Application of plant growth regulators as a method for realization the production potential of table grapes in the conditions of Pridnestrovie

Изучено влияние физиологически активных веществ Гиббереллина, Циркона и Эпин-экстра на механический состав грозди, урожайность и содержание сахаров в соке ягод трех столовых сортов винограда в условиях Приднестровья. Определено влияние физиологически активных веществ на изменение массы грозди, количества ягод в грозди и урожайности в зависимости от метеорологических условий года. Установлено, что в более влажных условиях 2019 г. (ГТК = 1,0) увеличение массы грозди в опытных вариантах в сравнении с контрольными растениями составило на сорте Золотой Дон 24,5-43,5%; на сорте Велика - 48,4-81,0%. В менее влагообеспеченном 2020 г. (ГТК = 0,6) это превышение было на уровне - 53,3-110,3% и 17,7-41,7% соответственно. Увеличение массы грозди у сортов винограда в вариантах с обработкой регуляторами роста растений наблюдается преимущественно вследствие повышения количества ягод. Выявлено, что двукратная обработка растений физиологически активными веществами приводит к значительному снижению ягодного показателя и росту показателя строения грозди винограда. Оптимальным вариантом двукратной обработки растений сортов столового направления использованиия являются: для сорта Золотой Дон - Циркон, 0,6 мл/л; для сорта Велика - Эпин-экстра, 3,2 мл/л, в более увлажненных условиях 2019 г., что позволило повысить урожайность кустов на 46,7 и 81,1% соответственно. В засушливых условиях 2020 г. обработка сорта Золотой Дон препаратом Эпин-экстра в концентрации 3,2 мл/л и сорта Велика Цирконом, 0,6 мл/л, была наиболее эффективной, увеличив урожайность в 1,5-2,0 раза по сравнению с контролем. The effect of plant growth regulators Gibberellin, Zircon and Epin-extra on mechanical composition of the bunch, cropping capacity and sugar content in the juice of berries of three table grape varieties in the conditions of Pridnestrovie was studied. The influence of physiologically active substances on changes in the bunch weight, number of berries per bunch and cropping capacity depending on the meteorological conditions of the year was determined. It was found that in the more humid conditions of 2019 (HTC of 1.0), the bunch weight increase in experimental variants in comparison with the control plants was from 24.5% to 43.5% in ‘Zolotoy Don’ grape variety, and from 48.4% to 81.0% in ‘Velika’ grape variety. In the less moisture-rich 2020 (HTC of 0.6) this exceeding was at the level of 53.3%-110.3% and 17.7%-41.7%, respectively. An increase in the bunch weight of grape varieties treated with plant growth regulators is mainly observed due to an increase in the number of berries. It was found that two-fold treatment of plants with physiologically active substances leads to a significant decrease in the berry index and an increase in the index of grape bunch structure. The best variant for two-fold treatment of plants is Zircon, 0.6 ml/l, - for ‘Zolotoy Don’ variety; Epin-extra, 3.2 ml/l, - for ‘Velika’ grape variety in more humid conditions of 2019, resulting in the increase in cropping capacity of bushes by 46,7% and 81.1%, respectively. In the arid conditions of 2020, the treatment of ‘ Zolotoy Don’ variety with Epin-extra at a concentration of 3.2 ml/l and ‘Velika’ variety with Zircon, 0.6 ml/l, was the most effective, increasing the yield by 1.5-2.0 times compared to the control.

INFLUENCE OF PHYSIOLOGICALLY ACTIVE SUBSTANCES ON DEVELOPMENT OF OWN-ROOTED SEEDLINGS IN NURSERY

The purpose of the research was to identify growth stimulators that promote the activa-tion of growth processes and root formation, to improve the technology of growing high-quality own-rooted grape seedlings from shortened three-eyed cuttings. The influence of physiologically active substances (PAS): Epin, Silk, Kornevin, Nikfan, Kavkaz on sur-vival, activation of growth processes, root formation of shortened cuttings of Bianca grapes was determined. As a result of pro-cessing cuttings with PAS, the survival rate of cuttings in the nursery increased by 10.6–19.1 % relative to the control. The yield of seedlings of the variety in the experimental variants exceeded the control indicators by 2.8–18.4 %. The effect of preparations on plant growth was noted, the increase in one-year growth in the variants varied from 5.8 to 18.0 cm relative to the control. When the ba-sal part of the cuttings was briefly immersed in a 0.01 % solution of Nikfan, the growth length was 78.1 cm, when soaked in a 0.1 % solution of Kornevin – 76.8 cm, in a 0.1 % solution of Silk –75.5 cm, in the control this indicator was 60.1 cm. The positive effect of growth stimulants on the development of the root system has been established. The indica-tor of the average number of roots per own-rooted seedling for the variants varied from 10.5 to 12.5 pcs., in the control variant – 10.7 pcs. According to the complex of indi-cators, a variant with the treatment of the ba-sal part of the cuttings for 24 hours with the preparation Kornevin-0.1% was identified.

A Comprehensive Overview of Novel Hydroxynitrile Lyases

Hydroxynitrile lyases (HNLs) are a heterogeneous family of enzymes that are of particular interest because of their structurally unique categories, a wide range of immobilisation techniques and procedures, and a wide range of sources with varying degrees of enantiopurity and enantioselectivity. Cupin, which contains a new type of HNL from bacteria, the lipocalin superfamily, which has HNLs from millipedes, and the + barrel fold superfamily, which contains HNL from a fern, have all been discovered in recent decades. Their biochemistry has been deciphered, and engineering efforts have been made to boost their productivity, purity, and activity. These remarkable enzymes opened up a new vista in the field of industrial catalysts since they are actively used in the synthesis of crucially important agrochemicals, medicines, physiologically active substances, and chemo-enzymatic follow-up procedures. This review focuses on recent advances, evolutionary history, and recombinant engineering of HNL from the previous decade.

Comparative study of photosynthetic pigments in the leaves of forage plants of the cryolithozone of Yakutia

Thin-layer chromatography and spectrophotometry were used to determine the content of photosynthetic pigments (chlorophylls a and b, carotenoids) in green leaves of forage plants. It was found that the content of carotenoids was high for forage grasses with a high content of green pigments. It was revealed that β-carotene plays the most important role since it is a precursor of vitamin A and lutein, which has a high antioxidant (protective) property. According to modern concepts, lutein and β-carotene actively function in various organs of herbivores. They play a special role in the functioning of the mammalian visual apparatus, protecting this organ from the light factor. In addition, lutein is one of the physiologically active substances that make up cow’s milk.

Potential Roles of Exosomal lncRNAs in the Intestinal Mucosal Immune Barrier

The intestinal mucosal immune barrier protects the host from the invasion of foreign pathogenic microorganisms. Immune cells and cytokines in the intestinal mucosa maintain local and systemic homeostasis by participating in natural and adaptive immunity. Deficiency of the intestinal mucosal immune barrier is associated with a variety of intestinal illnesses. Exosomes are phospholipid bilayer nanovesicles that allow cell-cell communication by secreting physiologically active substances including proteins, lipids, transcription factors, mRNAs, micro-RNAs (miRNAs), and long noncoding RNAs (lncRNAs). Exosomal lncRNAs are involved in immune cell differentiation and the modulation of the immune response. This review briefly introduces the potential role of exosomal lncRNAs in the intestinal mucosal immune barrier and discusses their relevance to intestinal illnesses.

Human Vascular Smooth Muscle Function and Oxidative Stress Induced by NADPH Oxidase with the Clinical Implications

Among reactive oxygen species, superoxide mediates the critical vascular redox signaling, resulting in the regulation of the human cardiovascular system. The reduced form of nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase, NOX) is the source of superoxide and relates to the crucial intracellular pathology and physiology of vascular smooth muscle cells, including contraction, proliferation, apoptosis, and inflammatory response. Human vascular smooth muscle cells express NOX1, 2, 4, and 5 in physiological and pathological conditions, and those enzymes play roles in most cardiovascular disorders caused by hypertension, diabetes, inflammation, and arteriosclerosis. Various physiologically active substances, including angiotensin II, stimulate NOX via the cytosolic subunits’ translocation toward the vascular smooth muscle cell membrane. As we have shown, some pathological stimuli such as high glucose augment the enzymatic activity mediated by the phosphatidylinositol 3-kinase-Akt pathway, resulting in the membrane translocation of cytosolic subunits of NOXs. This review highlights and details the roles of human vascular smooth muscle NOXs in the pathophysiology and clinical aspects. The regulation of the enzyme expressed in the vascular smooth muscle cells may lead to the prevention and treatment of human cardiovascular diseases.

Weed Control and Winter Wheat Crop Yield With the Application of Herbicides, Nitrogen Fertilizers, and Their Mixtures With Humic Growth Regulators

The aim of the present study was to determine the efficacy of the application of mixtures containing various combinations of humic substances, with herbicides and nitrogen fertilizers, in weed control and optimizing the plant nutrition system. We also aimed to evaluate the influence of these substances on winter wheat productivity. Five Ukrainian winter wheat cultivars (‘Kryzhynka,’ ‘Smuhlyanka,’ ‘Slavna,’ ‘Kubus,’ and ‘Mulan’) were sown in a randomized complete block design, with three replications, in the years 2014–2019. The analysis of the effect of the compositions containing herbicides, with various physiologically active substances, in a mixture with humic preparations (Humifield, 4R Foliar concentrate) was performed by counting weeds per square meter in each experimental plot. The best performance in weed control, including perennial species, was obtained from using a mixture of Grodil Maxi herbicide with the humic preparation, Humifield. The crop treatment of this mixture resulted in a 23.6% reduction in weeds, compared to the treatment with the Grodil Maxi herbicide only. At the same time, the complex application of a number of herbicides in a mixture with the humic preparation, 4R Foliar concentrate led to the opposite effect. Various applications of mixtures of humates (4R Foliar concentrate, 5R SoilBoost) with nitrogen fertilizers (ammonium nitrate; carbamide-ammonium mixture) to optimize the winter wheat nutritional system and yield increases have been studied. The highest yield increase of 20%–22% was harvested in the plots treated with 5R SoilBoost and 4R Foliar concentrate plus ammonium nitrate. In addition, the efficacy of wheat crop foliar feeding with mixtures of humates, plus a carbamide-ammonia mixture, in different phases of vegetation has been established. A yield increase of 10.0%–21.4% resulting from the use of such compositions was obtained.

Current Perspectives on the Physiological Activities of Fermented Soybean-Derived Cheonggukjang

Cheonggukjang (CGJ, fermented soybean paste), a traditional Korean fermented dish, has recently emerged as a functional food that improves blood circulation and intestinal regulation. Considering that excessive consumption of refined salt is associated with increased incidence of gastric cancer, high blood pressure, and stroke in Koreans, consuming CGJ may be desirable, as it can be made without salt, unlike other pastes. Soybeans in CGJ are fermented by Bacillus strains (B. subtilis or B. licheniformis), Lactobacillus spp., Leuconostoc spp., and Enterococcus faecium, which weaken the activity of putrefactive bacteria in the intestines, act as antibacterial agents against pathogens, and facilitate the excretion of harmful substances. Studies on CGJ have either focused on improving product quality or evaluating the bioactive substances contained in CGJ. The fermentation process of CGJ results in the production of enzymes and various physiologically active substances that are not found in raw soybeans, including dietary fiber, phospholipids, isoflavones (e.g., genistein and daidzein), phenolic acids, saponins, trypsin inhibitors, and phytic acids. These components prevent atherosclerosis, oxidative stress-mediated heart disease and inflammation, obesity, diabetes, senile dementia, cancer (e.g., breast and lung), and osteoporosis. They have also been shown to have thrombolytic, blood pressure-lowering, lipid-lowering, antimutagenic, immunostimulatory, anti-allergic, antibacterial, anti-atopic dermatitis, anti-androgenetic alopecia, and anti-asthmatic activities, as well as skin improvement properties. In this review, we examined the physiological activities of CGJ and confirmed its potential as a functional food.

The Applying chemical control to suppress liverwort (Marсhantia polymorpha L.) and other mosses when growing containerized seedlings of pine and spruce

The spread of bryophytes (liverworts and green mosses) on the substrate surface in containers and cassettes poses a significant problem when growing containerized coniferous seedlings. It requires a complex control programme to significantly minimize the negative effect which mosses, in particular Marсhantia polymorpha L., pose to the growth of containerized pine and spruce seedlings. In addition to the preventive and agrotechnical measures, this programme should also involve application of the physiologically active substances. North American and European nurseries have faced this problem for a long time, Russian nurseries have started to experience it only in recent years due to increased output of containerized pine and spruce seedlings grown in greenhouses. In this paper, we assessed the effectiveness of some herbicides for moss control and their selectivity to pine and spruce seedlings of different ages. The following chemicals were applied in the tests: Goal 24% EC (a.i. oxyfluorfen), Stomp 33% EC (a.i. pendimethalin), Velpar 90% SP (a.i. hexazinone), Pledge 25% WP (a.i. flumioxazin), Mogeton 25% WP (a.i. quinoclamine), Granstar 75% WDG (a.i. tribenuron-methyl), Anchor-85 75% WDG (a.i. sulfometuron methyl) as well as cinnamon oil and baking soda. The experiments were conducted in greenhouses and outdoor fields. It was found that the pre-emergent (before the pine and spruce shoots appear) application of Mogeton WP and baking soda in the greenhouse resulted in the effective suppression of green mosses for up to 20 weeks after the treatment, without any signs of injury in seedlings. Under the same conditions, Goal EC, Stomp EC, Pledge WP and their mixtures in different combinations, as well as Velpar SP caused significant damage to pine and spruce seedlings. The post-emergent treatment by Velpar SP, Pledge WP, Mogeton WP, Granstar WDG, Anchor-85 WDG, as well as cinnamon oil and baking soda, provided effective and long-term control of liverwort and green mosses in cassettes without damage to the seedlings. Key words: Marchantia polymorpha L., pine, spruce, efficacy, selectivity