Influence of fish oil and antioxidants supplementation on carcass traits, immune response, minerals and amino acid composition of broiler meat

By applying the concept of ‘surface-simulation’ synthesis to the combining site of the myeloma protein M-603 we were able to mimic synthetically its phosphorylcholine-binding characteristics. The synthetic surface-simulation peptide was found to bind to phosphorylcholine, whereas a control peptide that had the same amino acid composition but a different sequence showed little or no binding activity. The specificity of the binding was further confirmed by inhibition studies in which the surface-simulation peptide, but not the control peptide, inhibited the binding of 125I-labelled surface-simulation peptide to phosphorylcholine. Furthermore, the surface-simulation peptide was found to completely inhibit the binding of the native myeloma protein, M-603, to phosphorylcholine. The control peptide was unable to inhibit this binding. These findings suggest that surface-simulation synthesis can be effectively employed to mimic synthetically antibody combining sites, and may in the future be a valuable tool with which to manipulate the immune response to clinically important antigens.

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Amino-acid composition of broiler meat in the application of feed additives “Mikofiks”

RUDN Journal of Agronomy and Animal Industries ◽

10.22363/2312-797x-2016-2-51-57 ◽

2016 ◽

pp. 51-57

Author(s):

M.V. Zabolotnykh ◽

◽

A.A. Dikikh ◽

I.G. Seryogin ◽

V.E. Nikitchenko ◽

...

Keyword(s):

Amino Acid ◽

Amino Acid Composition ◽

Acid Composition ◽

Feed Additives ◽

Broiler Meat

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Genomic evolution of epitopes and Low Complexity Regions in Plasmodium

10.1101/2021.01.29.428855 ◽

2021 ◽

Author(s):

Sarah N. Medley ◽

Alyssa Beaudet ◽

Helen Piontkivska ◽

Fabia U. Battistuzzi

Keyword(s):

Immune Response ◽

Amino Acid ◽

Amino Acid Composition ◽

Acid Composition ◽

Plasmodium Species ◽

Low Complexity ◽

Host Immune Response ◽

Sequence Conservation ◽

Evolutionary Mechanisms ◽

Structural Order

AbstractDespite decades-long efforts to eradicate malaria, pathogens genomic complexity and variability continue to pose major challenges for the vaccine and drug development. Here we examined the evolutionary history of epitopes and epitope-like regions to determine whether they share underlying evolutionary mechanisms and potential functions that are relevant to pathogens interactions with the host immune response. Our comparative sequence analyses contrasted patterns of sequence conservation, amino acid composition, and protein structure of epitopes and low complexity regions (LCRs) in 21 Plasmodium species. Our results revealed many similarities in amino acid composition and preferred secondary structures between epitopes and LCRs; however, we also identified differences in evolutionary trends where LCRs exhibit overall lower sequence conservation and higher disorder. We also found that both epitopes and LCRs have a wide array of configurations, with various levels of sequence conservation and structural order. We propose that such combination of different levels of conservation and structural order between epitopes and LCRs in the same gene play a role in maintaining the functional integrity required by the pathogen along with the variability necessary to evade the host immune response, with LCRs playing a role in the evasion particularly in the vicinity of conserved epitopes. Overall, our results suggest that there are at least two categories of LCRs, where some LCRs play a potential protective role for conserved (ordered) epitopes because of their variable (or disordered) sequence, while others are less disordered and are as conserved as epitopes. The former ones may be an evolutionary necessity for Plasmodium to maintain the diversity of epitopes, while the latter category may serve currently unknown function(s) and deserve to be examined in greater detail. Our findings show that there may be many more candidate targets for future anti-malarial treatments than initially thought and that some of these targets may work across strains and species.

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Ultrafine silica dioxide alters amino acid composition of broiler meat and liver

Animal Husbandry and Fodder Production ◽

10.33284/2658-3135-103-3-8 ◽

2020 ◽

Vol 103 (3) ◽

pp. 8-15

Author(s):

Alexandra S Mustafina ◽

Keyword(s):

Amino Acid ◽

Amino Acid Composition ◽

Acid Composition ◽

Broiler Meat ◽

Ultrafine Silica ◽

Silica Dioxide

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Effect of Sauropus androgynus Leaf Extract and Fish Oil Plus Vitamin E on Performance, Carcass Quality, and Meat Amino Acid Composition in Broiler Chickens

Jurnal Agripet ◽

10.17969/agripet.v21i2.18633 ◽

2021 ◽

Vol 21 (2) ◽

pp. 105-112

Author(s):

Urip Santoso ◽

Yosi Fenita ◽

Kususiyah Kususiyah

Keyword(s):

Vitamin E ◽

Amino Acid ◽

Fish Oil ◽

Amino Acid Composition ◽

Acid Composition ◽

Leaf Extract ◽

Amino Acid Content ◽

Feed Consumption ◽

Spleen Weight ◽

Carcass Quality

ABSTRACT. This study aimed to evaluate the effect of Sauropus androgynus leaf extract (SALE), lemuru fish oil and vitamin E supplementation on performance, carcass quality, and amino acid composition of broiler meat. One hundred and ninety-five broilers aged 21 days were distributed into 13 groups as follows: broilers were fed diet with 0.5% commercial supplement feed (P0), 10 g/kg SALE plus 1% lemuru fish oil (LFO) (P1), 10 g SALE/kg and LFO 1% plus 60 mg vitamin E (P2), 10 g SALE/kg and LFO 2% (P3), 10 g SALE/kg and LFO 2% plus 60 mg vitamin E (P4), 10 g SALE/kg and LFO 3% (P5), 10 g SALE/kg and LFO 3% plus 60 mg vitamin E (P6), 18 g SALE/kg and LFO 1% (P7), 18 SALE g/kg and LFO 1 % plus 60 mg vitamin E (P8), 18 g SALE/kg and FLO 2% (P9), 18 SALE g/kg and FLO 2% plus vitamin E (P10), 18 g SALE/kg and LFO 3% (P11 ), and 18 g SALE/kg and LFO 3% plus 60 mg vitamin E. The variables measured were performance, carcass quality, organoleptic properties, protein and amino acid composition of meats. The experimental results showed that the treatment had no significant effect (P 0.05) on body weight, feed consumption, feed conversion ratio, carcass weight, meat color, meat odor, meat taste, and cooking losses. However, the treatment had a significant effect (P 0.05) on carcass color, spleen weight, and protein content. In addition, the treatment also changes the amino acid composition of the meat. In conclusion, supplementation of 18 g SALE/kg diet, 3% LFO plus vitamin E resulted in meat with the highest protein and amino acid content. (Pengaruh ekstrak daun katuk, minyak ikan plus vitamin E terhadap performa, kualitas karkas, dan komposisi asam amino pada broiler) ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi pengaruh suplementasi ekstrak daun katuk (EDK), minyak ikan lemuru, dan vitamin E terhadap performa, kualitas karkas, dan komposisi asam amino daging broiler. Seratus sembilan puluh lima ekor broiler umur 21 hari didistribusikan ke dalam 13 kelompok sebagai berikut: broiler diberi pakan mengandung 0,5% pakan imbuhan komersial (P0), 10 g/kg EDK plus 1% minyak lemuru (MIL) (P1), EDK 10 g/kg dan MIL 1% plus 60 mg vitamin E (P2), EDK 10 g/kg dan MIL 2% (P3), EDK 10 g/kg dan MIL 2% plus 60 mg vitamin E (P4), EDK 10 g/kg dan MIL 3% (P5), EDK 10 g/kg dan MIL 3% plus 60 mg vitamin E (P6), EDK 18 g/kg dan MIL 1% (P7), EDK 18 g/kg dan MIL 1% plus 60 mg vitamin E (P8), EDK 18 g/kg dan MIL 2% (P9), EDK 18 g/kg dan MIL 2% plus vitamin E (P10), EDK 18 g/kg dan MIL 3% (P11), dan EDK 18 g/kg dan MIL 3% plus 60 mg vitamin E. Variabel yang diukur adalah performa, kualitas karkas, dan sifat organoleptik, protein dan komposisi asam amino daging. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh tidak nyata (P0,05) terhadap berat badan, konsumsi pakan, konversi pakan, berat karkas, warna daging, bau daging, rasa daging, dan susut masak. Akan tetapi perlakuan berpengaruh nyata (P0,05) terhadap warna karkas, berat limfa, kadar protein. Selain itu, perlakuan juga mengubah komposisi asam amino daging. Kesimpulannya, suplementasi 18 g EDK/kg pakan, 3% MIL plus vitamin E menghasilkan daging dengan kandungan protein dan asam amino tertinggi.

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