scholarly journals Direct Shoot Bud Proliferation Protocol from Stevia rebaudiana Leaf Culture for Healthy Biomass Production

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Ahmed Saad Attaya
Keyword(s):  
Biomass Production ◽  
Stevia Rebaudiana ◽  
Leaf Culture ◽  
Shoot Bud ◽  
Direct Shoot

scholarly journals Influencia de la materia orgánica y Azotobacter nigricans en un cultivo de Stevia rebaudiana B

2011 ◽  
Vol 16 (3) ◽  
pp. 282 ◽  
Author(s):  
Daniel Borda-Molina ◽  
Juan Manuel Pardo-García ◽  
José Salvador Montaña-Lara ◽  
María Mercedes Martínez-Salgado
Keyword(s):  
Organic Carbon ◽  
Biomass Production ◽  
Organic Waste ◽  
Stevia Rebaudiana ◽  
Dry Weight ◽  
Physicochemical Analysis ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Biomass Increase

<p><strong> </strong></p> <p><strong> </strong><em>Stevia rebaudiana</em> is a plant that accumulates a non-caloric sweetener compound known as stevioside. This crop is traditionally fertilized with chemicals that are harmful for the ecosystem, forcing to find  organic alternatives to mitigate this damage. <strong>Objective</strong>. To study the effect of organic matter and an <em>Azotobacter nigricans</em>-based bio-fertilizer on a <em>Stevia rebaudiana </em>plantation grown in acidic soil in the Department of Meta, Colombia. <strong>Materials and methods.</strong> Five treatments were established: T1 and T2 with the application of home organic waste compost at concentrations of 15 and 30 ton ha<sup>-1</sup>. T3 and T4 with the same compost concentrations and inoculating the<em> A. nigricans </em>bio-fertilizer. T5 contained the bio-fertilizer alone. The control consisted of the application of the Bokashi compost under the usual conditions of cultivation.  Plant growth was assessed by biomass increase measured as dry weight, production, and leaf area.  The physicochemical analysis of soil included: percentage of organic carbon, water content, and pH. <strong>Results. </strong>The inoculation of the bio-fertilizer produced an increase in the rate of mineralization of compost, reaching a final 4.85% of OC between 90 and 180 days after inoculation. <strong> </strong>There were significant (p&lt; 0.05) differences between biomass production with T2 (1,538 kg ha<sup>-1</sup>) and the control (477 kg ha<sup>-1</sup>).  Regarding the soluble solid content, T1, T3 and T4 showed the highest °Brix values (12.4, 12.35 and 12.15, respectively).  <strong>Conclusions</strong>. The concentration of 30 ton ha<sup>-1</sup> produced the highest biomass production and the application of the biofertilizer showed a positive correlation with compost mineralization and glucoside synthesis.</p> <p><strong> </strong></p> <p><strong>Key words</strong>: <em>Stevia rebaudiana</em>, bio-fertilizer, mineralization, organic carbon.</p> <p> </p><br />

In vitro studies on direct shoot regeneration of Stevia rebaudiana using nodal explants

Crop Research ◽  
2017 ◽  
Vol 52 (6) ◽  
pp. 259
Author(s):  
Puneet Beniwal ◽  
Priyanka Siwach ◽  
Vijay S. Beniwal ◽  
Vikas Hooda
Keyword(s):  
Shoot Regeneration ◽  
Stevia Rebaudiana ◽  
In Vitro Studies ◽  
Nodal Explants ◽  
Direct Shoot Regeneration ◽  
Direct Shoot

Direct shoot regeneration from immature inflorescence cultures of Chlorophytum arundinaceum and Chlorophytum borivilianum

Biologia ◽  
2009 ◽  
Vol 64 (2) ◽  
Author(s):  
Sanghamitra Samantaray ◽  
Saroj Kumar ◽  
Satyabrata Maiti
Keyword(s):  
Shoot Regeneration ◽  
Terminal Segment ◽  
Type I ◽  
Ms Medium ◽  
Direct Shoot Regeneration ◽  
Immature Inflorescence ◽  
Shoot Buds ◽  
Shoot Bud ◽  
Half Strength ◽  
Direct Shoot

AbstractDirect shoot regeneration was achieved from immature inflorescence explants of Chlorophytum arundinaceum and C. borivilianum on half-strength Murashige & Skoog (MS) medium supplemented with 3.0 mg L−1 BA, 150 mg L−1 Ads, 0.1 mg L−1 NAA and 3% (w/v) sucrose under a 16-h photoperiod. The shoot buds developed within 2–3 weeks of culture. High frequency of shoot bud regeneration was achieved when cultured on similar medium in subsequent subcultures. The apex portion (Type I) of the inflorescence produced more shoot buds as compared to the middle ones (type II). More than 75% of the terminal segment explants produced shoot buds within 4-week of culture. Response of basal portion (Type III) was negative for shoot bud initiation. Shoots rooted on half-strength basal MS medium supplemented with half-strength MS medium, 0.1 mg L−1 IAA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the green house and successfully established in the soil where 90% of the plants survived. This protocol would be useful for commercial micropropagation and genetic improvement prograrmme.

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