scholarly journals Investigation of E2, E5, and E6 gene expression and DNA in situ fragmentation findings associated with progressive and regressive changes in benign neoplastic cutaneous lesions arising naturally from bovine papillomavirus-1 infection

2016 ◽  
Vol 72 (9) ◽  
pp. 549-557 ◽  
Author(s):  
Mehmet Eray Alcigir ◽  
Sevil Atalay Vural ◽  
Mehmet Ozkan Timurkan
Keyword(s):  
Gene Expression ◽  
Bovine Papillomavirus ◽  
Chain Reaction ◽  
Cutaneous Lesions ◽  
E6 Gene ◽  
Polymerase Chain ◽  
Hematoxylin Eosin ◽  
Regressive Phase ◽  
Viral Entity

This study investigated the progressive and regressive phases of bovine papillomavirus (BPV)-1-induced papilloma and fibropapilloma in terms of the distribution of viral antigenicity; E2, E5, and E6 gene activity and expression; and apoptosis (evaluated using DNA in situ fragmentation). All samples from 74 bovine cases of cutaneous lesions with suspected neoplastic changes collected from different skin areas were evaluated. The lesions were diagnosed as papilloma or fibropapilloma using routine hematoxylin–eosin and Mallory’s trichrome staining protocols. Feulgen reactions suggested a possible viral entity, and BPV-1 antigenicity was immunohistochemically observed in 22 cases. E gene expression in neoplastic tissues was evaluated using chromogenic in situ hybridization and reverse-transcription polymerase chain reaction, and the TUNEL method was used to evaluate DNA in situ fragmentation. Differences between progressive and regressive phases in these lesions were analyzed. Ultimately, the number of cases in the progressive phase (18) and regressive phase (4) were determined. Although E2 and E5 gene expression was observed in both phases, E6 gene distribution differed from the other E genes distribution in the progressive phase. E5 gene expression appeared stronger in oncogenic fibrocytes and fibroblasts, whereas E2 and E6 gene expression was weaker in epidermal cells and cells in the regressive phase. Apoptosis, considered the best indicator of neoplastic regression, was found to be associated with the regressive phase but was observed insufficiently in epidermal and dermal samples of the progressive-phase cases. In conclusion, this work provides significant insights on the molecular and immunopathological features of BPV-1.

scholarly journals Detection of MBL-2 gene expression in intestinal biopsies of celiac patients by in situ reverse transcription polymerase chain reaction

10.4081/825 ◽  
2009 ◽  
Vol 47 (2) ◽  
pp. 177 ◽  
Author(s):  
M Boniotto ◽  
O Radillo ◽  
L Braida ◽  
D Pirulli ◽  
A Città
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals The contribution of increased collagen synthesis to human glomerulosclerosis: a quantitative analysis of alpha 2IV collagen mRNA expression by competitive polymerase chain reaction.

1992 ◽  
Vol 176 (6) ◽  
pp. 1571-1576 ◽  
Author(s):  
E P Peten ◽  
L J Striker ◽  
M A Carome ◽  
S J Elliott ◽  
C W Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Collagen Synthesis ◽  
Type Iv Collagen ◽  
Chain Reaction ◽  
Collagen Mrna ◽  
Type Iv ◽  
Polymerase Chain

We previously reported that one of the main components of the sclerotic material in human glomerular diseases was type IV collagen. In this study we examined the contribution of increased synthesis to this process at the gene expression level. Sufficient material has not been available to study type IV collagen synthesis by normal or sclerotic glomeruli in humans. We took advantage of the availability of nephrectomy specimens from patients with renal carcinoma, and of the observation that approximately 50% of these patients develop varying degrees of glomerulosclerosis. We microdissected glomeruli from 10 patients and analyzed them using in situ reverse transcription coupled with polymerase chain reaction (PCR) analyses (in situ RT-PCR). alpha 2IV collagen mRNA, after reverse transcription into cDNA, was detected in all patients and appeared to be increased in those with glomerulosclerosis (n = 5). A competitive PCR assay was developed to quantitate this change. There was an average 3.7-fold increase in glomerular type IV collagen cDNA in patients with significant sclerosis. This change was not due to an increased number of glomerular cells. Thus, glomerulosclerosis in humans is associated with an elevation of glomerular type IV collagen gene expression, suggesting that increased synthesis of type IV collagen may represent one component of this process.

scholarly journals First report of bovine papillomavirus genotypes in cutaneous lesions by polymerase chain reaction (PCR) and direct sequencing in Panama

2019 ◽  
Vol 18 (32) ◽  
pp. 1098-1104
Author(s):  
González Rita ◽  
Murillo Manuel ◽  
Elena Castillo M. Hilda ◽  
Jaén Marcelino ◽  
Villalobos-Cortés Axel
Keyword(s):  
Polymerase Chain Reaction ◽  
Direct Sequencing ◽  
Bovine Papillomavirus ◽  
Chain Reaction ◽  
Cutaneous Lesions ◽  
First Report ◽  
Polymerase Chain

Localization ofApaf1 gene expression in the early development of the mouse by means of in situ reverse transcriptase-polymerase chain reaction

2005 ◽  
Vol 234 (1) ◽  
pp. 215-221 ◽  
Author(s):  
Matthias Müller ◽  
Joachim Berger ◽  
Nikolaus Gersdorff ◽  
Francesco Cecconi ◽  
Rainer Herken ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Early Development ◽  
Chain Reaction ◽  
Polymerase Chain

Cytokine Gene Expression: Analysis using Northern Blotting, Polymerase Chain Reaction and in situ Hybridization

1991 ◽  
Vol 119 (1) ◽  
pp. 163-179 ◽  
Author(s):  
Margaret J. Dallman ◽  
Robert A. Montgomery ◽  
Christian P. Larsen ◽  
Alkwin Wanders ◽  
Alvin F. Wells
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Gene Expression Analysis ◽  
Northern Blotting ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Chain Reaction ◽  
Polymerase Chain

Molecular Diagnosis of Sarcomas: Chromosomal Translocations in Sarcomas

2006 ◽  
Vol 130 (8) ◽  
pp. 1199-1207 ◽  
Author(s):  
Alexander Lazar ◽  
Lynne V. Abruzzo ◽  
Raphael E. Pollock ◽  
Sangkyou Lee ◽  
Bogdan Czerniak
Keyword(s):  
Scientific Literature ◽  
Diagnostic Methods ◽  
Chromosomal Translocations ◽  
Molecular Testing ◽  
Fusion Genes ◽  
Chain Reaction ◽  
Extensive Experience ◽  
Polymerase Chain ◽  
Hematoxylin Eosin

Abstract Context.—Sarcomas are rare, numerous in type, and often difficult to definitively classify. Work in the last 2 decades has revealed that a significant subset of sarcomas are associated with specific chromosomal translocations producing chimeric (fusion) genes that play a role in the sarcomas' biology and are helpful in their differential diagnosis. Objective.—To briefly review the sarcomas associated with specific translocations presenting Ewing sarcoma and synovial sarcoma as archetypes and to further explain how cytogenetic and molecular biologic approaches are being used in the diagnosis of sarcomas. Data Sources.—This work is based on a selected review of the relevant medical and scientific literature and our extensive experience with molecular testing in sarcomas. Conclusions.—In addition to, and complementing, the traditional diagnostic methods of examination of hematoxylin-eosin stained slides, immunohistochemistry, and sound clinical-pathologic correlation, additional cytogenetic and molecular biologic methods are being increasingly utilized and relied on in sarcoma pathology. These methods include chromosomal karyotyping, fluorescence in-situ hybridization, spectral karyotyping, and polymerase chain reaction– based methods for demonstrating specific chromosomal translocations and fusion genes. Understanding the basis of these methods and their application is critical to better provide accurate and validated specific diagnoses of sarcomas.

Reverse transcriptase in situ polymerase chain reaction for gene expression in rat mast cells and macrophages

1999 ◽  
Vol 226 (1-2) ◽  
pp. 147-158 ◽  
Author(s):  
O Nohara ◽  
M Gilchrist ◽  
R.E Déry ◽  
G.R Stenton ◽  
N.S Hirji ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Mast Cells ◽  
Reverse Transcriptase ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Rat Mast Cells
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