Genetic Analysis of Some Quantitative Traits in Hybrid Rice with Utilizing Cytoplasmic Genetic Male Sterility System

SUMMARYThe use of environmentally induced genetic male sterile (EGMS) rice could alter the development of hybrid rice from a three-line system to a two-line system. It is critical for the utilization of EGMS rice to determine which are the main environmental factors influencing fertility changes. Fertility responses to photoperiod (P) and temperature (T) were studied in 101 EGMS rice lines under nine controlled regimes combining three photoperiods (15·0, 14·0 and 12·5 h)x three temperatures (30·1, 24·1 and 23·1 °C). According to the variance analysis of seed-setting data, 96% of the total EGMS lines studied could be divided into three types as follows: (1) photoperiod-sensitive genetic male sterility (PGMS) characterized statistically by significant (P < 0·05) P and P × T interaction effects but by a non-significant T effect on fertility, (2) thermosensitive genetic male sterility (TGMS) by a significant T effect, a non-significant P effect and by either a significant or a non-significant P × T interaction effect on fertility, and (3) photo-thermosensitive genetic male sterility (P-TGMS) by only a significant P × T interaction effect on fertility. Among the japonica EGMS lines studied, PGMS, TGMS and P-TGMS accounted for 32·3, 9·7 and 51·6%, respectively. However, among the indica EGMS lines, no PGMS lines were detected and most of them were TGMS or P-TGMS (61·4 and 35·7%, respectively). The results indicate that the selection of indica PGMS lines of rice might be very difficult. The availability of different types of EGMS rice in two-line system hybrid rice is evaluated and the selection of an ideal model of response to photoperiod and temperature for indica EGMS is discussed.

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Developing rice hybrids for temperate conditions using three line approach

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.79.1.4 ◽

2019 ◽

Vol 79 (01) ◽

Author(s):

Amir Rashid ◽

Najeebul Rehman Sofi ◽

Asif Bashir Shikari ◽

Gazala Hassan Khan ◽

Showkat Ahmad Waza ◽

...

Keyword(s):

Grain Yield ◽

Male Sterility ◽

Pollen Fertility ◽

Hybrid Rice ◽

Fertility Restoration ◽

Kashmir Valley ◽

Cross Combination ◽

Yield Attributes ◽

Genetic Male Sterility ◽

Standard Heterosis

Exploitation of heterosis in the form of hybrid rice is yet to be exploited at commercial level in the temperate regions of India. The present study is attempted to develop rice hybrids for the temperate conditions of Kashmir valley by utilizing the cytoplasmic genetic male sterility system (CGMS). Among the four CMS lines evaluated, the genotype SKUA-7A and SKUA-11A had desirable performances. The 24 test crosses developed involving these CMS lines were evaluated for spikelet fertility and pollen fertility. Moreover, the highest standard heterosis for grain yield and other yield attributes over the check varieties (Jehlum and Shalimar Rice-3) was recorded in the cross combination K15-34 followed by K15-31 and K15-40. Pollen parents of all the three test crosses on screening with RM-6100, DDRMRf3-10 and DRCG-Rf4-14 markers revealed presence of both Rf3 and Rf4 genes responsible for fertility restoration. Besides, 39 genotypes were screened for fertility restoration and the lines RL-2, RL-5, RL-6, RL-7, RL-10, Pusa Sugandh3 and Kohsar were identified to carry homozygous alleles at both Rf3 and Rf4 loci. These genotypes can thus be used as promising restores for the development of temperate rice hybrids.

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Faculty Opinions recommendation of Genetic analysis of quantitative traits in the Japanese population links cell types to complex human diseases.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.732604093.793542393 ◽

2018 ◽

Author(s):

Anne Kwitek

Keyword(s):

Genetic Analysis ◽

Japanese Population ◽

Quantitative Traits ◽

Cell Types ◽

Human Diseases

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A Quantitative Genetic Analysis of Nuclear-Cytoplasmic Male Sterility in Structured Populations of Silene vulgaris

Genetics ◽

10.1093/genetics/158.2.833 ◽

2001 ◽

Vol 158 (2) ◽

pp. 833-841 ◽

Cited By ~ 2

Author(s):

Douglas R Taylor ◽

Matthew S Olson ◽

David E McCauley

Keyword(s):

Population Structure ◽

Sex Ratio ◽

Genetic Analysis ◽

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Sex Expression ◽

Silene Vulgaris ◽

Population Variance ◽

Quantitative Genetic ◽

Quantitative Genetic Analysis

Abstract Gynodioecy, the coexistence of functionally female and hermaphroditic morphs within plant populations, often has a complicated genetic basis involving several cytoplasmic male-sterility factors and nuclear restorers. This complexity has made it difficult to study the genetics and evolution of gynodioecy in natural populations. We use a quantitative genetic analysis of crosses within and among populations of Silene vulgaris to partition genetic variance for sex expression into nuclear and cytoplasmic components. We also use mitochondrial markers to determine whether cytoplasmic effects on sex expression can be traced to mitochondrial variance. Cytoplasmic variation and epistatic interactions between nuclear and cytoplasmic loci accounted for a significant portion of the variation in sex expression among the crosses. Source population also accounted for a significant portion of the sex ratio variation. Crosses among populations greatly enhanced the dam (cytoplasmic) effect, indicating that most among-population variance was at cytoplasmic loci. This is supported by the large among-population variance in the frequency of mitochondrial haplotypes, which also accounted for a significant portion of the sex ratio variance in our data. We discuss the similarities between the population structure we observed at loci that influence sex expression and previous work on putatively neutral loci, as well as the implications this has for what mechanisms may create and maintain population structure at loci that are influenced by natural selection.

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Molecular mapping of a thermosensitive genetic male sterility gene in rice using bulked segregant analysis

Genome ◽

10.1139/g97-027 ◽

1997 ◽

Vol 40 (2) ◽

pp. 188-194 ◽

Cited By ~ 59

Author(s):

P. K. Subudhi ◽

R. P. Borkakati ◽

S. S. Virmani ◽

N. Huang

Keyword(s):

Male Sterility ◽

Molecular Mapping ◽

Bulked Segregant Analysis ◽

Pcr Primers ◽

Rflp Markers ◽

Arbitrary Primers ◽

Repulsion Phase ◽

Genetic Male Sterility ◽

True Breeding ◽

Efficient Alternative

The thermosensitive genetic male sterility (TGMS) system is considered to be a more efficient alternative to the cytoplasmic male sterility (CMS) system for hybrid rice. An F2 population from a cross between a TGMS mutant line (IR32364TGMS) and IR68 was used to map the TGMS gene tms3(t). Fertile and sterile bulks were constructed following the classification of F2 plants into true breeding sterile, fertile, and segregating fertile plants based on F3 family studies. From the survey of 389 arbitrary primers in bulked segregant analysis, four RAPD markers were identified in which three, OPF182600, OPB19750, and OPAA7550, were linked to tms3(t) in repulsion phase and one, OPAC3640, was linked to tms3(t) in coupling phase. The tms3(t) gene was flanked by OPF182600 and OPAC3640 on one side and by OPAA7550 and OPB19750 on the other side. All four markers were low-copy sequences and two of them (OPF182600 and OPAC3640) detected polymorphism when the markers were used to probe the genomic blots. Subsequently, OPAC3640 was mapped to the short arm of chromosome 6 using a mapping population available at IRRI. However, no RFLP markers from this region showed linkage to tms3(t) owing to the lack of polymorphism between the parents. All RAPD fragments were cloned and partially sequenced from both ends. Thus, PCR primers can be designed to develop PCR markers for marker-assisted breeding to facilitate the transfer of tms3(t) from one genetic background to another.Key words: bulked segregant analysis, gene tagging, marker-assisted selection, RAPD, TGMS.

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