In vitro selection of salt tolerant cell lines in Solanum tuberosum L.

2007 ◽  
Vol 51 (4) ◽  
pp. 728-734 ◽  
Author(s):  
F. Queiros ◽  
F. Fidalgo ◽  
I. Santos ◽  
R. Salema
Keyword(s):  
Solanum Tuberosum ◽  
Cell Lines ◽  
In Vitro Selection ◽  
Solanum Tuberosum L ◽  
Salt Tolerant ◽  
Tolerant Cell ◽  
Selection Of

In vitro selection of salt-tolerant cell lines in kallar grass [Diplachne fusca (L.) Beauv.]

2003 ◽  
Vol 3 (1) ◽  
pp. 49-52 ◽  
Author(s):  
MALEE NANAKORN ◽  
WALAIKARN JIAMJETJAROON ◽  
SRISOM SUWANAWONG ◽  
CHALERMCHAI WONGWATTANA ◽  
IE SUNG SHIM
Keyword(s):  
Cell Lines ◽  
In Vitro Selection ◽  
Salt Tolerant ◽  
Kallar Grass ◽  
Tolerant Cell ◽  
Selection Of

scholarly journals Rapid in vitro selection of salt-tolerant genotypes of the potentially medicinal plant Centaurium maritimum (L.) fritsch

2009 ◽  
Vol 61 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Danijela Misic ◽  
B. Siler ◽  
Biljana Filipovic ◽  
Zorica Popovic ◽  
Suzana Zivkovic ◽  
...  
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Salinity Tolerance ◽  
Growth Phase ◽  
In Vitro Selection ◽  
Growth Phases ◽  
Salt Tolerant ◽  
Variable Response ◽  
Selection Of

We investigated differences of salinity tolerance between 'salt-tolerant' (ST) and 'salt-sensitive' (SS) genotypes of yellow centaury [Centaurium maritimum (L.) Fritsch] selected during the germination phase. The ability of in vitro cultured C. maritimum to complete the whole ontogenetic cycle in less than 6 months enabled us to deterine salinity tolerance during different growth phases. Based on the physiological attributes measured in this study (growth, morphogenesis, photosynthesis, flowering, seed germination), it can be concluded that C. maritimum genotypes differing in salinity tolerance showed a variable response to elevated salt concentrations during both the vegetative and the generative growth phase.

scholarly journals The In Vitro Screening of Drought Tolerant Potatoes (Solanum tuberosum L) of the Center for Tropical Horticulture Studies - IPB collections

2021 ◽  
Vol 26 (2) ◽  
pp. 235-242
Author(s):  
Jane Kathryne Jolanda Laisina ◽  
Awang Maharijaya ◽  
Sobir Sobir ◽  
Agus Purwito
Keyword(s):  
Solanum Tuberosum ◽  
In Vitro Selection ◽  
Solanum Tuberosum L ◽  
Tolerance Test ◽  
In Vitro Screening ◽  
Critical Limit ◽  
Screening Process ◽  
Drought Tolerant ◽  
Four Levels

In supporting the replacement of potato varieties with the varieties that are adaptive to drought, effective and fast methods are needed. One of which is through in vitro screening of drought-tolerant genotypes. The aims of this research were to determine the appropriate concentration of sorbitol as a critical limit in the in vitro screening process of drought-tolerant genotypes, as well as to determine vegetative characteristics that could be used as indicators of in vitro selection and also to obtain in vitro drought-tolerant genotypes. To achieve these objectives, we used single-nodal explants of seven genotypes cultured for six weeks on MS media added with four levels of sorbitol concentration. Of the four treatments, sorbitol level that could distinguish susceptible and tolerant plants was 0.2 M sorbitol and at this concentration, the characteristics that showed significant interactions were plant height and root length, so that both characteristics could be used as selection indicators for the analysis of tolerance tests for the seven genotypes. The results of tolerance test using the analysis of relative decline and stress-susceptibility index (SSI) showed that PKHT4 and PKHT6 were the genotypes that were tolerant in vitro. Keywords: drought tolerant, in vitro, Solanum tuberosum, sorbitol

scholarly journals In vitro selection of winter triticale for salt resistance

1970 ◽  
pp. 247-251
Author(s):  
S. V. Pykalo ◽  
O. V. Dubrovna ◽  
O. A. Demydov
Keyword(s):  
Salt Stress ◽  
Cell Lines ◽  
In Vitro Selection ◽  
High Survival ◽  
Winter Triticale ◽  
Step Type ◽  
Callus Lines ◽  
Selection Of

Aim. To obtain of cell lines and plant-regenerants of winter triticale resistant for salt stress the in vitro selection was carried out. Methods. In order to select resistant to salt stress forms of triticale the efficiency of using direct and step-type in vitro selection with application of selective system based on sodium chloride has been investigated. Results. The direct and step-type in vitro selection was conducted and the selection of callus lines of triticale being resistant to simu-lated salinity was carried out. As a result, from line 38/1296 and variety Obriy respectively, 5 and 4 resistant callus lines were identified that had a high survival rate on the selective medium with 1.2 % NaCl and maintained morphogenetic potential. From the resistant lines plant regenerants were induced and their rearing, rooting and transfer to in vivo condi-tions were optimized. Conclusions. A step-type in vitro selection was more effective, because resulted from the selec-tion more resistant callus forms were identified. First cell lines of winter triticale with resistance to salt stress were de-rived.Keywords: Triticale, in vitro selection, callus, salt stress, resistance.

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