scholarly journals IN VITRO PROPAGATION AND SECONDARY METABOLITES PRODUCTION IN THE WILD RARE ASPARAGUS APHYLLUS L. PLANT

2021 ◽  
Vol 71 (2) ◽  
pp. 149-161
Author(s):  
Sabha Salman
2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Asmaa A.A. Abdel- Kareem ◽  
H.A. El- Shamy ◽  
A.K. Dawh ◽  
S.G. Gwiefel

The present work was conducted in order to investigate the effect of auxin type (2,4-D and NAA) and concentration (0.00, 0.25, 0.50, 1.00 and 2.00 mg/l) on Balanites aegyptiaca callus cultures growth and production of secondary metabolites. Obtained results demonstrated that supplementation MS medium with 2,4-D at 2.0 mg/l could enhanced and recorded the ultimate values of callus fresh weight, antioxidant activity (%), total flavonoids, total phenolic compounds and total saponins contents and yields of Balanites aegyptiaca L. callus.


Author(s):  
Sushim Kumar Gupta ◽  
Chao-Lin Kuo ◽  
Hung-Chi Chang ◽  
Hsiao-Sung Chan ◽  
Emily Chin-Fun Chen ◽  
...  

Author(s):  
Shahnawaz ◽  
Devendra Kumar Pandey ◽  
Merinashwari Konjengbam ◽  
Padmanabh Dwivedi ◽  
Prabhjot Kaur ◽  
...  

Author(s):  
Vikas Srivastava ◽  
Aksar Ali Chowdhary ◽  
Skalzang Lhamo ◽  
Sonal Mishra ◽  
Shakti Mehrotra

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 447E-447
Author(s):  
Kimberly A. Pickens ◽  
Jan Wolf ◽  
James M. Affolter ◽  
Hazel Y. Wetzstein

Many bromeliad species indigenous to the rain forests of Central and South America are threatened because of over-collection and habitat destruction. Studies were conducted to develop propagation protocols for Tillandsia eizii, a rare ornamental bromeliad of ceremonial significance to the Highland Maya communities in Chiapas, Mexico. We anticipate using in vitro propagation for the conservation of this species with the potential of utilizing bromeliads as an alternative and sustainable forest resource. Protocols were developed for the sterilization and germination of axenic seed. Seedling growth in vitro was assessed and outplanting studies were conducted. Media were evaluated to promote adventitious bud production in experiments using the plant growth regulators naphthaleneacetic acid and benzylaminopurine. Pulse time and duration, as well as the stage of seed development, had a marked effect on bud production. The effects of various potting media on plant growth and survival were assessed. A pure pine bark medium elicited over 95 percent survival. Plants exhibited a “tank-like” morphology characteristic of plants in the wild.


2014 ◽  
Vol 8 (2) ◽  
pp. 35-43
Author(s):  
Emad H. Jassim ◽  
Sami K. M. Ameen

An experiment was conducted to steady the effect of L-Tryptophan and salicylic acid on callus induced on leaf explants of Catharanthus roseus. Callus induction was achieved by culturing true leaves of the plant on Murashige and Skoog (MS) medium supplemented with 0.5 mg /L 2,4-D and 1mg / L Kin. The best medium to maintain callus was MS medium supplemented with 0.5 mg /L 2,4-D and 1.5 mg / L Kin. Different levels added to MS medium for each L-Tryptophan 0,200,300or400 mg /L and salicylic acid 0, 0.5,1or1.5mg /L. The medium supplemented with 30 g/ L sucrose was used as a control. Results showed the medium supplemented with 200 mgL of L-Tryptophan gave the highest quantity of Vincristine reached 48.66 µg/100 fresh weight of callus. MS medium content at the concentration 1 mg L of salicylic acid gave the highest level of Vinblastine recording 50.98 µg/100 fresh weight of callus. While MS medium supplemented with 0.5 mg /L of salicylic acid gave the highest level of Serpentine 24.76 µg/100 fresh weight of callus. The concentrations of active compound derived from plant leaves were much less than the concentrations produced by the callus grown in vitro. The concentration of Serpentine was 0.059 while Vinblastine was 0.183 and the concentration of Vincristine was 0.064.


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