Influence of electron donor and toxic materials on the activity of sulfate reducing bacteria for the treatment of electroplating wastewater

1998 ◽  
Vol 38 (4-5) ◽  
pp. 187-194 ◽  
Author(s):  
Young-Chae Song ◽  
Byeong-Cheon Piak ◽  
Hang-Sik Shin ◽  
Sung-Jin La
Keyword(s):  
Carbon Source ◽  
Electron Donor ◽  
Sulfate Reducing Bacteria ◽  
Efficient Treatment ◽  
Electroplating Wastewater ◽  
Inhibition Model ◽  
Sulfate Reducing ◽  
Chromium Vi ◽  
Toxic Materials ◽  
Reducing Bacteria

The effects of electron donor and toxic materials on the activity of SRB (sulfate reducing bacteria) were evaluated to ensure the efficient treatment of electroplating wastewater using an anaerobic batch test. The maximum utilization rate of glucose by SRB was slightly slower than that of lactate and acetate, but the affinity of glucose on the SRB was similar to that of the acetate and the lactate. The concentration of carbon source required to reduce 2,000mg/L of sulfate, that is the common concentration of sulfate in the raw electroplating wastewater, was estimated as about 2,200mg COD/L. The efficiency of carbon source for the reduction of sulfate was the highest at around 0.33 of the COD/SO4−2 ratio, but the rate of sulfate reduction was increased according to the increase of COD/SO4−2 ratio ranged from 0.18 to 1.21. Inhibition of copper on the activity of SRB was able to explain using non-competitive inhibition model, and IC50 for copper, that is the concentration causing 50% inhibition of SRB activity, was about 100mg/L of copper. The inhibition of chromium(VI) on the activity of SRB was observed as a mixed inhibition, but the degree of inhibition on the activity of SRB was only about 15% at 130mg/L of the chromium(VI). The inhibitory effect on the SRB caused by 10mg/L of cyanide compound was over 30% of the control, and it was rapidly increased with the cyanide concentration. That could be explained using uncompetitive inhibition model, and IC50 was estimated as about 20mg/L.

Arsenic removal and activity of a sulfate reducing bacteria-enriched anaerobic sludge using zero valent iron as electron donor

2020 ◽  
Vol 384 ◽  
pp. 121392 ◽  
Author(s):  
Olga Lidia Zacarías-Estrada ◽  
Lourdes Ballinas-Casarrubias ◽  
María Elena Montero-Cabrera ◽  
Rene Loredo-Portales ◽  
Erasmo Orrantia-Borunda ◽  
...  
Keyword(s):  
Electron Donor ◽  
Arsenic Removal ◽  
Sulfate Reducing Bacteria ◽  
Zero Valent Iron ◽  
Anaerobic Sludge ◽  
Sulfate Reducing ◽  
Reducing Bacteria

scholarly journals Activity and Diversity of Sulfate-Reducing Bacteria in a Petroleum Hydrocarbon-Contaminated Aquifer

2002 ◽  
Vol 68 (4) ◽  
pp. 1516-1523 ◽  
Author(s):  
Jutta Kleikemper ◽  
Martin H. Schroth ◽  
William V. Sigler ◽  
Martina Schmucki ◽  
Stefano M. Bernasconi ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sulfate Reduction ◽  
Petroleum Hydrocarbon ◽  
Carbon Sources ◽  
Sulfate Reducing Bacteria ◽  
Rate Coefficients ◽  
Contaminated Aquifer ◽  
Sulfate Reducing ◽  
Pull Tests ◽  
Reducing Bacteria

ABSTRACT Microbial sulfate reduction is an important metabolic activity in petroleum hydrocarbon (PHC)-contaminated aquifers. We quantified carbon source-enhanced microbial SO4 2− reduction in a PHC-contaminated aquifer by using single-well push-pull tests and related the consumption of sulfate and added carbon sources to the presence of certain genera of sulfate-reducing bacteria (SRB). We also used molecular methods to assess suspended SRB diversity. In four consecutive tests, we injected anoxic test solutions (1,000 liters) containing bromide as a conservative tracer, sulfate, and either propionate, butyrate, lactate, or acetate as reactants into an existing monitoring well. After an initial incubation period, 1,000 liters of test solution-groundwater mixture was extracted from the same well. Average total test duration was 71 h. We measured concentrations of bromide, sulfate, and carbon sources in native groundwater as well as in injection and extraction phase samples and characterized the SRB population by using fluorescence in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE). Enhanced sulfate reduction concomitant with carbon source degradation was observed in all tests. Computed first-order rate coefficients ranged from 0.19 to 0.32 day−1 for sulfate reduction and from 0.13 to 0.60 day−1 for carbon source degradation. Sulfur isotope fractionation in unconsumed sulfate indicated that sulfate reduction was microbially mediated. Enhancement of sulfate reduction due to carbon source additions in all tests and variability of rate coefficients suggested the presence of specific SRB genera and a high diversity of SRB. We confirmed this by using FISH and DGGE. A large fraction of suspended bacteria hybridized with SRB-targeting probes SRB385 plus SRB385-Db (11 to 24% of total cells). FISH results showed that the activity of these bacteria was enhanced by addition of sulfate and carbon sources during push-pull tests. However, DGGE profiles indicated that the bacterial community structure of the dominant species did not change during the tests. Thus, the combination of push-pull tests with molecular methods provided valuable insights into microbial processes, activities, and diversity in the sulfate-reducing zone of a PHC-contaminated aquifer.

scholarly journals Reduction of chromium (VI) compounds by sulfate-reducing bacteria

2010 ◽  
Vol 4 (2) ◽  
pp. 39-48
Author(s):  
T. B. Peretyatko ◽  
◽  
S. P. Gudz ◽  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Chromium Vi ◽  
Reducing Bacteria

scholarly journals Simulation Study on Reservoir Souring Induced by Injection of Reservoir Brine Containing Sulfate-Reducing Bacteria

2020 ◽  
Vol 12 (11) ◽  
pp. 4603
Author(s):  
Yuichi Sugai ◽  
Yukihiro Owaki ◽  
Kyuro Sasaki
Keyword(s):  
Electron Donor ◽  
Sulfate Reducing Bacteria ◽  
Mixing Zone ◽  
Formation Water ◽  
Sulfate Reducing ◽  
Injection Water ◽  
High Level ◽  
Heating Up ◽  
Reducing Bacteria ◽  
Brine Injection

This paper examined the reservoir souring induced by the sulfate-reducing bacteria (SRB) inhabiting the reservoir brine of an oilfield in Japan. Although the concentration of sulfate of the reservoir brine was lower than that of seawater, which often was injected into oil reservoir and induced the reservoir souring, the SRB inhabiting the reservoir brine generated hydrogen sulfide (H2S) by using sulfate and an electron donor in the reservoir brine. This paper therefore developed a numerical simulator predicting the reservoir souring in the reservoir into which the reservoir brine was injected. The results of the simulation suggested that severe reservoir souring was not induced by the brine injection; however, the SRB grew and generated H2S around the injection well where temperature was decreased by injected brine whose temperature was lower than that of formation water. In particular, H2S was actively generated in the mixing zone between the injection water and formation water, which contained a high level of the electron donor. Furthermore, the results of numerical simulation suggested that the reservoir souring could be prevented more surely by sterilizing the SRB in the injection brine, heating up the injection brine to 50 °C, or reducing sulfate in the injection brine.

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