In vitro propagation and secondary metabolites production in wild germander (Teucrium polium L.)

2011 ◽  
Vol 47 (4) ◽  
pp. 496-505 ◽  
Author(s):  
Tamara S. Al-Qudah ◽  
Rida A. Shibli ◽  
Feras Q. Alali
2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Asmaa A.A. Abdel- Kareem ◽  
H.A. El- Shamy ◽  
A.K. Dawh ◽  
S.G. Gwiefel

The present work was conducted in order to investigate the effect of auxin type (2,4-D and NAA) and concentration (0.00, 0.25, 0.50, 1.00 and 2.00 mg/l) on Balanites aegyptiaca callus cultures growth and production of secondary metabolites. Obtained results demonstrated that supplementation MS medium with 2,4-D at 2.0 mg/l could enhanced and recorded the ultimate values of callus fresh weight, antioxidant activity (%), total flavonoids, total phenolic compounds and total saponins contents and yields of Balanites aegyptiaca L. callus.


Author(s):  
Sushim Kumar Gupta ◽  
Chao-Lin Kuo ◽  
Hung-Chi Chang ◽  
Hsiao-Sung Chan ◽  
Emily Chin-Fun Chen ◽  
...  

Author(s):  
Shahnawaz ◽  
Devendra Kumar Pandey ◽  
Merinashwari Konjengbam ◽  
Padmanabh Dwivedi ◽  
Prabhjot Kaur ◽  
...  

Author(s):  
Vikas Srivastava ◽  
Aksar Ali Chowdhary ◽  
Skalzang Lhamo ◽  
Sonal Mishra ◽  
Shakti Mehrotra

2014 ◽  
Vol 8 (2) ◽  
pp. 35-43
Author(s):  
Emad H. Jassim ◽  
Sami K. M. Ameen

An experiment was conducted to steady the effect of L-Tryptophan and salicylic acid on callus induced on leaf explants of Catharanthus roseus. Callus induction was achieved by culturing true leaves of the plant on Murashige and Skoog (MS) medium supplemented with 0.5 mg /L 2,4-D and 1mg / L Kin. The best medium to maintain callus was MS medium supplemented with 0.5 mg /L 2,4-D and 1.5 mg / L Kin. Different levels added to MS medium for each L-Tryptophan 0,200,300or400 mg /L and salicylic acid 0, 0.5,1or1.5mg /L. The medium supplemented with 30 g/ L sucrose was used as a control. Results showed the medium supplemented with 200 mgL of L-Tryptophan gave the highest quantity of Vincristine reached 48.66 µg/100 fresh weight of callus. MS medium content at the concentration 1 mg L of salicylic acid gave the highest level of Vinblastine recording 50.98 µg/100 fresh weight of callus. While MS medium supplemented with 0.5 mg /L of salicylic acid gave the highest level of Serpentine 24.76 µg/100 fresh weight of callus. The concentrations of active compound derived from plant leaves were much less than the concentrations produced by the callus grown in vitro. The concentration of Serpentine was 0.059 while Vinblastine was 0.183 and the concentration of Vincristine was 0.064.


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