Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of Silene vulgaris Suggest the Importance of Oxidative Stress in Pollen Abortion

Cytoplasmic male sterility (CMS), encoded by the interacting mitochondrial and nuclear genes, causes pollen abortion or non-viability. CMS is widely used in agriculture and extensively studied in crops. Much less is known about CMS in wild species. We performed a comparative transcriptomic analysis of male sterile and fertile individuals of Silene vulgaris, a model plant for the study of gynodioecy, to reveal the genes responsible for pollen abortion in this species. We used RNA-seq datasets previously employed for the analysis of mitochondrial and plastid transcriptomes of female and hermaphrodite flower buds, making it possible to compare the transcriptomes derived from three genomes in the same RNA specimen. We assembled de novo transcriptomes for two haplotypes of S. vulgaris and identified differentially expressed genes between the females and hermaphrodites, associated with stress response or pollen development. The gene for alternative oxidase was downregulated in females. The genetic pathways controlling CMS in S. vulgaris are similar to those in crops. The high number of the differentially expressed nuclear genes contrasts with the uniformity of organellar transcriptomes across genders, which suggests these pathways are evolutionarily conserved and that selective mechanisms may shield organellar transcription against changes in the cytoplasmic transcriptome.

A study on wild edible plants for human consumption in Hizan county of Bitlis, Turkey

This study covers the edible plants in Hizan where there is a rich culture of plant consumption. The study was conducted between 2018 and 2019 to record the culture of traditional food plant use of the local people in Hizan. In this regard, the face-to-face interviews with the local people were made, and the relevant plants used were collected and identified. A total of 65 species of wild edible plants belonging to 24 families were identified, and their different traditional usages were categorized as cooked, cheese making, rennet, specie and gum producing, and as raw or beverages etc. In addition, the use-value (VU) index was calculated for each species. The mostly used species included Rheum ribes L. (UV: 0.70), Gundelia tournefortii L. (0.66), Silene vulgaris (Moench) Garcke, Rosa canina L. (0.64), Urtica dioica L. (0.63), Malva neglecta Wallr. (0.61), and Pistacia khinjuk Stocks (0.60). The culture of edible plant use is widespread throughout the Anatolia. However, traditional uses of many wild plants have not been recorded yet. Therefore, this research will be an important contribution to the preservation of the cultural heritage associated with traditional wild edible plants in this region. Bangladesh J. Plant Taxon. 27(2): 377-389, 2020 (December)

Activity and Diversity of Microorganisms in Root Zone of Plant Species Spontaneously Inhabiting Smelter Waste Piles

The aim was to assess plant driven changes in the activity and diversity of microorganisms in the top layer of the zinc and lead smelter waste piles. The study sites comprised two types (flotation waste—FW and slag waste—SW) of smelter waste deposits in Piekary Slaskie, Poland. Cadmium, zinc, lead, and arsenic contents in these technosols were extremely high. The root zone of 8 spontaneous plant species (FW—Thymus serpyllum, Silene vulgaris, Solidago virgaurea, Echium vulgare, and Rumex acetosa; and SW—Verbascum thapsus; Solidago gigantea, Eupatorium cannabinum) and barren areas of each waste deposit were sampled. We observed a significant difference in microbial characteristics attributed to different plant species. The enzymatic activity was mostly driven by plant-microbial interactions and it was significantly greater in soil affected by plants than in bulk soil. Furthermore, as it was revealed by BIOLOG Ecoplate analysis, microorganisms inhabiting barren areas of the waste piles rely on significantly different sources of carbon than those found in the zone affected by spontaneous plants. Among phyla, Actinobacteriota were the most abundant, contributing to at least 25% of the total abundance. Bacteria belonging to Blastococcus genera were the most abundant with the substantial contribution of Nocardioides and Pseudonocardia, especially in the root zone. The contribution of unclassified bacteria was high—up to 38% of the total abundance. This demonstrates the unique character of bacterial communities in the smelter waste.

Nutritional and Phytochemical Composition of Mediterranean Wild Vegetables after Culinary Treatment

Studies are scarce on the nutritional and phytochemical composition of wild edible Mediterranean plants after culinary processing. This work provides the nutritional composition after culinary treatment (including dietary fiber and mineral composition) and bioactive compounds (folates, vitamin C and organic acids) of wild Rumex pulcher L., Silene vulgaris (Moench) Garcke. leaves, and wild Asparagus acutifolius L., Bryonia dioica Jacq., Humulus lupulus L., Tamus communis L. young shoots. Shoots better preserved their nutrients than leaves, due to their different tissue structure. Fresh and cooked wild greens present high dietary fiber values, and remained at remarkable levels after boiling. Na, K, Mg and Zn were lost in about 50% due to culinary processing, while Ca, Cu, Fe and Mn were more stable. Boiled leaves of S. vulgaris remained as a good Mn source. A portion of 100 g of most of the cooked analyzed species could cover a relevant percentage of the daily requirement of folates (R. pulcher and A. acutifolius providing more than 80%) and vitamin C (T. communis and A. acutifolius providing more than 35%).

DESCRIPTIONOFSEXUALESOFBRACHYCOLUSCUCUBALI(PASSERINI,1863)(HEMIPTERAAPHIDIDAE)

The hitherto unknown oviparous females and apterous males of Brachycolus cucubali(Passerini,1863), living in pseudogalls on Silene vulgaris(Moench) Garcke, (1869) (Caryophyllaceae), are described based on material from the North-West of Iberian Peninsula (Province of León). Sampling and morphometric data are given for every morph. Also, field data of monitored Brachycolus cucubali colonies are reported and information of polyphenismin males is discussed.

Four decades of new vascular plant records for Greenland

Records of new species of vascular plants in Greenland from the last four decades are presented and new phytogeographical data leading to extension of the known distribution limits in Greenland are discussed. Since the publication of the latest edition of the Flora of Greenland in 1978 (Böcher et al. 1978) fieldwork by Greenland Botanical Survey and other expeditions have taken place especially in West and East Greenland and in many remote areas in North and Northeast Greenland. This paper serves as an update of the Flora of Greenland. Twenty species, one subspecies and one new forma have been added to the flora of Greenland: Carex membranacea Hook., Carex miliaris Michx., Carex rhomalea (Fernald) Mack., Equisetum hyemale L., Festuca edlundiae S. Aiken, Consaul and Lefkovich, Festuca groenlandica (Schol.) Frederiksen, Festuca saximontana Rydb., Galium verum L., Geum rossii (R. Br.) Ser., Papaver cornwallisense D. Löve, Papaver dahlianum Nordh., Papaver labradoricum (Fedde) Solstad and Elven, Papaver lapponicum (Tolm.) Nordh., Pedicularis sudetica Willd. ssp. albolabiata Hult., Poa flexuosa Sm., Puccinellia bruggemanni Th. Sør., Ranunculus subrigidus W.B. Drew., Silene vulgaris (Moench) Garcke, Trientalis europaea L. and Veronica officinalis L. in addition to one subspecies Phippsia algida (Sol.) R. Br. ssp. algidiformis (H. Sm.) Löve and Löve. The viviparous form of Poa hartzii f. prolifera has been reported for the first time in Greenland. Presently, the total number of vascular plant species in Greenland is 532. 89 new northern and 28 new southern distribution limits are presented and 26 species are new to the flora province East Greenland, whereas 15 species are new to West Greenland. The numbers of new species to flora provinces North and South Greenland are 14 and one, respectively.