Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence

In insect-pollinated crops, gene flow is affected by numerous factors including crop characteristics, mating system, life history, pollinators, and planting management practices. Previous studies have concentrated on the impact of distance between genetically engineered (GE) and conventional fields on adventitious presence (AP) which represents the unwanted presence of a GE gene. Variables other than distance, however, may affect AP. In addition, some AP is often present in the parent seed lots used to establish conventional fields. To identify variables that influence the proportion of AP in conventional alfalfa fields, we performed variable selection regression analyses. Analyses based on a sample-level and a field-level analysis gave similar, though not identical results. For the sample-level model, distance from the GE field explained 66% of the variance in AP, confirming its importance in affecting AP. The area of GE fields within the pollinator foraging range explained an additional 30% of the variation in AP in the model. The density of alfalfa leafcutting bee domiciles influenced AP in both models. To minimize AP in conventional alfalfa seed fields, management practices should focus on optimizing isolation distances while also considering the size of the GE pollen pool within the pollinator foraging range, and the foraging behavior of pollinators.

Effective isolation distance for prevention of cassava virus infections in Uganda

Cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) are the major viral diseases of cassava in Uganda. Although isolation distance of “50 m” has been recommended by MAAIF in Uganda for prevention of virus infections in crops, the minimum isolation distance has not been verified for effectiveness in cassava. This study assessed the effective isolation distance for management of viral diseases in cassava. Virus-clean cassava cultivars (NASE 03, NASE 14 and NAROCASS 1) from farmers’ fields were used as field sourced (FS) planting materials. Tissue culture (TC) material of the same cultivars were sourced from the National Crops Resources Research Institute and Makerere University Agricultural Research Institute tissue culture laboratories. Both FS and TC materials were tested at isolation distances of 50, 100, 150 and 250 m for virus prevention. The experiment was laid out in a randomised complete block design and was run for 12 months after planting (MAP). Mean CBSD/CMD prevalence significantly varied (P<0.05) among isolation distances in both FS and TC plants, and the 250 m isolation distance was the most effective in reducing disease prevalence. Across cultivars and planting material category at 12 MAP, the 50 m isolation distance had the highest foliar incidence for CBSD (29.2%) and CMD (16.1%); while severity for CBSD was 1.4 and 1.2 for CMD. At 250 m, all FS and TC plants had CBSD/CMD severity of 1.0 and 0% incidence. These results show that 250 m isolation distance can provide an option to disseminate popular, but CBSD/CMD susceptible cassava cultivars thereby manage CBSD/CMD.

Isolation distance between municipal solid waste landfills and drinking water wells for bacteria attenuation and safe drinking

Groundwater pollution and human health risks caused by leachate leakage have become a worldwide environmental problem, and the harm and influence of bacteria in leachate have received increased attention. Setting the isolation distance between landfill sites and groundwater isolation targets is particularly important. Firstly, the intensity model of pollutant leakage source and solute transport model were established for the isolation of pathogenic Escherichia coli. Then, the migration, removal and reduction of bacteria in the aerated zone and ground were simulated. Finally, the isolation distance was calculated based on the acceptable water quality limits, and the influence of hydrogeological arameters was analyzed based on the parameter uncertainty. The results of this study suggest that the isolation distances vary widely ranging from 106 m–5.46 km in sand aquifers, 292 m–13.5 km in gravel aquifers and 2.4–58.7 km in coarse gravel aquifers. The gradient change of groundwater from 0.001 to 0.05 resulted in the isolation distance at the highest gradient position being 2–30 times greater than that at the lowest gradient position. There was a difference in the influence of the thickness of the vadose zone. For example, under the same conditions, with the increase of the thickness of the aeration zone, the isolation distance will be reduced by 1.5–5 times, or under the same thickness of the aeration zone, the isolation distance will be significantly shortened. Accordingly, this needs to be determined based on specific safety isolation requirements. In conclusion, this research has important guiding significance for the environmental safety assessment technology of municipal solid waste landfill.

Cytoplasmic male sterility as a biological confinement tool for maize coexistence: optimization of pollinator spatial arrangement

Cytoplasmic male sterility (CMS) allows efficient biological confinement of transgenes if pollen-mediated gene flow has to be reduced or eliminated. For introduction of CMS maize in agricultural practice, sufficient yields comparable with conventional systems should be achieved. The plus-cultivar-system in maize offers a possibility for biological confinement together with high and stable yields whereas pollinator amount and distribution within the CMS crop is crucial. The aim of this EU-funded study was to identify the best proportion (10, 15, and 20%) and spatial arrangement (inserted rows, mixed seeds) of the pollinator within the CMS maize cultivar under field conditions in the Czech Republic, in Germany and in Spain. In Germany and in the Czech Republic, a pollinator proportion of 10% produced significantly lower yield than the treatments with a pollinator proportion of 15% and 20%. Differences in yield between row and mix arrangements were not detected. No differences between the tested arrangements occurred in Spain. With respect to practical conditions, a pollinator proportion of 15% can be recommended for achieving a satisfactory yield. CMS maize cultivar released no or merely a small amount of pollen and self-pollinated plants developed no or only a small number of kernels indicating that currently recommended isolation distances between genetically modified (GM) and non-GM fields can be substantially shortened if the CMS confinement tool is used.

A Lévy-flight diffusion model to predict transgenic pollen dispersal

The containment of genetically modified (GM) pollen is an issue of significant concern for many countries. For crops that are bee-pollinated, model predictions of outcrossing rates depend on the movement hypothesis used for the pollinators. Previous work studying pollen spread by honeybees, the most important pollinator worldwide, was based on the assumption that honeybee movement can be well approximated by Brownian motion. A number of recent studies, however, suggest that pollinating insects such as bees perform Lévy flights in their search for food. Such flight patterns yield much larger rates of spread, and so the Brownian motion assumption might significantly underestimate the risk associated with GM pollen outcrossing in conventional crops. In this work, we propose a mechanistic model for pollen dispersal in which the bees perform truncated Lévy flights. This assumption leads to a fractional-order diffusion model for pollen that can be tuned to model motion ranging from pure Brownian to pure Lévy. We parametrize our new model by taking the same pollen dispersal dataset used in Brownian motion modelling studies. By numerically solving the model equations, we show that the isolation distances required to keep outcrossing levels below a certain threshold are substantially increased by comparison with the original predictions, suggesting that isolation distances may need to be much larger than originally thought.