Control of the genetic purity of sunflower lines and hybrids by an isozyme analysis

2017 ◽  
Vol 1 (66) ◽  
pp. 82-86
Author(s):  
Saida Guchetl ◽  
◽  
Tatyana Tchelustnikova ◽  
Tatyana Antonova ◽  
Alena Umanetz ◽  
...  
Keyword(s):  
Isozyme Analysis ◽  
Genetic Purity

scholarly journals Differentiation and genetic variability in natural populations of Anopheles (N.) triannulatus (Neiva& Pinto, 1922) of Brazilian Amazonia

2004 ◽  
Vol 64 (2) ◽  
pp. 327-336 ◽  
Author(s):  
J. M. M. Santos ◽  
J. F. Maia ◽  
W. P. Tadei
Keyword(s):  
Genetic Variability ◽  
Natural Populations ◽  
Isozyme Analysis ◽  
Brazilian Amazonia ◽  
F Statistics

Populations of Anopheles triannulatus from Macapá (AP), Aripuanã (MT), Ji-Paraná (RO), and Manaus-Janauari Lake (AM) were studied using 16 enzymatic loci. The results of the isozyme analysis showed that the population of Macapá presented higher polymorphism (56.3%). The lowest variability was observed in the population of Manaus (p = 25.0; Ho = 0.077 ± 0.046). The results of Wright's F statistics showed unbalance due to excess of homozygotes (Fis > Fst), denoting a certain intrapopulational differentiation. Although the populations are genetically very close (D = 0.003 - 0.052), the dendrogram separates the populations in two groups: Macapá separated from that of Manaus, Ji-Paraná, and Aripuanã. This result may suggest a reduction in the genic flow, which possibly had some influence in the substructuration of the populations.

A modified DNA extraction protocol and its utility in seed genetic purity assessment

2011 ◽  
Vol 39 (1) ◽  
pp. 236-242 ◽  
Author(s):  
S.N. Sharma ◽  
V. Kumar ◽  
G. Singh ◽  
R. Sharma
Keyword(s):  
Dna Extraction ◽  
Genetic Purity ◽  
Purity Assessment ◽  
Extraction Protocol ◽  
Modified Dna

Taxonomy of Penicillium chrysogenum and related xerophilic species, based on isozyme analysis

1997 ◽  
Vol 101 (5) ◽  
pp. 617-624 ◽  
Author(s):  
Søren Banke ◽  
Jens C. Frisvad ◽  
Søren Rosendahl
Keyword(s):  
Penicillium Chrysogenum ◽  
Isozyme Analysis

Genetic purity analysis of cotton (Gossypium spp.) hybrids using SSR markers

2010 ◽  
Vol 38 (2) ◽  
pp. 358-366 ◽  
Author(s):  
P. Selvakumar ◽  
R. Ravikesavan ◽  
A. Gopikrishnan ◽  
K. Thiyagu ◽  
S. Preetha ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Purity ◽  
Gossypium Spp ◽  
Purity Analysis

scholarly journals Use of molecular marker techniques in seed testing by Brazilian seed companies

1998 ◽  
Vol 55 (spe) ◽  
pp. 79-82 ◽  
Author(s):  
P.T. Della Vecchia ◽  
C.A.R. da Silva ◽  
P. Terenciano-Sobrinho
Keyword(s):  
Molecular Marker ◽  
New Technologies ◽  
Seed Quality ◽  
Genetic Purity ◽  
Process Step ◽  
Seed Testing ◽  
Routine Basis ◽  
Seed Market ◽  
Key Issues ◽  
High Quality Seed

Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD, microsatelites, AFLP) for genetic purity determinations in seed testing. As far as we know, no Brazilian seed company is using, on regular basis, RAPD or other molecular marker techniques in the determination of genetic purity in seed testing. Most of these are using morphological or physiological traits expressed by seed, seedling or mature plant and/or electrophoresis of seed or seedling proteins/isoenzymes for that purpose. Main reasons for that are: DNA molecular marker techniques are relatively new; lack of specialized personnel to run DNA molecular marker assays on routine basis; higher cost/sample when compared to proteins/isoenzymes electrophoresis.

EFFECTS OF ISOLATION DISTANCE ON CONTAMINATION IN SWEETCLOVER

1972 ◽  
Vol 52 (4) ◽  
pp. 517-524 ◽  
Author(s):  
B. P. GOPLEN ◽  
D. A. COOKE ◽  
P. PANKIW
Keyword(s):  
Honey Bees ◽  
Genetic Purity ◽  
Isolation Distance ◽  
Isolation Distances ◽  
Contamination Levels ◽  
High Level

The recessive low coumarin gene cu was used as a marker to study the effects of isolation distance on contamination levels in sweetclover pollinated by honey bees. A 46-m isolation distance was found inadequate to maintain a high level of genetic purity. Considerable contamination from crossing resulted with isolation distances from 46 to 804 m when there was little competitive bloom from other entomophilous crops. A highly attractive and competitive crop of rapeseed appeared to serve as a very effective isolation barrier to minimize contamination. A higher amount of contamination occurred in the borders of the low-coumarin isolation plots of one test. The results of this study are sufficient to question the existing isolation standards for sweetclover, but are not adequate to formulate new standards.

scholarly journals Pengujian Kemurnian Genetik Benih Padi Galur F3 (Pandanwangi x PTB33) Terseleksi Menggunakan Marka Molekuler Simple Sequence Repeats (SSR)

Agrikultura ◽  
2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Syindy Raffini Nasihin ◽  
Wieny H. Rizky ◽  
Nono Carsono
Keyword(s):  
Simple Sequence Repeats ◽  
Simple Sequence Repeat ◽  
Brown Planthopper ◽  
Plant Analysis ◽  
Current Experiment ◽  
Genetic Purity ◽  
Experimental Station ◽  
Purity Testing ◽  
Brown Planthopper Resistance ◽  
Simple Sequence

ABSTRACTSeed Purity Testing of F3 Progeny of Rice Lines Derived from a Cross between Pandanwangi x PTB-33 Estimated by Simple Sequence Repeat MarkersSeeds with high purity is required to produce maximum crop yield. Genetic purity of selected F3rice seed progenies derived from a cross between Pandanwangi x PTB33 was estimated by SSR(simple sequence repeats) molecular markers. Objective of current experiment was to obtain riceseed with high genetic purity (100%) in terms of homogeneity of alleles. The experiment wasconducted at Laboratory of Plant Analysis and Biotechnology, meanwhile field experiment wasperformed at Ciparanje Experimental Station, Faculty of Agriculture, Universitas Padjadjaran.Based on primer designed by Bradbury (aromatic trait) and primers RM589 and RM586 (supposedto correlate with brown planthopper resistance gene), seeds of F3 selected had 100% geneticpurity. SSR markers applied for each offspring were able to demonstrate the purity of the seed.Genotypes with 100% genetic purity can be continued for propagating their seeds.Keywords: F3, seed purity, seed rice, SSR markersABSTRAKBenih dengan kemurnian genetik tinggi sangat diperlukan untuk produksi tanaman yangmaksimal. Kemurnian genetik padi generasi F3 hasil persilangan Pandanwangi x PTB-33 diestimasidengan menggunakan marka molekuler SSR. Percobaan ini bertujuan untuk mendapatkan generasiF3 yang memiliki kemurnian genetik 100%. Penelitian dilaksanakan di Kebun PercobaanCiparanje, Fakultas Pertanian Universitas Padjadjaran dan Laboratorium Analisis dan BioteknologiTanaman. Hasil analisis menggunakan primer Bradbury menunjukkan 100% benih murniberdasarkan karakter aromatik, begitupun berdasarkan karakter ketahanan terhadap wereng(primer RM589 dan RM586) menunjukkan 100% benih murni. Marka molekuler SSR yangdigunakan untuk verifikasi kemurnian mampu menunjukkan kemurnian genetik benih padi yangtinggi. Genotip PP dapat dilanjutkan untuk pengujian dan atau perbanyakan benih sumber.Kata kunci: benih padi, F3, marka SSR, uji kemurnian genetik

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