scholarly journals Isolation, Identification and Antimicrobial profile of Aeromonas spp., Pseudomonas spp. and Vibrio spp. from the Nile Tilapia, Oreochromis niloticus in fish farms

2021 ◽  
Vol 25 (3) ◽  
pp. 171-185
Author(s):  
Hala F. Ayoub et al.
2016 ◽  
Vol 51 (10) ◽  
pp. 1744-1750 ◽  
Author(s):  
Rafael Antunes Baggio ◽  
Raphael Orélis-Ribeiro ◽  
Walter Antonio Boeger

Abstract: The objective of this work was to evaluate a Bayesian model-based clustering method to identify the strains of Nile tilapia (Oreochromis niloticus) individuals from fish farms in Southern Brazil. Assignment methods using nine microsatellite loci were applied to differentiate individuals of five reference strains (GIFT, GST, Nilótica, Chitralada, and Saint Peter) and to identify individuals of unknown strains from fish farms near the Itaipu reservoir and in the Uruguay River basin. The procedure assigned the correct strain in more than 90% of the cases and was also able to detect hybrids between strains. The obtained results showed that several fish farms in Southern Brazil cultivate more than one tilapia strain and even interstrain hybrids. The proposed methodology is a reliable tool for the identification of the strain origin of Nile tilapia individuals.


2019 ◽  
Vol 28 (2) ◽  
pp. 229-237
Author(s):  
Fabrício Sado Rodrigues ◽  
Inácio Mateus Assane ◽  
Gustavo Moraes Ramos Valladão ◽  
Fernanda Gomes de Paula ◽  
Caniggia Lacerda Andrade ◽  
...  

Abstract Massive occurrence of trichodinids is frequently accompanied by serious disease in fish farms. In this study, trichodinid species from the gills and skin of Nile tilapia (Oreochromis niloticus) farmed in the central-western region of Brazil (state of Goiás) were morphologically characterized. Dried slides were prepared from the parasites and were impregnated with silver nitrate (2%). Morphometric characteristics were determined and schematic drawings of the denticles were made using photomicrographs produced from the slides. Seven species of trichodinid ectoparasites (Protozoa: Ciliophora: Trichodinidae) were found parasitizing the gills: four of the genus Trichodina Ehrenberg, 1838; one of Tripartiella Lom, 1959; one of Paratrichodina Lom, 1963; and one of Trichodinella Ṧrámek-Huṧek, 1953. On the body surface, three specimens of the genus Trichodina were identified. This study presents new geographical records of trichodinids in Brazil, thus confirming that Trichodina centrostrigeata, Trichodina compacta, Trichodina heterodentata, Paratrichodina africana and Tripartiella orthodens are widely distributed worldwide. Additionally, the first record of the genus Trichodinella in Brazil is presented.


2012 ◽  
pp. 2812-2819 ◽  
Author(s):  
Wanderson Pantoja MF ◽  
Ligia Neves R ◽  
Márcia Dias RD ◽  
Renata Marinho GB ◽  
Daniel Montagner ◽  
...  

ABSTRACTObjective. This study describes the parasitic fauna and relative condition factor (Kn) in Nile tilapia Oreochromis niloticus L. (Cichlidae) from fish farms in the State of Amapá. Material and methods. 123 fish from four fish farms in the state of Amapá, Brazil were necropsied for parasitological and Kn analysis. Results. 64.2% of the examined fish, had the gills infected with Cichlidogyrus tilapiae Paperna, 1960 (Monogenoidea: Dactylogyridae); Ichthyophthirius multifiliis Fouquet, 1876 (Protozoa: Ciliophora), Trichodina Ehrenberg, 1830 and Paratrichodina africana Kazubski & El-Tantawy, 1986 (Protozoa: Trichodinidae). The highest prevalence found corresponded to Monogenoidea C. tilapiae while the lowest corresponded to Trichodinidae. However, I. multifiliis was the parasite that presented the greatest intensity and abundance. The differences found in the infection rates of the different fish farms due to causes further discussed. The parasitism did not influence the relative condition factor (Kn) of fish. This was the first record of P. africana in Brazil and occurred in the Eastern Amazon. Conclusions. In Brazil, Lamproglena sp. is an emerging parasite in the Southern and Southeastern regions, but this crustacean was not found in the Nile tilapia in the State of Amapá. The parasitic infections in Nile tilapia farmed in Brazil are caused by protozoan, monogenoidea, crustacea and digenea species, and the regional differences on their prevalence and intensity rates are discussed in this study.


Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.


2020 ◽  
Vol 21 (3) ◽  
pp. 61-66
Author(s):  
Ola Hashem ◽  
Viola Zaki ◽  
Rawia Adawy

Objective: To study the incidence and seasonal dynamics of different fungi affected freshwater fishes in Lake Manzala with molecular identification of the isolated fungi. Animals: 300 Nile tilapia (Oreochromis niloticus) and 300 catfish (Clarias gariepinus). Design: Descriptive study. Procedures: Random samples of Oreochromis niloticus (O. niloticus) and Clarias gariepinus (C. gariepinus) were collected from Manzala fish farms. Clinical and postmortem examination of fish was applied. Isolation and identification of different fungi were performed by conventional methods. Furthermore, the molecular characterization of isolated fungi was carried out. Results: C. gariepinus had a higher rate of infection with different fungal species than O. niloticus. Aspergillus spp. (Aspergillus niger and Aspergillus flavus) were the most fungal isolated from the examined fishes, followed by Penicillium spp. and Candida albicans. Aspergillus spp were detected in all seasons with a higher rate in summer and spring. A. flavus, A. niger, Penicillium spp. and C.albicans isolates were amplified from both C. gariepinus and O. niloticus at the specified molecular weight using PCR. Conclusion and clinical relevance: Fungal infection affected the fish showing different external and internal lesions, all species of Aspergillus were found in all seasons with a high rate in, hot seasons, summer and spring. The Prevalence of Penicillium and C. albicans were also reported. All fungal isolates were identified on the phenotypic and molecular bases.


2013 ◽  
Vol 37 (10) ◽  
pp. 1460 ◽  
Author(s):  
Huaping ZHU ◽  
Maixin LU ◽  
Zhanghan HUANG ◽  
Fengying GAO ◽  
Xiaoli KE ◽  
...  

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