In vitro, induction of salt tolerant potato (solanum tuberosum l.) Plants with gamma irradiation and characterization of genetic variations through sds-page and issr-pcr analysis

Genetic transformation using nodal and internodal segments from three economically important potato (Solanum tuberosum L.) varieties namely, Diamant, Cardinal and Granola was conducted using an Agrobacterium tumefaciens strain LBA4404 harbouring binary plasmid pBI12 containing the GUS and nptII genes. Node and internodal segments were used for direct regeneration as well as regeneration with the intervention of callus. best responses were obtained for direct regeneration of shoots when the explants were cultured on MS supplemented with 4.0 mg/l BAP +1.0 mg/l IAA, 1.5 mg/l BAP + 0.5 mg/l IAA and 5.0 mg/l BAP +1.0 mg/l IAA in Diamant, Cardinal and Granola, respectively. In Diamant spontaneous in vitro microtuberization was obtained from these proliferated shoots. Further culturing of these in vitro grown green microtubers regenerated a large number of shoots on MS containing 4.0 mg/l BAP +1.0 mg/l IAA. By combining the best treatments, this protocol yielded an average transformation rate of 87% of treared explants. Stable expression of GUS gene was visualized in the various parts of transformed shoots through histochemical assay. Genomic DNA was isolated from transformed shoots and stable integration of the GUS and nptII genes was confirmed by PCR analysis. Key words: Potato, in vitro regeneration, transformation D.O.I. 10.3329/ptcb.v20i2.6894 Plant Tissue Cult. & Biotech. 20(2): 145-155, 2010 (December)

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In vitro Regeneration and Agrobacterium-mediated Transformation of Potato (Solanum tuberosum L.) Cultivars Grown in Mexico

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v22i2.14193 ◽

2013 ◽

Vol 22 (2) ◽

pp. 93-105 ◽

Cited By ~ 1

Author(s):

Rose Onamu ◽

Juan P Legaria ◽

Jaime C Sahagún ◽

José L Rodríguez ◽

Joel N Pérez

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L ◽

In Vitro Regeneration ◽

Potato Cultivars ◽

Pcr Analysis ◽

Nptii Gene ◽

Histochemical Assay ◽

Gus Histochemical Assay ◽

Binary Plasmid

Prior to Agrobacterium-mediated genetic transformation in vitro regeneration protocol was established for three potato cultivars (Alfa, Cambray Rosa Morelos and Atlantic) grown in Mexico using leaf, node and internodal explants. Regeneration protocol was developed with or without the intervention of callus. Two potato cultivars, namely, Cambray Rosa Morelos and Alpha were transformed using Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121 containing the GUS and nptII genes. GUS histochemical assay and PCR analysis were conducted on rooted shoots grown in media without hormones but supplemented with antibiotics. Transformed shoots tested positive through GUS histochemical assay and integration of nptII gene was confirmed by PCR analysis DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14193 Plant Tissue Cult. & Biotech. 22(2): 93-105, 2012 (December)

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In vitro selection of salt tolerant cell lines in Solanum tuberosum L.

Biologia Plantarum ◽

10.1007/s10535-007-0149-y ◽

2007 ◽

Vol 51 (4) ◽

pp. 728-734 ◽

Cited By ~ 36

Author(s):

F. Queiros ◽

F. Fidalgo ◽

I. Santos ◽

R. Salema

Keyword(s):

Solanum Tuberosum ◽

Cell Lines ◽

In Vitro Selection ◽

Solanum Tuberosum L ◽

Salt Tolerant ◽

Tolerant Cell ◽

Selection Of

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In vitro characterization of manganese toxicity in relation to phosphorus nutrition in potato (Solanum tuberosum L.)

Plant Science ◽

10.1016/j.plantsci.2004.05.022 ◽

2004 ◽

Vol 167 (5) ◽

pp. 977-986 ◽

Cited By ~ 27

Author(s):

D Sarkar ◽

S.K Pandey ◽

K.C Sud ◽

A Chanemougasoundharam

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L ◽

Manganese Toxicity ◽

Phosphorus Nutrition ◽

In Vitro Characterization

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Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro

Planta ◽

10.1007/s00425-011-1540-7 ◽

2011 ◽

Vol 235 (4) ◽

pp. 761-778 ◽

Cited By ~ 43

Author(s):

María Noelia Muñiz García ◽

Verónica Giammaria ◽

Carolina Grandellis ◽

María Teresa Téllez-Iñón ◽

Rita María Ulloa ◽

...

Keyword(s):

Transcription Factor ◽

Solanum Tuberosum ◽

Solanum Tuberosum L ◽

Bzip Transcription Factor

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Genotypic influence on in vitro induction, dormancy length, advancing age and agronomical performance of potato microtubers (Solanum tuberosum L.)

Annals of Applied Biology ◽

10.1111/j.1744-7348.1994.tb04957.x ◽

1994 ◽

Vol 125 (1) ◽

pp. 161-172 ◽

Cited By ~ 15

Author(s):

P RANALLI ◽

M BIZARRI ◽

L BORGHI ◽

M MARI

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L ◽

In Vitro Induction

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Respon Pemberian Aspirin dan Kinetin Terhadap Pembentukan Umbi Mikro Kentang (Solanum tuberosum L.) Secara In Vitro

10.25047/agropross.2017.15 ◽

2017 ◽

Author(s):

Yunita Prameswari ◽

FNU Djenal ◽

FNU Damanhuri

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L

Kebutuhan kentang yang semakin tinggi menyebabkan permintaan semakin meningkat. Rendahnya produksi kentang mengakibatkan berbagai upaya untuk peningkatan produksi terus dilakukan. Penggunaan metode kultur jaringan yaitu metode untuk mengisolasi bagian tanaman seperti protoplasma, sel, sekelompok sel, jaringan dan organ dalam kondisi aseptik, sehingga dapat diperbanyak dan beregenerasi menjadi tanaman utuh dapat dijadikan alternatif pemenuhan kebutuhan. Penelitian ini bertujuan untuk mengetahui kecepatan pembentukan umbi mikro kentang (Solanum Tuberosum L.) varietas granola kembang secarain vitro dengan menggunakan dua faktor dan 3 kali ulangan. Faktor pertama yaitu aspirin dengan tiga taraf (5,10,15) ppm. Faktor kedua yaitu kinetin dalam tiga taraf (8,10,12) ppm. Penelitian menggunakan seluruh propagul kentang yang berumur 30 hari setelah subkultur dan data yang didapat dianalisis menggunakan ANOVA. Hasil penelitian menunjukan bahwa interaksi aspirin dan kinetin tidak berpengaruh terhadap jumlah akar, kedinian umbi, dan bobot umbi. Interaksi perlakuan terbaik bagi pembentukan tunas yaitu A2K1 aspirin 10 ppm dan kinetin 8 ppm sedangkan Interaksi perlakuan terbaik pada parameter jumlah umbi yaituA3K2 aspirin 15 ppm dan kinetin 10.

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Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

JURNAL BIOS LOGOS ◽

10.35799/jbl.9.2.2019.24416 ◽

2019 ◽

Vol 9 (2) ◽

pp. 91

Author(s):

Ghea Dotulong ◽

Stella Umboh ◽

Johanis Pelealu

Keyword(s):

Solanum Tuberosum ◽

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Leaf Extract ◽

Solanum Tuberosum L ◽

In Vitro Toxicity ◽

Potato Plants ◽

Colony Diameter ◽

In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

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