the best condition for the secondary shoot growth is with 5.0 mg/l TDZ or 1.5 mg/l BAP on all types of explants except the root, the secondary root growth can be obtained on all types explant with any BAP concentration and all cytokinins can induce callus on all types of explants. On the whole, it shows that multiple secondary shoot induction and regeneration in Ocimum basilicum L. is regulated by appropriate cytokinin concentration.

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Antagonism of Plant Pathogens by Calotropis procera

Journal of Agricultural Science ◽

10.5539/jas.v13n4p120 ◽

2021 ◽

Vol 13 (4) ◽

pp. 120

Author(s):

Karla Danielle Nogueira Cardoso ◽

Maria Josiane Martins ◽

Thaisa Aparecida Neres de Souza ◽

Isabelle Carolyne Cardoso ◽

Patrícia Nirlane da Costa Souza ◽

...

Keyword(s):

Growth Rate ◽

Aqueous Extract ◽

Mycelial Growth ◽

Plant Pathogens ◽

Calotropis Procera ◽

Mycelial Growth Rate ◽

Vitro Effect ◽

Fungal Colony

Phomopsis sojae and Sclerotinia sclerotiorum are responsible for stem and pod dryness and white mold in soybean. These pathologies directly affect the quality of seeds/grains and compromise the entire plant. The use of extracts from different plants has been the subject of research for the control of several phytopathogens. Calotropis procera is among botanical species that synthesize efficient compounds for biocontrol. In this context, the aim of this study was to evaluate the in vitro effect of C. procera aqueous extract on P. sojae and S. sclerotiorum. The experiment was carried out in completely randomized blocks in a 2 × 5 factorial scheme (two fungi and five extract concentrations 0%, 5%, 10%, 15% and 20%) with 4 replicates. C. procera aqueous extract concentrations were added to Petri dishes containing PDA. After 48 hours, the mycelial growth rate was evaluated. After seven days of incubation, the fungal colony area, sporulation, and germination of P. sojae and S. sclerotiorum were evaluated. There was significant interaction between fungi × extract concentrations (p < 0.05) for all variables analyzed. The mycelial growth rate of P. sojae was lower than that of S. sclerotiorum. The diameter of the P. sojae fungal colony was smaller than that of S. sclerotiorum when concentrations of 5%, 10% and 15% were used. As the extract concentration increased, fungi sporulation and germination reduced.

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Effects of salinity on in vitro growth and photosynthesis of common centaury (Centaurium erythraea Rafn.)

Archives of Biological Sciences ◽

10.2298/abs0702129s ◽

2007 ◽

Vol 59 (2) ◽

pp. 129-134 ◽

Cited By ~ 16

Author(s):

B. Siler ◽

Danijela Misic ◽

Biljana Filipovic ◽

Zorica Popovic ◽

Tijana Cvetic ◽

...

Keyword(s):

Root Growth ◽

Chlorophyll Content ◽

Shoot Growth ◽

In Vitro Growth ◽

Total Chlorophyll ◽

Adventitious Buds ◽

Nacl Concentration ◽

Chlorophyll A And B ◽

Centaurium Erythraea

The effects of salinity on in vitro growth, morphogenesis, photosynthetic rate (Pn), PSII efficiency (Fv/Fm), and chlorophyll content were investigated in Centaurium erythraea Rafn. Root growth was more adversely affected by increasing NaCl concentration than was shoot growth. High salt concentrations were effective in induction of axillary and adventitious buds on shoots, with 400 mM NaCl being the most efficient one. Values of Pn and Fv/Fm increased at moderate salt levels, but decreased when 400 mM NaCl was applied. Chlorophyll a and b contents and total chlorophyll had a decreasing trend with increasing supply of NaCl in the growth medium. .

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Efecto in vitro del extracto hidroalcohólico de albahaca (Ocimum basilicum) sobre el crecimiento de Actinomyces viscosus (In vitro effect of the hydroalcoholic extract of basil (Ocimum basilicum) on the growth of Actinomyces viscosus)

Ciencia y Desarrollo ◽

10.21503/cyd.v20i1.1409 ◽

2017 ◽

Vol 20 (1) ◽

pp. 65

Author(s):

Herbert Cosio ◽

Hilda Rodríguez

Keyword(s):

Gold Standard ◽

Ocimum Basilicum ◽

Hydroalcoholic Extract ◽

Vitro Effect

El propósito de este estudio fue determinar el efecto in vitro del extracto hidroalcohólico de albahaca, con concentraciones al 5%, 10% y 15%, sobre Actinomyces viscosus, que es una de las bacterias que colonizan en primera instancia la superficie dental para dar lugar a la formación de la placa bacteriana, comparado estos extractos con un Gold standard conocido universalmente en la odontología para el control de la placa bacteriana como es la Clorhexidina al 0,12%. Teniendo como muestra 20 placas Petriconstituidas por agar sangre enriquecido con sangre de cordero al 5%, divididas en 5 grupos de estudio, (5 pozos expuestos al extracto al 5%, 5 pozos expuestos el extracto al 10%, 5 pozos expuestos al extracto al15%, 5 pozos expuestos a la Clorhexidina al 0,12% como grupo patrón y los últimos 5 pozos expuestos al agua destilada como grupo control; para la evaluación a las 96h, 120h, 144hy 168h). El efecto in vitro del extracto de albahaca se determinó midiendo los halos de inhibición de crecimiento bacteriano formado en la superficie de las 20 placas Petri. Las mediciones se realizaron del cuarto al séptimo día de incubación a 37°C. Se determinó que el efecto in vitro del extracto hidroalcohólico de albahaca al 10% y al 15% esaltamente significativo frente al Actinomyces viscosus expuesto a partir de las 120h, aumentando éste hasta las 168h. Así mismo se determinó que a mayor concentración del extracto, mayor efecto antimicrobiano.La metodología usada permite confirmar la efectividad del extracto hidroalcohólico de albahaca con diferentes concentraciones frente al Actinomyces viscosus.

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Ethephon and secondary shoot induction in Gentian (Gentiana spp.) hybrids in vitro

Scientia Horticulturae ◽

10.1016/j.scienta.2014.09.020 ◽

2014 ◽

Vol 179 ◽

pp. 170-173 ◽

Cited By ~ 3

Author(s):

Marzieh Keshavarzi ◽

Keith A. Funnell ◽

Julian A. Heyes ◽

David J. Woolley

Keyword(s):

Shoot Induction ◽

Secondary Shoot

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Physical Environment in vitro Affects Laboratory and Nursery Growth of Micropropagated Hostas

HortTechnology ◽

10.21273/horttech.10.4.754 ◽

2000 ◽

Vol 10 (4) ◽

pp. 754-757 ◽

Cited By ~ 6

Author(s):

J. Adelberg ◽

M. Kroggel ◽

J. Toler

Keyword(s):

Root Growth ◽

Liquid Culture ◽

Shoot Growth ◽

Dry Weight ◽

Ex Vitro ◽

Agar Culture ◽

Ex Vitro Growth ◽

Weight Analysis ◽

Outdoor Nursery

Hosta ×hybrid Tratt. `Blue Cadet' and Hosta tokudama Tratt. `Newberry Gold' were micropropagated in shaken liquid culture and on agar media, in conventional vessels and vessels modified for ventilation in vitro. Acclimatization under intermittent mist and growth in an outdoor nursery during the late spring and summer were monitored by dry weight analysis of sample plants every 4 days for a 60-day period (ex vitro growth). Results for `Newberry Gold' were 1) in vitro shoot growth was greater in liquid than agar culture, regardless of vessel; 2) shoots from agar or liquid culture grew at similar rates ex vitro; 3) ex vitro root growth was greater for liquid than agar cultured plants, regardless of vessel type. Results for `Blue Cadet' were 1) in vitro and ex vitro shoot growth was greater in liquid than agar culture regardless of vessel type and 2) ex vitro root growth was greatest for liquid cultured plants from conventional vessels. Ventilated vessels were generally beneficial for agar but not liquid culture. Benefits of liquid culture for micropropagation of Hosta found in vitro are at least maintained and sometimes enhanced during ex vitro growth in the mist bed and nursery.

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