scholarly journals ANTIFUNGAL ACTIVITY OF PLANT EXTRACTS, ESSENTIAL OILS, AND MICROBIAL CULTURE FILTRATES AGAINST Botrytis cinerea In-vitro

2015 ◽  
Vol 6 (9) ◽  
pp. 1297-1311 ◽  
Author(s):  
Y. Shabana ◽  
M. El-Boray ◽  
M. Mustafa ◽  
G. Al-Juboori
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Botrytis Cinerea ◽  
Plant Extracts ◽  
Microbial Culture ◽  
Culture Filtrates

scholarly journals Rapid Evaluation of Plant Extracts and Essential Oils for Antifungal Activity Against Botrytis cinerea

Plant Disease ◽  
1997 ◽  
Vol 81 (2) ◽  
pp. 204-210 ◽  
Author(s):  
C. L. Wilson ◽  
J. M. Solar ◽  
A. El Ghaouth ◽  
M. E. Wisniewski
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Botrytis Cinerea ◽  
Plant Extracts ◽  
Microtiter Plate ◽  
Rapid Evaluation ◽  
Cinnamomum Zeylanicum ◽  
Microtiter Plates ◽  
Plate Reader ◽  
High Antifungal Activity

A rapid assay to determine antifungal activity in plant extracts and essential oils is described. Wells in microtiter plates were loaded with Botrytis cinerea spores and plant extracts or essential oils. Subsequent changes in optical density following spore germination in the wells was measured after 24 h using an automatic microtiter plate reader driven by a software program developed for this purpose. Extracts from 345 plants and 49 essential oils were evaluated for their antifungal activity against B. cinerea. Among 345 plant extracts analyzed, 13 showed high levels of antifungal activity, with species of Allium and Capsicum predominating. Among the 49 essential oils tested, palmarosa (Cymbopogon martini), red thyme (Thymus zygis), cinnamon leaf (Cinnamomum zeylanicum), and clove buds (Eugenia caryophyllata) demonstrated the most antifungal activity against B. cinerea. The most frequently occurring constituents in essential oils showing high antifungal activity were: D-limonene, cineole; β-myrcene; α-pinene, β-pinene; and camphor.

scholarly journals Antifungal activity of three Chilean plant extracts on Botrytis cinerea

2012 ◽  
Vol 90 (2) ◽  
pp. 179 ◽  
Author(s):  
Sofia Vio-Michaelis ◽  
Gastón Apablaza-Hidalgo ◽  
Miguel Gómez ◽  
Raúl Peña-Vera ◽  
Gloria Montenegro
Keyword(s):  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
Plant Extracts

Se estudiaron los posibles efectos antifúngico de los extractos metanólicos crudos y fracciones etanólicas de tres plantas nativas de Chile: Ephedra breana, Fabiana imbricata y Nolana sedifolia sobre el desarrollo micelial in vitro del hongo fitopatógeno Botritis cinerea (moho gris), a una concentración de 250 μg/mL de E. breana y N. sedifolia, y de 400 μg/mL para F. imbricata. Los resultados ponen en evidencia que las fracciones etanólicas de E. breana y N. sedifolia ejercieron un efecto inhibitorio del crecimiento micelial de B. cinerea, en condiciones in vitro. Este efecto demostró ser fungistático y se mantuvo durante los 14 días evaluados. Los extractos completos de ambas especies no mostraron efectos inhibitorios. Los extractos completos y las fracciones etanólicas fueron analizadas por cromatografía líquida de alta eficiencia y entre los compuestos encontrados fueron: ácido clorogénico, ácido cinámico, ácido p-cumárico, ácido ferúlico, vainillina, ácido vainíllico, rutina, ácido caféico, ácido 3,4-hidroxibenzoico (ácido verátrico), ácido caféico dimetil ester (ácido 3, 4 dimetoxicinámico) y ácido protocatéquico. Las fracciones etanólicas obtenidas pueden considerarse como una promisoria alternativa para el control de este fitopatógeno.

Influences of Culture Media, Temperature and Light/Dark Conditions on Growth and Antifungal Activity of Streptomyces spp. Against Botrytis cinerea, in vitro and on Tomato Leaf

2021 ◽  
Author(s):  
Sawai Boukaew ◽  
Siriporn Yossan ◽  
Benjamas Cheirsilp ◽  
Wanida Petlamul ◽  
Poonsuk Prasertsan
Keyword(s):  
Antifungal Activity ◽  
Botrytis Cinerea ◽  
Mycelial Growth ◽  
Culture Media ◽  
Tomato Leaf ◽  
Antifungal Compounds ◽  
Streptomyces Spp ◽  
Culture Filtrates ◽  
Dark Conditions

Abstract The influences of culture media, temperature, and light/dark conditions on growth and antifungal activity of the three strains Streptomyces spp. against Botrytis cinerea was studied. Results of in vitro study indicated that the GYM agar and incubated at 28°C exhibited good mycelial growth and a spore mass production of the three strains of Streptomyces spp. On the other hand, the PDA and incubation at 21°C were suitable for the mycelial growth of B. cinerea . Moreover, light/dark conditions had an effect on the growth of the two strains of S. philanthi . The strains RL-1-178 and RM-1-138 of S. philanthi grown in all media and incubation temperatures tested possessed antifungal activity against B. cinerea (100% inhibition) while S. mycarofaciens showed different results on PDA (83% inhibition) and GYM (88% inhibition) with the optimum incubation temperature at 21°C. Then, the antifungal compounds in culture filtrates produced by the three antagonistic strains against B. cinerea were tested on tomato leaf. They showed a significantly higher inhibitory effect on the symptoms of blight disease on tomato leaf compared with the control. The better protection efficacy against B. cinerea on tomato leaf was observed with the culture filtrates of S. philanthi RM-1-138 (82.89% and 0.33 cm 2 lesion areas symptoms). Moreover, the antifungal compounds in the culture filtrate of S. philanthi RM-1-138, identified by GC-MS, were greatly altered relative to concentration components under different temperatures and light/dark conditions tested. Our results clearly demonstrated that the environmental factors have an influence on antifungal activity of the three strains of Streptomyces spp.

scholarly journals Antifungal Activity and Detoxification of Aflatoxins by Plant Extracts: Potential for Food Applications

2018 ◽  
Vol 10 (1) ◽  
pp. 24-32 ◽  
Author(s):  
Bárbara Ponzilacqua ◽  
Carlos Humberto Corassin ◽  
Carlos Augusto Fernandes Oliveira
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Plant Extracts ◽  
Fungal Growth ◽  
Aflatoxin Contamination ◽  
Attractive Alternative ◽  
Low Concentrations ◽  
Food Applications ◽  
Aflatoxin B

Aflatoxins are secondary metabolites produced by fungi of the genusAspergillus, which occur naturally in cereals like corn, beans and rice. Aflatoxin B1causes an extensive number of toxic effects in animals and humans. This mycotoxin is a stable term and can act in low concentrations due to their higher toxicity. Management to prevent commodities aflatoxin contamination is essential during the production, mainly in pre- and post-harvest steps. A number of essential oils and aqueous plant extracts have been reported to be fungal growth inhibitors and may provide an attractive alternative to prevent aflatoxin contamination in foods. Thus, the aim of this review is to highlight recent data on thein vitroantifungal activity of essential oils and aqueous extracts from plants and discuss the perspectives of their use in food products.

Chemical composition and antifungal activity of essential oils and their combinations against Botrytis cinerea in strawberries

2021 ◽  
Author(s):  
Josemar Gonçalves de Oliveira Filho ◽  
Guilherme da Cruz Silva ◽  
Aline Cristina de Aguiar ◽  
Lavinia Cipriano ◽  
Henriette Monteiro Cordeiro de Azeredo ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Botrytis Cinerea

scholarly journals Chemical constituents, in vitro antibacterial and antifungal activity of Mentha×Piperita L. (peppermint) essential oils

2019 ◽  
Vol 31 (4) ◽  
pp. 528-533 ◽  
Author(s):  
Nagarjuna Reddy Desam ◽  
Abdul Jabbar Al-Rajab ◽  
Mukul Sharma ◽  
Mary Moses Mylabathula ◽  
Ramachandra Reddy Gowkanapalli ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Mentha Piperita ◽  
Antibacterial And Antifungal Activity ◽  
Antibacterial And Antifungal

scholarly journals Trichomes Morphology and Essential Oils Characterization of Field-Growing and In Vitro Propagated Plants of Lavandula pedunculata

2008 ◽  
Vol 14 (S3) ◽  
pp. 148-149 ◽  
Author(s):  
M. Zuzarte ◽  
A.M. Dinis ◽  
C. Cavaleiro ◽  
J. Canhoto ◽  
L. Salgueiro
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Vegetative Propagation ◽  
Leaf Trichomes ◽  
Economic Exploitation ◽  
Selection Of

The selection of native Lavandula species and their economic exploitation have increased in the last few years. Micropropagation techniques have been used as an alternative for vegetative propagation allowing the multiplication of selected genotypes and chemotypes. Our previous studies showed that the essential oils of Lavandula pedunculata have an important antifungal activity against dermatophyte strains. Therefore, a new line of investigation concerning the in vitro culture of this species is justified. In the present study we compare the morphology of the leaf trichomes and the chemical composition of their essential oils in both field-growing and in vitro propagated plants.

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